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Citation
Myat, A., Henry, P., McCabe, V., Flintoft, L., Rotin, D., Tear, G. (2002). Drosophila nedd4, a ubiquitin ligase, is recruited by commissureless to control cell surface levels of the roundabout receptor.  Neuron 35(3): 447--459.
FlyBase ID
FBrf0151451
Publication Type
Research paper
Abstract

Crossing the midline produces changes in axons such that they are no longer attracted to the midline. In Drosophila, Roundabout reaches high levels on axons once they have crossed the midline, and this prohibits recrossing. Roundabout protein levels are regulated by Commissureless. We show that Commissureless binds to and is regulated by the ubiquitin ligase DNedd4. We further show that the ability of Commissureless to regulate Roundabout protein levels requires an intact DNedd4 binding site and ubiquitin acceptor sites within the Commissureless protein. The ability of Commissureless to regulate Robo in the embryo also requires a Commissureless/DNedd4 interaction. Our results show that changes in axonal sensitivity to external cues during pathfinding across the midline makes use of ubiquitin-dependent mechanisms to regulate transmembrane protein levels.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
    Data From Reference
    Alleles (9)
    Gene Groups (1)
    Genes (4)
    Physical Interactions (8)
    Cell Lines (1)
    Experimental Tools (2)
    Transgenic Constructs (6)