FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
comm, robo1
FlyBase ID
FBig0000104422
Interaction Network
Interactions Browser links
comm network
robo1 network
Reported Interactions
FBrf0151451-6.coIP.WB
Reference
Myat et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
comm 

Comm

bait

GFP tag

robo1 

Robo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Myat et al., 2002)

Positive control.

(Myat et al., 2002)
FBrf0151451-8.coIP.WB
Reference
Myat et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
comm 

Comm

bait

Myc tag

robo1 

Robo

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Myat et al., 2002)

Binding of comm to robo1 does not require the comm PY motifs (PPCY and LPSY).

(Myat et al., 2002)

Positive control.

(Myat et al., 2002)
FBrf0206292-1.coIP.WB
Reference
Gilestro, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo1 

Robo

bait

HA tag

robo1 
robo1 
comm 

Comm

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
peri-membrane domain
sufficient binding region
aa 891-973
robo1 
peri-membrane domain
necessary binding region
aa 891-973
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gilestro, 2008)
FBrf0206292-2.coIP.WB
Reference
Gilestro, 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
robo1 

Robo

bait

HA tag

robo1 
comm 

Comm

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
peri-membrane domain
necessary binding region
aa 891-973
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Gilestro, 2008)
FBrf0206292-3.coIP.WB
Reference
Gilestro, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
comm 

Comm

bait

Myc tag

robo1 

Robo

prey

HA tag

robo1 
robo1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
peri-membrane domain
sufficient binding region
aa 891-973
robo1 
peri-membrane domain
necessary binding region
aa 891-973
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gilestro, 2008)
FBrf0262475-2.coIP.WB
Reference
Sullivan and Bashaw, 2025
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
comm 

comm

bait

HA tag

robo1 

Robo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sullivan and Bashaw, 2025)

comm-robo1-Nedd4 form a complex. Captured by 3 pairwise interactions.

(Sullivan and Bashaw, 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)