FlyBase Insertion Report: Dmel\P{Switch2}bun[GSG5961]

General Information

Symbol

Dmel\P{Switch2}bun^GSG5961

Species

D. melanogaster

Name

FlyBase ID

FBti0150383

Feature type

transposable_element_insertion_site

Also Known As

GS5961, 5961GS, 5961-GS, pSwitch^AMP, 5961-GAL4^GS

Description

Inserted Element

P{Switch2}

LINE ID

Affected gene(s)

bun, Scer\GAL4

Viability / fertility

Associated allele(s)

bun^GSG5961, Scer\GAL4^bun-GSG5961

Stock availability

None publicly available

Genomic Location

Chromosomal Location

2L ( 33E7 )

Sequence Location

2L:12,507,796..12,508,010

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:12,507,796..12,508,010

(Cole et al., 2012.7.6)

Orientation

Cytological location (computed by FlyBase)

33E7 (inferred by FlyBase from sequence location)

Cytological location (reported)

33E7_r4 (reported as inferred from sequence location)

(Cole et al., 2012.7.6)

33E7 (reported as inferred from sequence location)

(Nicholson et al., 2008)

Insertion into Natural transposon

Comments concerning location

Alternative genomic location: 2L:12507776..12508023 (Genome release number: 4)

(Cole et al., 2012.7.6)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{Switch2}

(Nicholson et al., 2008)

Size (Kb)

7.931

(Roman, 2002.6.25)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

bun

(Cole et al., 2012.7.6, Nicholson et al., 2008)

Scer\GAL4

(Cole et al., 2012.7.6, Rera et al., 2011, Biteau et al., 2010, Mathur et al., 2010, Mathur et al., 2010, Nicholson et al., 2008, Nicholson et al., 2008)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

bun^GSG5961

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^bun-GSG5961

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- third instar larval stage

midgut imaginal island

(Mathur et al., 2010)

adult stage

intestinal stem cell

(Resnik-Docampo et al., 2021, Filer et al., 2017, Foronda et al., 2014)

enteroblast

(Resnik-Docampo et al., 2021)

Additional Information

Statement

Reference

Drives mifepristone-inducible expression in intestinal stem cells and enteroblasts in adults, and midgut imaginal islands in larvae.

(FlyBase Curators, 2017)

ScerGAL4^bun-GSG5961 drives mifepristone-inducible expression in adult intestinal stem cells.

(Foronda et al., 2014)

ScerGAL4^bun-GSG5961 drives mifepristone-inducible expression primarily in neurons. Weak expression is observed in 2-5 lateral cells/hemisegment in the ventral nerve cord. Strong expression is obversed in a small ventral cluster of neurons in the brain that form projections surrounding the brain midline. Moderate expression is observed in the optic lobe primodia; weak expression is observed in a few scattered cells that may be glia. In the PNS, strong expression is observed in class I dendritic arborizing neuron (type I md-da) and abdominal ventral bipolar neuron vbp (vbd); weak expression is observed in a subset of external sensory and chordotonal organs.

(Nicholson et al., 2008)

Marker for

midgut imaginal island

(Mathur et al., 2010)

Reflects expression of

bun

(Mathur et al., 2010, Nicholson et al., 2008)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Mathur et al., 2010)

Avic\GFP^2x.EGFP.UAS

(Nicholson et al., 2008)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

5961

(Cole et al., 2012.7.6, Nicholson et al., 2008)

5961 Geneswitch-Gal4

(Biteau et al., 2010)

5961-GAL4^GS

(Resnik-Docampo et al., 2021, Loza-Coll et al., 2014)

5961-GS

(Xu et al., 2025, Ureña et al., 2024, Sharma et al., 2020)

5961-Gal4

(Rera et al., 2011)

5961^GS

(Resnik-Docampo et al., 2021)

5961GS

(Ureña et al., 2024, Wang et al., 2015, Biteau et al., 2010)

5961GS Gal4

(Foronda et al., 2014)

5961GS-GAL4

(Ulgherait et al., 2020)

5961GeneSwitch-Gal4

(Fang et al., 2019)

GS5961

(Ureña et al., 2024, Martínez Corrales et al., 2020, Filer et al., 2017, Slack et al., 2015)

GSG5961

(Nicholson et al., 2008)

Pswitch AMP

(Mathur et al., 2010)

Pswitch line 5961

(Mathur et al., 2010)

Pswitch^AMP

(Mathur et al., 2010)

P{Switch1}AMP

(FlyBase, 1992-)

P{Switch2}GSG5961

(FlyBase, 1992-)

P{Switch2}bun^GSG5961

pSwitch^AMP

(Suijkerbuijk et al., 2016)

pSwitch^all

(Suijkerbuijk et al., 2016)

Name Synonyms

Secondary FlyBase IDs

FBti0129676
FBti0115009

References (21)

Report Sections

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