robo, roundabout, Robo-1, dRobo1, dRobo
Ig and Fn repeats protein - a repulsive guidance receptor on growth cones that binds to Slit - Slit binding via the Ig1 domain is essential for midline repulsion by Drosophila Robo1 but dispensable for receptor expression, localization, and regulation
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.51
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\robo1 using the Feature Mapper tool.
At stage 13 robo protein is expressed at higher levels on growth cones and filopodia in the longitudinal tracts than on the axons themselves. Very low levels are observed on the surface of commissural axons and robo-positive vessels can be seen inside the commissural axons.
robo protein has widespread and diffuse expression in the adult brain. It is expressed in all neuropil regions. The projection of clock neurons labelled by Pdf or Scer\GAL4tim.PE colocalise with domains of robo expression, whereas the cell bodies do not.
In filleted stage 17 embryos, robo1 is detected in the dendritic arborizing neurons and in the dendrites. In larval stages, expression is observed in the class I and class IV dendritic arborizing neurons and in the initial dendritic segments.
also expressed at muscle insertion sites of embryonic CNS in stage 16 embryos
robo protein is weakly expressed in the cell bodies of embryonic chordotonal neurons. It is strongly expressed in the tips of the dendrites of chordotonal neurons.
robo protein is detected weakly in the cell bodies of the dorsal cluster sensory neurons in mid stage 13 embryos just prior to axon outgrowth. Stronger robo staining is detected on outgrowing projections from these neurons through stage 15. robo protein is also detected on the projections of lch5 chordotonal neurons in stage 14. From late stage 13, robo is expressed in longitudinal muscle epidermal attachment sites.
robo protein is first observed in weak lateral stripes during germ band elongation. At the beginning of germ band retraction, it is observed in the neurectoderm. Beginning at the end of stage 12, robo protein is expressed in the growth cones which project ipsilaterally. These include aCC, pCC, MP1, dMP2 and vMP2. No expression is seen on commissural growth cones as they extend across the midline but when they turn to project longitudinally, expression dramatically increases. robo protein is expressed at high levels on all longitudinally projecting growth cones. robo protein is also expressed weakly throughout the epidermis and at a higher level at muscle attachment sites. Faint staining is observed in the commissures in stages 16 and 17.
GBrowse - Visual display of RNA-Seq signalsView Dmel\robo1 in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The lateral positioning of longitudinal axon pathways in the embryo relies primarily on differences in robo gene regulation, not on distinct combinations of the three robo proteins (robo, lea and robo3) as previously thought. However, the unique structural features of robo are required to prevent midline crossing and the unique structural features of lea are required to promote midline crossing.
Nonsense-mediated mRNA decay (NMD) down-regulates a distinct splice isoform(s) of this gene.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
robo is transiently required to stop longitudinal glia migration short of the midline, though is not responsible for restricting glial movement from the time that the glia contact the axons.
The cytoplasmic domain of the guidance receptor robo can determine the growth cones' response in vivo, mediating repulsion independently of the ectodomain and its particular ligand binding.
Four EMS induced alleles were identified in a screen for mutations affecting commissure formation in the CNS of the embryo.
The robo gene product both repels growth cones at the midline and inhibits branching throughout the neuropile by promoting filopodial retraction.
Mutations in 12 complementation groups differentially affect lateral chordotonal axon growth, fasciculation or ventral orientation. Mutations in robo, spen, sli and los cause lch axon defasciculation.
The robo gene product plays a role in the repulsive signalling system, a component of the mechanism that guides growth cones towards and away from the midline. In mutant embryos the growth cones that normally extend only on their own side of the central nervous system (CNS) now project across the midline of one of the commissures.
Mutant alleles have "fuzzy commisure" phenotype in embryonic central nervous system.