Amino acid replacement: Q935term.
C12536573T
Q935term | bun-PA; Q826term | bun-PF; Q826term | bun-PG; Q826term | bun-PP
Q934term
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Mutations mapped with respect to isoform -PA. The mutation was mapped to Q935. There are GLn residues at 933 and 935 in the reference sequence but not at the reported position 934.
Homozygous bunA5G3 mutant eyes show moderate defects in compensatory growth in response to tissue loss (induced by generating larval clones of the temperature-sensitive cell-lethal mutation sec5ts1 under the control of Scer\GAL4en-e16E at the restrictive temperature). The resulting eyes are smaller than wild type. No defects in cell proliferation are detected when the sec5ts1 cells are not ablated (i.e. when the flies are maintained at the permissive temperature). The proportion of mutant eye tissue seen following generation of wild type non-ablating clones is similar at 25[o]C and 30[o]C suggesting the mutation is not temperature sensitive. bunA5G3 mutant eyes show moderate growth defects when tissue is ablated during the larval stages using irradiation.
Defects in compensatory growth are also seen following sec5ts1 induced tissue loss in wing discs. Both the anterior and posterior compartment are small compared to wild type. Elevated levels of apoptosis are not seen in bunA5G3 mutant cells and mitoses are still evident, suggesting that the cells are viable and capable of proliferating.
The chromosome used in this paper also contains a mutation in stc that the authors were unable to recombine. However they exclude the possibility that the stc mutation is contributing to the phenotype based on the fact that other independent alleles of bun phenocopy the growth defects seen in this allele (data was not shown).