FlyBase Gene Report: Dmel\Obsc

General Information

Symbol

Dmel\Obsc

Species

D. melanogaster

Name

Obscurin

Annotation Symbol

CG33519

Feature Type

protein_coding_gene

FlyBase ID

FBgn0053519

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

Obscurin (Obsc) encodes a titin-like protein located at the midline of the muscle thick filaments that is needed for assembly of a symmetrical sarcomere. It is involved in myogenesis and the Hippo signalling pathway. [Date last reviewed: 2018-11-15] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Unc-89, BEST:HL01080 , obs

Key Links

Genomic Location

Cytogenetic map

60B1-60B2

Sequence location

2R:23,995,543..24,014,254 [-]

Recombination map

2-106

RefSeq locus

NT_033778 REGION:23995543..24014254

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

enables ATP binding

inferred from direct assay

(Zacharchenko et al., 2023)

enables protein binding

inferred from physical interaction with UniProtKB:Q9VCA8

(Katzemich et al., 2015)

enables protein kinase binding

inferred from physical interaction with UniProtKB:Q7KRY6

(Katzemich et al., 2015)

enables tropomyosin binding

inferred from physical interaction with UniProtKB:P06754

(Katzemich et al., 2015)

NOT enables protein kinase activity

inferred from direct assay

(Zacharchenko et al., 2023)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719

(InterPro Project Members, 2004-)

enables guanyl-nucleotide exchange factor activity

inferred from electronic annotation with InterPro:IPR000219

(InterPro Project Members, 2004-)

NOT enables protein kinase activity

inferred from key residues

(Anamika et al., 2009)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in adult somatic muscle development

inferred from mutant phenotype

(Schnorrer et al., 2010)

involved_in protein localization to M-band

inferred from mutant phenotype

(Katzemich et al., 2015)

involved_in sarcomere organization

inferred from mutant phenotype

(Katzemich et al., 2012, Schnorrer et al., 2010)

inferred from mutant phenotype

(Katzemich et al., 2015)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in M band

inferred from direct assay

(Katzemich et al., 2012, Burkart et al., 2007)

inferred from high throughput direct assay

(Sarov et al., 2016)

inferred from direct assay

(Katzemich et al., 2015)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

UNCLASSIFIED PSEUDOKINASES

Protein Family (UniProt)

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. (A8DYP0)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

UNCLASSIFIED PSEUDOKINASES -: This group comprises pseudokinases that do not classify under other groups in FlyBase.

Protein Function (UniProtKB)

Structural component of the muscle M line which is involved in assembly and organization of sarcomere (PubMed:22467859, PubMed:26251439). Required for the development and organization of indirect flight muscle sarcomeres by regulating the formation of M line and H zone and the correct assembly of thick and thin filaments in the sarcomere (PubMed:22467859, PubMed:26251439). Lacks serine/threonine-protein kinase activity (PubMed:37121260).

(UniProt, A8DYP0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Obsc for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A8DYP0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

8AK2
8AK3

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Obsc-RC

FBtr0113464

NM_001103970

13189

4218

Obsc-RD

FBtr0343283

NM_001014545

13117

4194

Obsc-RE

FBtr0343284

NM_001299874

13114

4193

Obsc-RF

FBtr0343285

NM_001299875

12265

3965

Obsc-RG

FBtr0343286

NM_001299876

10280

3310

Obsc-RH

FBtr0343287

NM_001299877

12244

3903

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Obsc-PC

FBpp0112375

475.7

4218

5.62

FBpp0309948

473.1

4194

5.71

FBpp0309949

473.0

4193

5.71

FBpp0309950

446.6

3965

5.70

FBpp0309951

374.1

3310

5.49

FBpp0309952

440.8

3903

5.63

A0A0B4LGC9

NP_001286806

AHN56601

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with myosin (PubMed:22467859). May interact (via protein kinase domain 1) with ball (PubMed:26251439). May interact (via protein kinase domain 1 or 2) with mask (PubMed:26251439). May interact (via protein kinase domain 2) with Tm1/tropomyosin-1 (PubMed:26251439).

(UniProt, A8DYP0)

Domain

The protein kinase domain 1 binds ATP with high affinity in a Mg(2+)-independent manner (PubMed:37121260). However, lacks canonical protein kinase activity, probably due to the presence of a glycine residue instead of an aspartate residue at position 3306 in the catalytic site pocket (PubMed:37121260).

The protein kinase domain 2 is predicted to be catalytically inactive.

(UniProt, A8DYP0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

8AK2
8AK3

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Obsc using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.58

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

larval somatic muscle cell

• M band

(Katzemich et al., 2012)

larval stage

larval somatic muscle cell

• M band

(Katzemich et al., 2012)

pupal stage P6

indirect flight muscle cell

Comment: 30 hr APF

(Katzemich et al., 2012)

pharate adult stage P8 -- pharate adult stage P15(ii)

indirect flight muscle cell

• M band

Comment: 48 hr APF-eclosion

(Katzemich et al., 2012)

adult stage

indirect flight muscle cell

• M band

(Burkart et al., 2007)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

organism

(Katzemich et al., 2012)

adult stage

indirect flight muscle cell

(Katzemich et al., 2012)

adult thorax

(Katzemich et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in M band

inferred from direct assay

(Katzemich et al., 2012, Burkart et al., 2007)

inferred from high throughput direct assay

(Sarov et al., 2016)

inferred from direct assay

(Katzemich et al., 2015)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Obsc in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Obsc

View exonic expression by tissue for Dmel\Obsc

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0053519

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3346201/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053519

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0053519

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053519

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33519

Flygut - An atlas of the Drosophila adult midgut

FBgn0053519

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Obsc

Transgenic constructs containing regulatory region of Obsc

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6080

(Ryder, 2004.4.26)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Obsc^GD14839

Other Phenotypes

Allele

flightless

Obsc^EY15484

flightless, with Scer\GAL4^Hk-UH3

Obsc^GD14839

flightless, with Scer\GAL4^Mef2.PR

Phenotype manifest in

Allele

H zone

Obsc^EY15484

H zone | adult stage, with Scer\GAL4^Hk-UH3

Obsc^GD14839

H zone | adult stage, with Scer\GAL4^Mef2.PR

Obsc^GD14839

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

Obsc^GD14839

indirect flight muscle cell | adult stage

Obsc^EY15484

indirect flight muscle cell | adult stage, with Scer\GAL4^Hk-UH3

Obsc^GD14839

indirect flight muscle cell | adult stage, with Scer\GAL4^Mef2.PR

Obsc^GD14839

M band

Obsc^EY15484

M band, with Scer\GAL4^Mef2.PR

Obsc^GD14839

myofibril | adult stage

Obsc^EY15484

myofibril | adult stage, with Scer\GAL4^Hk-UH3

Obsc^GD14839

myofibril | adult stage, with Scer\GAL4^Mef2.PR

Obsc^GD14839

sarcomere, with Scer\GAL4^Mef2.PR

Obsc^GD14839

sarcomere | pharate adult stage, with Scer\GAL4^Mef2.PR

Obsc^GD14839

striated muscle thin filament | adult stage

Obsc^EY15484

striated muscle thin filament | adult stage, with Scer\GAL4^Mef2.PR

Obsc^GD14839

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (142)

Hsap\SPEG

6 of 14

Yes

Hsap\HMCN1

4 of 14

Yes

Hsap\OBSCN

4 of 14

Hsap\TTN

4 of 14

Hsap\HMCN2

3 of 14

Yes

Hsap\KALRN

3 of 14

Hsap\OBSL1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\DAPK3

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (52)

Rnor\Speg

6 of 14

Yes

Rnor\Hmcn1

4 of 14

Yes

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Vwa7

1 of 14

Mus musculus (laboratory mouse) (58)

Mmus\Speg

6 of 14

Yes

Mmus\Hmcn1

5 of 14

Yes

Mmus\Hmcn2

4 of 14

Yes

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Vwa7

1 of 14

Xenopus tropicalis (Western clawed frog) (57)

Xtro\speg

3 of 13

Yes

Xtro\hmcn1

2 of 13

Yes

Xtro\hmcn2

2 of 13

Yes

Xtro\mypn

2 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\mxra5

1 of 13

Xtro\mylkl

1 of 13

Xtro\net1

1 of 13

Yes

Xtro\nrcam

1 of 13

Xtro\obscn

1 of 13

Yes

Xtro\pdgfc

1 of 13

Yes

Xtro\plekhg2

1 of 13

Xtro\plekhg4

1 of 13

Xtro\prex1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (85)

Drer\spegb

6 of 14

Yes

Drer\hmcn2

5 of 14

Yes

Drer\spega

5 of 14

Yes

Drer\hmcn1

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\dapk2a

2 of 14

Yes

Drer\dapk3

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\dapk2b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC108179342

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\si:ch73-380l3.2

1 of 14

Yes

Drer\si:ch73-380l3.3

1 of 14

Yes

Drer\si:ch73-380l3.4

1 of 14

Yes

Drer\si:dkey-24p1.1

1 of 14

Drer\si:dkey-65j6.2

1 of 14

Drer\si:dkey-240h12.4

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\vsig10l

1 of 14

Yes

Drer\vwa10.1

1 of 14

Yes

Drer\vwa10.2

1 of 14

Yes

Drer\zgc:171470

1 of 14

Drer\zgc:172106

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (68)

Cele\unc-89

7 of 14

Yes

Cele\him-4

3 of 14

Yes

Cele\cmk-1

2 of 14

Cele\dapk-1

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\F59E12.1

1 of 14

Cele\grl-1

1 of 14

Yes

Cele\grl-4

1 of 14

Yes

Cele\grl-5

1 of 14

Yes

Cele\grl-6

1 of 14

Yes

Cele\grl-12

1 of 14

Yes

Cele\grl-13

1 of 14

Yes

Cele\grl-15

1 of 14

Yes

Cele\grl-17

1 of 14

Yes

Cele\grl-21

1 of 14

Yes

Cele\grl-30

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\zig-7

1 of 14

Yes

Cele\zig-11

1 of 14

Yes

Cele\zig-12

1 of 14

Yes

Cele\zyg-1

1 of 14

Yes

Cele\zyg-8

1 of 14

Anopheles gambiae (African malaria mosquito) (74)

Agam\AgaP_AGAP005471

10 of 12

Yes

Agam\AgaP_AGAP002268

3 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

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1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (54)

Atha\CDPK1

2 of 13

Yes

Atha\CDPK6

2 of 13

Yes

Atha\CDPK19

2 of 13

Yes

Atha\CPK2

2 of 13

Yes

Atha\CPK7

2 of 13

Yes

Atha\CPK9

2 of 13

Yes

Atha\CPK14

2 of 13

Yes

Atha\CPK17

2 of 13

Yes

Atha\CPK24

2 of 13

Yes

Atha\CPK29

2 of 13

Yes

Atha\CPK32

2 of 13

Yes

Atha\CPK33

2 of 13

Yes

Atha\CPK34

2 of 13

Yes

Atha\AGP1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT5G19810

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (9)

Scer\CMK1

1 of 13

Yes

Scer\CMK2

1 of 13

Yes

Scer\IKS1

1 of 13

Yes

Scer\MEK1

1 of 13

Yes

Scer\NNK1

1 of 13

Yes

Scer\RCK1

1 of 13

Yes

Scer\RCK2

1 of 13

Yes

Scer\SKM1

1 of 13

Yes

Scer\TDA1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\cmk1

1 of 12

Yes

Spom\cmk2

1 of 12

Yes

Spom\mek1

1 of 12

Yes

Spom\nak1

1 of 12

Yes

Spom\srk1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Obsc. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (65)

sls

4 of 13

3 of 13

zormin

3 of 13

CaMKI

2 of 13

sqa

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

dpy

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Irc

1 of 13

kek6

1 of 13

klg

1 of 13

Lac

1 of 13

msn

1 of 13

Muc91C

1 of 13

nkt

1 of 13

Nrg

1 of 13

otk2

1 of 13

otk

1 of 13

PKD

1 of 13

plum

1 of 13

Ppn

1 of 13

Pura

1 of 13

Pxd

1 of 13

Pxn

1 of 13

Pxt

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

sdk

1 of 13

Strn-Mlck

1 of 13

tutl

1 of 13

Vav

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

SPEG; striated muscle enriched protein kinase

model of centronuclear myopathy 5

IEA

(FlyBase, 2019-)

HMCN1; hemicentin 1

IEA

(FlyBase, 2019-)

OBSL1; obscurin like cytoskeletal adaptor 1

model of 3-M syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SPEG; striated muscle enriched protein kinase

6 of 14

615950

MYOPATHY, CENTRONUCLEAR, 5; CNM5

centronuclear myopathy 5

HMCN1; hemicentin 1

4 of 14

608548

MACULAR DEGENERATION, AGE-RELATED, 1; ARMD1

age related macular degeneration 1

OBSCN; obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF

4 of 14

608616

RHABDOMYOLYSIS, SUSCEPTIBILITY TO, 1; RHABDO1

4 of 14

3 of 14

KALRN; kalirin RhoGEF kinase

3 of 14

604605

OBSL1; obscurin like cytoskeletal adaptor 1

3 of 14

610991

THREE M SYNDROME 2; 3M2

3-M syndrome

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Obsc - Mhc

anti bait coimmunoprecipitation, peptide massfingerprinting

(Katzemich et al., 2012)

Obsc - mask

two hybrid, pull down, anti tag western blot

(Katzemich et al., 2015)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, A8DYP0 )

Linkouts

BioGRID - A database of protein and genetic interactions.

77919

DroID - A comprehensive database of gene and protein interactions.

FBgn0053519

MIST (protein-protein) - An integrated Molecular Interaction Database

3346201

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A8DYP0

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-106

Cytogenetic map

60B1-60B2

Sequence location

2R:23,995,543..24,014,254 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60B1-60B2

Limits computationally determined from genome sequence between P{lacW}Phm^k07623&P{lacW}tsr^k05633 and P{EP}EP503

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-106

(FlyBase, 2016)

2-109.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

34292

y¹ w^*; Mi{MIC}Obsc^MI02198

21139

y¹ w^67c23; P{EPgy2}Obsc^EY15484

81168

y¹ w^*; TI{CRIMIC.TG4.1}Obsc^{CR01060-TG4.1-X}

60525

y¹ w^67c23; Mi{PT-GFSTF.0}Obsc^{MI02198-GFSTF.0}

605457

y¹ w^*; TI{CRIMIC.TG4.1}Obsc^{CR71415-TG4.1}/SM6a

34000

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00963}attP2

NIG-Fly

HMS00963

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00963}attP2

VDRC

v106267

P{KK104226}VIE-260B

v29412

w¹¹¹⁸; P{GD14839}v29412

v318326

PBac{fTRG01046.sfGFP-TVPTBF}VK00033

v342448

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01419}attP40/CyO-GFP

v342209

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01797}attP40/CyO-GFP

More stocks available...

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (35)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0053519

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0053519

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Zacharchenko et al., 2023, Katzemich et al., 2015)

monoclonal

(Loreau et al., 2023)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

BB8/351.1

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against CG30171 tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

New annotation (CG30175) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG18019 CG18020 CG18021 CG3901

(BDGP Project Members, 2000-)

Source for merge of: CG18020 CG18021

(Misra, 2001.8.10)

Source for merge of: CG18019 CG18020 CG18021

(FlyBase Genome Annotators, 2005, FlyBase, 1996-)

Source for merge of: CG18019 CG3901

(FlyBase Genome Annotators, 2002-2003)

Source for merge of: BEST:HL01080 CG30171

(FlyBase, 1996-)

Additional comments

Source for merge of CG18019 CG18020 CG18021 was a shared cDNA ( date:010720 ).

(FlyBase Genome Annotators, 2005, FlyBase, 1996-)

Annotations CG18019, CG18020, CG18021 and CG3901 merged as CG30171 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of BEST:HL01080 CG30171 was a shared cDNA ( date:030206 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Unc-89 CG33519

(Russell and Geisler, 2006.3.3)

Source for identity of: Obsc Unc-89

(Zacharchenko et al., 2023)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

BEST:HL01080

(White, 2000.2.23, White et al., 1999)

CG18019

CG18020

(Arbeitman et al., 2002)

CG18021

(Misra, 2001.8.10)

CG30171

(Katzemich et al., 2012, Burkart et al., 2007, Giot et al., 2003, Kiger et al., 2003, Vogel et al., 2003)

CG30175

CG33519

(National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Katzemich et al., 2012, Cammarato et al., 2011, Ni et al., 2010.12.1, Ellis and Carney, 2009, Keleman et al., 2009.8.5, Burkart et al., 2007, Morrison et al., 2000, FlyBase, 1996-)

CG3901

(Kashevsky et al., 2002)

CT12925

(Hynes and Zhao, 2000)

CT40322

(Hynes and Zhao, 2000)

CT40326

(Hynes and Zhao, 2000)

Obsc

(Bischoff et al., 2025, Zacharchenko et al., 2023, Gene Disruption Project members, 2018-)

Obscurin

(Rodier et al., 2025)

UNC-89

(Russell and Geisler, 2006.3.3)

Unc-89

(Rommelaere et al., 2025, Adashev et al., 2022, Bordet et al., 2021, Ostalé et al., 2021, Bataillé et al., 2020, Hassan et al., 2020, Gene Disruption Project members, 2018-, Kuleesha et al., 2016, Sarov et al., 2016, Gene Disruption Project members, 2015-, Katzemich et al., 2015, Ashwal-Fluss et al., 2014, DeSalvo et al., 2014, Kwon et al., 2013, Katzemich et al., 2012, Rodriguez et al., 2012, Wasbrough et al., 2010, Ellis and Carney, 2009, Sutter et al., 2004)

dmObsc

(Zacharchenko et al., 2023)

obs

(Katzemich et al., 2012, Burkart et al., 2007)

unc-89

(Daneshparvar et al., 2020, Spletter et al., 2018, Schnorrer et al., 2010)

Name Synonyms

Obscurin

(Trujillo et al., 2024, Loreau et al., 2023, Deng et al., 2021, Molnár et al., 2014, Sevdali et al., 2013, Katzemich et al., 2012)

PROJECTIN-like

(Morrison et al., 2000)

Unc-89

(Cammarato et al., 2011)

obscurin

(Zacharchenko et al., 2023, Daneshparvar et al., 2020, Manring et al., 2017, Katzemich et al., 2015, Katzemich et al., 2013, Katzemich et al., 2012, Miller et al., 2008, Burkart et al., 2007)

Secondary FlyBase IDs

FBgn0034952
FBgn0034953
FBgn0034954
FBgn0034955
FBgn0040499
FBgn0050171
FBgn0050175
FBtr0072198
FBpp0072107
FBpp0072108
FBtr0072200
FBpp0072109

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 77 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

3346201

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A8DYP0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A8DYP0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A8DYP0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0053519

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3346201/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053519

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053519

FlyMine - An integrated database for Drosophila genomics

FBgn0053519

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33519

MARRVEL_MODEL - MARRVEL (model organism gene)

3346201

PDB - An information portal to biological macromolecular structures

8AK2
8AK3

Linkouts

BioGRID - A database of protein and genetic interactions.

77919

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0053519

DroID - A comprehensive database of gene and protein interactions.

FBgn0053519

DRSC - Results frm RNAi screens

FBgn0053519

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

BB8/351.1

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0053519

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0053519

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33519

Flygut - An atlas of the Drosophila adult midgut

FBgn0053519

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032479

MIST (protein-protein) - An integrated Molecular Interaction Database

3346201

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A8DYP0

References (93)

The following external sites may use different assemblies or annotations than FlyBase.

-0.58

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