FlyBase Gene Report: Dmel\bun

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Gene: Dmel\bun

General Information

Symbol

Dmel\bun

Species

D. melanogaster

Name

bunched

Annotation Symbol

CG42281

Feature Type

protein_coding_gene

FlyBase ID

FBgn0259176

Gene Model Status

Stock Availability

148 publicly available

Gene Summary

Probable transcription factor required for peripheral nervous system morphogenesis, eye development and oogenesis. May be required for the transmission of the dpp signal and for a morphogenetic movement of the medulla in the brain that reorients the second optic lobe relative to the first. Plays a role in determining proper dorsal cell fates leading to the formation of the dorsal appendages. (UniProt, Q24522 Q24523)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

shs, l(2)00255, TSC-22, Dm_2L:60722

Key Links

Genomic Location

Cytogenetic map

Sequence location

2L:12,455,540..12,546,611 [-]

Recombination map

2-46

RefSeq locus

NT_033779 REGION:12455540..12546611

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables protein homodimerization activity

inferred from sequence or structural similarity

(Dobens et al., 1997)

Biological Process (14 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

involved_in chorion-containing eggshell formation

inferred from mutant phenotype

(Dobens et al., 2000)

involved_in compound eye photoreceptor cell differentiation

inferred from mutant phenotype

(Treisman et al., 1995)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Kim et al., 2009)

involved_in dorsal appendage formation

inferred from mutant phenotype

(Dobens et al., 1997)

involved_in follicle cell of egg chamber migration

inferred from mutant phenotype

(Dobens et al., 2005)

involved_in intestinal stem cell homeostasis

inferred from mutant phenotype

(Nie et al., 2015)

involved_in mushroom body development

inferred from mutant phenotype

(Kim et al., 2009)

involved_in negative regulation of apoptotic process

inferred from mutant phenotype

(Wu et al., 2008)

involved_in negative regulation of cell fate specification

inferred from mutant phenotype

(Dobens et al., 2000)

involved_in positive regulation of cell growth

inferred from mutant phenotype

(Gluderer et al., 2008, Wu et al., 2008)

involved_in positive regulation of cell population proliferation

inferred from mutant phenotype

(Wu et al., 2008)

involved_in positive regulation of neuroblast proliferation

inferred from mutant phenotype

(Kim et al., 2009)

involved_in tissue regeneration

inferred from mutant phenotype

(Gerhold et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in negative regulation of apoptotic process

inferred from biological aspect of ancestor with PANTHER:PTN002928800

(GO Reference Genome Project, 2011-)

involved_in positive regulation of cell population proliferation

inferred from biological aspect of ancestor with PANTHER:PTN002928800

(GO Reference Genome Project, 2011-)

involved_in regulation of transcription by RNA polymerase II

inferred from electronic annotation with InterPro:IPR000580

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Treisman et al., 1995)

inferred from high throughput direct assay

(Lye et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN002928800

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002928800

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED DNA BINDING DOMAIN TRANSCRIPTION FACTORS

Protein Family (UniProt)

Belongs to the TSC-22/Dip/Bun family. (Q24522 Q24523)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

UNCLASSIFIED DNA BINDING DOMAIN TRANSCRIPTION FACTORS -: This group comprises DNA-binding transcription factors that do not classify under other domain-based transcription factor groups in FlyBase.

Protein Function (UniProtKB)

Probable transcription factor required for peripheral nervous system morphogenesis, eye development and oogenesis. May be required for the transmission of the dpp signal and for a morphogenetic movement of the medulla in the brain that reorients the second optic lobe relative to the first. Plays a role in determining proper dorsal cell fates leading to the formation of the dorsal appendages.

(UniProt, Q24522 Q24523)

Summary (Interactive Fly)

transcription factor - leucine zipper - Dpp pathway - mutation leads to a delay in differentiation and to a loss of photoreceptors - required for a morphogenetic movement in the brain - functions in wing margin development - required for both male and female fertility

Interactive Fly

Gene Model and Products

Number of Transcripts

10

Number of Unique Polypeptides

9

Please see the JBrowse view of Dmel\bun for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q24523)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.49

Gene model reviewed during 5.43

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.47

Gene model reviewed during 5.57

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0300521

5938

1206

FBtr0299656

2728

219

FBtr0299657

1754

189

FBtr0299652

3668

226

FBtr0300522

2832

254

FBtr0300523

7023

1097

FBtr0299658

5921

1331

FBtr0300524

2106

226

FBtr0339528

3490

192

FBtr0346622

5139

1137

Additional Transcript Data and Comments

Reported size (kB)

5.8, 2.8 (longest cDNA)

(Treisman et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0289748

124.8

1206

6.27

FBpp0288931

22.7

219

4.21

FBpp0288932

19.7

189

4.42

FBpp0288927

23.5

226

4.35

FBpp0289749

26.4

254

4.63

FBpp0289750

113.4

1097

6.09

FBpp0288933

138.1

1331

6.24

FBpp0289751

23.5

226

4.35

FBpp0308611

19.6

192

4.35

FBpp0312202

118.5

1137

6.51

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

226 aa isoforms: bun-PD, bun-PH

Additional Polypeptide Data and Comments

Reported size (kDa)

1212, 224 (aa)

(Treisman et al., 1995)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bun using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.49

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

amnioserosa anlage

(Fisher et al., 2012)

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

(Fisher et al., 2012)

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

(Fisher et al., 2012)

amnioserosa primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

proventriculus primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

(Lee et al., 2003)

Additional Descriptive Data

The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue.

(Lee et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

eye-antennal disc

(Treisman et al., 1995)

alpha/beta Kenyon cell

(Kim et al., 2009)

(Kim et al., 2009)

alpha/beta Kenyon cell

(Kim et al., 2009)

(Kim et al., 2009)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

bun is expressed in mushroom body neurons during metamorphosis and is greatly reduced in adults. They are expressed in the alpha/beta neurons but not in alpha neurons or alpha'/beta' neurons.

(Kim et al., 2009)

bun protein is expressed in a stripe just anterior to the morphogenetic furrow.

(Treisman et al., 1995)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Treisman et al., 1995)

inferred from high throughput direct assay

(Lye et al., 2014)

Expression Deduced from Reporters

Reporter: P{PZ}bun⁰⁰²⁵⁵

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

(Meister and Braun, 1995.10)

(Meister and Braun, 1995.10)

embryonic/larval salivary gland

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

adult Malpighian tubule principal cell

(Wang et al., 2004)

Reporter: P{Switch2}bun^GSG5961

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- third instar larval stage

midgut imaginal island

(Mathur et al., 2010)

intestinal stem cell

(Resnik-Docampo et al., 2021, Filer et al., 2017, Foronda et al., 2014)

(Resnik-Docampo et al., 2021)

Reporter: PBac{806.LOX-SVS-2}bun^CPTI004088

Stage

Tissue/Position (including subcellular localization)

Reference

ellipsoid body | faint | restricted

Comment: inner part of ellipsoid body

(Knowles-Barley, 2011.8.24)

adult antennal lobe | restricted

(Knowles-Barley, 2011.8.24)

adult mushroom body | restricted

(Knowles-Barley, 2011.8.24)

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\bun in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\bun

View exonic expression by tissue for Dmel\bun

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259176

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34665/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259176

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259176

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259176

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5461

Flygut - An atlas of the Drosophila adult midgut

FBgn0259176

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 164 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 22 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of bun

Transgenic constructs containing regulatory region of bun

Aberrations (Deficiencies and Duplications) ( 12 )

Inferred from experimentation ( 12 )

Gene disrupted in

(Christensen et al., 2008.4.15)

(Wu et al., 2008)

(Ryder, 2004.4.26)

(Gluderer et al., 2008, BDGP Project Members, 1994-1999)

(Gluderer et al., 2008, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Gene not disrupted in

(Kania et al., 1995, BDGP Project Members, 1994-1999)

(BDGP Project Members, 1994-1999)

(Butler, 2001)

(Butler, 2001)

(Kania et al., 1995)

(BDGP Project Members, 1994-1999)

(Butler, 2001)

In(2LR)TE35B-4^LTE35B-14^R

(BDGP Project Members, 1994-1999)

Inferred from location ( 11 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

bun^MI02911

lethal, with Scer\GAL4^Act5C.PU

lethal (with bun⁰⁴²³⁰)

lethal (with bun⁰⁶⁹⁰³)

lethal (with bun^k02903)

lethal (with bun^out4)

lethal (with bun^out12)

lethal (with bun^out14)

lethal (with bun^rI043)

lethal (with Df(2L)prd1.7)

lethal | larval stage | recessive

bun⁰⁰²⁵⁵

lethal | maternal effect | recessive

bun^rI043

lethal | second instar larval stage (with Df(2L)Exel6033)

lethal | third instar larval stage (with Df(2L)Exel6033)

lethal - all die before end of larval stage | recessive

lethal - all die before end of pupal stage | recessive

bun^k09036

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

bun^GD1392

partially lethal - majority die, with Scer\GAL4^pnr-MD237

bun^GD1392

partially lethal - majority live (with bun^out4)

bun^rI043

partially lethal - majority live (with bun^out12)

bun^rI043

partially lethal - majority live (with bun^out14)

bun^rI043

partially lethal - majority live (with bun^rI043)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

bun^GD10387

viable, with Scer\GAL4^Mef2.PR

bun^GD10387

viable, with Scer\GAL4^pnr-MD237

bun^GD10387

viable, with Scer\GAL4^tin.CΔ4

bun^GD1392

Sterility

Allele

female sterile | recessive

bun^rI043

Other Phenotypes

Allele

abnormal cell shape | adult stage, with Scer\GAL4^NP5130

bun^GD1392

abnormal cell shape | somatic clone

bun^unspecified

abnormal developmental rate

bun^GE12327

abnormal developmental rate | larval stage (with Df(2L)Exel6033)

abnormal locomotor behavior | progressive

bun^M15

abnormal size | adult stage, with Scer\GAL4^nub.PU

bun^JF02954

decreased body size (with bun^out4)

bun^rI043

decreased body size (with bun^out12)

bun^rI043

decreased body size (with bun^out14)

bun^rI043

decreased body size (with bun^rI043)

decreased body size | adult stage | dominant | female

bun^A-149B

decreased body size | adult stage | dominant | male

bun^A-149B

decreased body size | adult stage | female | recessive

bun^GE12327

decreased body size | female (with bun^A-149B)

bun^GE12327

decreased body size | female (with bun^GE12327)

bun^A-149B

decreased body size | larval stage (with Df(2L)Exel6033)

bun^200B

decreased body size | male (with bun^A-149B)

bun^GE12327

decreased body size | male (with bun^GE12327)

bun^A-149B

decreased cell number

decreased cell number (with bun^A-149B)

bun^GE12327

decreased cell number (with bun^GE12327)

bun^A-149B

decreased cell number | adult stage, with Scer\GAL4^NP5130

bun^GD1392

decreased cell number | adult stage | somatic clone

bun^200B

decreased cell number | dominant

bun^A-149B

decreased cell number | larval stage

bun^A5G3

decreased cell number | recessive | somatic clone

bun^A-Q578X

decreased cell number | somatic clone

decreased cell size

bun^GE12327

decreased cell size (with bun^A-149B)

bun^GE12327

decreased cell size (with bun^GE12327)

bun^A-149B

decreased cell size | dominant

bun^A-149B

decreased cell size | recessive | somatic clone

bun^A-Q578X

decreased cell size | somatic clone

bun^A-Q922X

decreased occurrence of cell division | adult stage, with Scer\GAL4^Delta.PU

bun^GD1392

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

bun^GD1392

decreased occurrence of cell division | P-stage

bun^M15

bun^M15

some die during embryonic stage | rescuable maternal effect

bun⁰⁰²⁵⁵

some die during larval stage | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

bun^GD1392

some die during pupal stage | recessive

bun^k09036

some die during third instar larval stage | recessive

bun^200B

bun^rI043

visible, with Scer\GAL4^Bx-MS1096

bun^{UAS.ORF.GW.Tag:HA}

visible, with Scer\GAL4^pnr-MD237

bun^GD1392

visible | conditional

bun^A5G3

visible | recessive | somatic clone

bun^cDNA2.GMR

Phenotype manifest in

Allele

adult head | somatic clone

adult midgut, with Scer\GAL4^Delta.PU

bun^GD1392

adult midgut, with Scer\GAL4^NP5130

bun^GD1392

adult mushroom body alpha-lobe

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

adult mushroom body alpha-lobe (with bun^G12085)

adult mushroom body alpha-lobe (with bun^G12937)

adult mushroom body alpha-lobe (with bun^M15)

adult mushroom body beta-lobe

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

adult mushroom body beta-lobe (with bun^G12085)

adult mushroom body beta-lobe (with bun^G12937)

adult mushroom body beta-lobe (with bun^M15)

centripetal follicle cell, with Scer\GAL4^Act5C.PP

bun^B.UAS

centripetal follicle cell | somatic clone

bun^unspecified

chordotonal organ | lateral

bun^k09036

dorsal appendage

bun^rI043

dorsal appendage | maternal effect | somatic clone

egg | maternal effect | somatic clone

egg chamber | somatic clone

bun^unspecified

bun^rI043

embryonic/first instar larval cuticle

bun⁰⁰²⁵⁵

embryonic segment

bun⁰⁰²⁵⁵

enteroblast | adult stage, with Scer\GAL4^NP5130

bun^GD1392

enteroendocrine cell | adult stage, with Scer\GAL4^NP5130

bun^GD1392

eye | conditional

bun^A5G3

eye disc | conditional

bun^A5G3

follicle cell, with Scer\GAL4^Act5C.PP

bun^X.UAS

germline cell | male, with Scer\GAL4^ptc-559.1

bun^EP2622

intestinal stem cell | adult stage, with Scer\GAL4^NP5130

bun^GD1392

intestinal stem cell | adult stage | somatic clone

bun^200B

bun^M15

leg (with bun^out4)

bun^rI043

leg (with bun^out12)

bun^rI043

leg (with bun^out14)

bun^rI043

leg (with bun^rI043)

main body follicle cell | somatic clone

bun^unspecified

mesonotum, with Scer\GAL4^pnr-MD237

bun^GD1392

bun^M15

ommatidium (with bun^A-149B)

bun^GE12327

ommatidium (with bun^A-Q578X)

bun^GE12327

ommatidium (with bun^GE12327)

ommatidium | somatic clone

photoreceptor cell R3 (with bun^A-Q578X)

bun^GE12327

photoreceptor cell R3 (with bun^GE12327)

bun^A-Q578X

photoreceptor cell R4 (with bun^A-Q578X)

bun^GE12327

photoreceptor cell R4 (with bun^GE12327)

bun^A-Q578X

photoreceptor cell R7

bun^rI043

photoreceptor cell R8

bun^rI043

rhabdomere | somatic clone

bun⁰⁰²⁵⁵

trichogen cell, with Scer\GAL4^pnr-MD237

bun^GD1392

bun^rI043

wing, with Scer\GAL4^Bx-MS1096

bun^{UAS.ORF.GW.Tag:HA}

wing, with Scer\GAL4^nub.PU

bun^JF02954

wing disc | somatic clone

wing vein (with bun^out4)

bun^rI043

wing vein (with bun^out12)

bun^rI043

wing vein (with bun^out14)

bun^rI043

wing vein (with bun^rI043)

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (4)

9 of 14

Yes

Yes

2

6 of 14

No

Yes

1

6 of 14

No

Yes

1

6 of 14

No

Yes

1

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

6 of 14

Yes

Yes

6 of 14

Yes

Yes

5 of 14

No

Yes

5 of 14

No

Yes

Rnor\C12H7orf61

1 of 14

No

Yes

Mus musculus (laboratory mouse) (4)

7 of 14

Yes

Yes

6 of 14

No

Yes

6 of 14

No

Yes

6 of 14

No

Yes

Xenopus tropicalis (Western clawed frog) (5)

6 of 13

Yes

Yes

4 of 13

No

Yes

3 of 13

No

Yes

2 of 13

No

Yes

1 of 13

No

No

Danio rerio (Zebrafish) (4)

6 of 14

Yes

Yes

6 of 14

Yes

Yes

6 of 14

Yes

Yes

6 of 14

Yes

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

3 of 14

Yes

Yes

3 of 14

Yes

Yes

2 of 14

No

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP010044

8 of 12

Yes

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:bun. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TSC22D1; TSC22 domain family member 1

9 of 14

TSC22D2; TSC22 domain family member 2

6 of 14

TSC22D3; TSC22 domain family member 3

6 of 14

TSC22D4; TSC22 domain family member 4

6 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

(Gluderer et al., 2010)

(Gluderer et al., 2010)

(Gluderer et al., 2010)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

two hybrid array

(Shokri et al., 2019)

anti tag coimmunoprecipitation, western blot, affinity technology, Identification by mass spectrometry, two hybrid

(Gluderer et al., 2010)

two hybrid array

(Shokri et al., 2019)

two hybrid array

(Shokri et al., 2019)

two hybrid array

(Shokri et al., 2019)

two hybrid array

(Shokri et al., 2019)

two hybrid array

(Shokri et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Treisman et al., 1995)

enhanceable

(Treisman et al., 1995)

enhanceable

(Treisman et al., 1995)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Treisman et al., 1995)

suppressible

(Nie et al., 2015)

suppressible

(Nie et al., 2015)

suppressible

(Abrell et al., 2000)

suppressible

(Nie et al., 2015)

suppressible

(Pena-Rangel et al., 2002)

suppressible

(Nie et al., 2015)

enhanceable

(Gluderer et al., 2008)

suppressible

(Gluderer et al., 2008)

suppressible

(Hevia et al., 2017)

suppressible

(Mukherjee et al., 2006)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60711

DroID - A comprehensive database of gene and protein interactions.

FBgn0259176

MIST (genetic) - An integrated Molecular Interaction Database

34665

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2L

Recombination map

2-46

Cytogenetic map

Sequence location

2L:12,455,540..12,546,611 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

33E5-33E9

Limits computationally determined from genome sequence between P{EP}Elf^EP476&P{EP}bun^EP488 and P{lacW}bun^k00612&P{EP}EP682^EP682

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

33E7-33E8

(Treisman, 1995.9.29)

33E7-33E8

(determined by in situ hybridisation)

(Spradling et al., 1999, Perrimon et al., 1996)

33E-33E

(determined by in situ hybridisation)

(Dobens et al., 1997)

33F-33F

(determined by in situ hybridisation)

(Kania et al., 1995)

33E9-33E10

33F1--2 38B4--5

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-46

(FlyBase, 2016)

2-41.1

(Comeron et al., 2012)

2-

(Treisman, 1995.9.29)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (148)

Aberrations including deletions of this gene Aberrations including duplications of this gene

11910

P{VT006394-GAL4}attP2

11911

P{VT006395-GAL4}attP2

11912

P{VT006415-GAL4}attP2

12044

P{VT006391-GAL4}attP2

12045

P{VT006410-GAL4}attP2

12046

P{VT006414-GAL4}attP2

12316

P{VT006398-GAL4}attP2

12317

P{VT006412-GAL4}attP2

12318

P{VT006418-GAL4}attP2

12578

P{VT006406-GAL4}attP2

12844

P{VT006393-GAL4}attP2

13366

P{VT006404-GAL4}attP2

More stocks available...

Genomic Clones (55)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (213)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_GH13775

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0259176

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0259176

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kim et al., 2009)

monoclonal

(Treisman et al., 1995)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

bun regulates the level of N stimulation to position the boundary between centripetally migrating and stationary columnar cells.

(Dobens et al., 2005)

New annotation (CG31857) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

bun integrates opposing dpp and EGF signals to set the operculum boundary in the developing egg.

(Dobens et al., 2000)

Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

(Harvie et al., 1998)

bun has been cloned and sequenced.

(Dobens et al., 1997)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.

(Perrimon et al., 1996)

Mutations in bun cause a delay in photoreceptor neural differentiation relative to dpp expression suggesting that bun may be required for the transmission of the dpp signal. bun has a role in inducing rotation of the second optic lobe. bun encodes a cytoplasmic protein expressed anterior to the furrow in a hh-dependent manner.

(Treisman et al., 1995)

Relationship to Other Genes

Source for database merge of

Source for merge of: bun CG31857

(FlyBase Genome Annotators, 2008)

Additional comments

Annotations CG5461 and CG31857 merged as CG42281 in release 5.8 of the genome annotation.

(FlyBase Genome Annotators, 2008)

Nomenclature History

Source for database identify of

Source for identity of: bun CG5461

(Zhong, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

1550

(Harvie et al., 1998)

Bun

(Lepesant et al., 2020, Xu et al., 2016, Tokusumi et al., 2012, Xu and Gridley, 2012, Pastor-Pareja and Xu, 2011, Martin et al., 2007)

BunA

(Gluderer et al., 2010)

CG31857

CG42281

(National Institute of Genetics Fly Stocks, 2022-, Hevia et al., 2017, Hosono et al., 2015, Fukuoh et al., 2014, Hadar et al., 2012, FlyBase Genome Annotators, 2008)

CG5461

(Christis and Munro, 2012, Perkins et al., 2009, Quinones-Coello, 2007, Lee et al., 2003, Whitfield, 2001.1.5)

Dm_2L:60722

(FlyBase Genome Annotators, 2014, Ladoukakis et al., 2011)

EP(2)0488

(Pena-Rangel et al., 2002)

TSC-22

(Gluderer et al., 2010, Dobens et al., 1995)

Tsc22

bnc

(Wang et al., 2004)

bun

(Sood et al., 2024, Praschberger et al., 2023, Titlow et al., 2023, Ding et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Fan et al., 2021, Slaidina et al., 2021, Hung et al., 2020, Salazar et al., 2020, Kockel et al., 2019, Shokri et al., 2019, Bischof et al., 2018, Gene Disruption Project members, 2018-, Corrales et al., 2017, Fochler et al., 2017, Karaiskos et al., 2017, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Corty et al., 2016, Gene Disruption Project members, 2016-, Lycette et al., 2016, Duff et al., 2015, Gene Disruption Project members, 2015-, Morozova et al., 2015, Nie et al., 2015, Schertel et al., 2015, Van Bortle et al., 2015, Wang et al., 2015, Zwarts et al., 2015, Alic et al., 2014, Ashwal-Fluss et al., 2014, Chen et al., 2014, Saunders et al., 2013, Zimmerman et al., 2013, Hadar et al., 2012, Xu and Gridley, 2012, Abruzzi et al., 2011, Carreira et al., 2011, Gerhold et al., 2011, Luo et al., 2011, Michaut et al., 2011, Pastor-Pareja and Xu, 2011, Seugnet et al., 2011, Spradling et al., 2011, Blanco et al., 2010, Gan et al., 2010, Silicheva et al., 2010, Gluderer et al., 2009.2.9, Yamamoto et al., 2009.2.25, Christensen et al., 2008.4.15, FlyBase Curators, 2008-, Gluderer et al., 2008, Liu and Lehmann, 2008, Wu et al., 2008, Aerts et al., 2007, Ash et al., 2007, Buszczak et al., 2007, Gluderer et al., 2007, Levine et al., 2007, Maines et al., 2007, Neuman-Silberberg, 2007, Quinones-Coello, 2007, Wu et al., 2007, Biemar et al., 2006, Mukherjee et al., 2006)

l(2)00255

(Russell et al., 1998, Perrimon et al., 1996)

l(2)02687

(Abrell et al., 2000)

l(2)05479

(Abrell et al., 2000)

l(2)06475

(Abrell et al., 2000)

l(2)k02903

(Butler, 2001)

l(2)rI043

shs

(Dobens and Raftery, 1997, Dobens and Raftery, 1996, Huang and Kunes, 1996, Moses and Serra, 1996, Treisman, 1995.9.29, Treisman, 1995.9.29, Treisman et al., 1995)

Name Synonyms

Bunched

(Xu et al., 2016, Jones and Srivastava, 2014, Pastor-Pareja and Xu, 2011, Gluderer et al., 2010, Martin et al., 2007)

CG31857

CG42281

(FlyBase Genome Annotators, 2008)

short sighted

short-sighted

(Thummel and Chory, 2002)

shortsighted

(White et al., 1999)

Secondary FlyBase IDs

FBgn0010460
FBgn0010670
FBgn0011724
FBgn0014385
FBgn0051857

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 112 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34665

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q24523

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q24523

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259176

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34665/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259176

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259176

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42281

MARRVEL_MODEL - MARRVEL (model organism gene)

34665

Linkouts

BioGRID - A database of protein and genetic interactions.

60711

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0259176

DroID - A comprehensive database of gene and protein interactions.

FBgn0259176

DRSC - Results frm RNAi screens

FBgn0259176

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259176

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0259176

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5461

Flygut - An atlas of the Drosophila adult midgut

FBgn0259176

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC001602

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

34665

References (215)