FlyBase Allele Report: Dmel\robo1[mini-TG4.1]

General Information

Symbol

Dmel\robo1^mini-TG4.1

Species

D. melanogaster

Name

FlyBase ID

FBal0385998

Feature type

allele

Associated gene

Dmel\robo1

Associated Insertion(s)

TI{CRIMIC.mTG4.1}robo1^mini-TG4.1

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Huang et al., 2022)

Mutagen

gene targeting by homologous recombination

(Huang et al., 2022)

CRISPR/Cas9

(Huang et al., 2022)

Progenitor genotype

Associated Insertion(s)

TI{CRIMIC.mTG4.1}robo1^mini-TG4.1

Cytology

Description

A TI{CRIMIC.mTG4.1} cassette has been inserted into endogenous robo1 (within a shared intron of all robo1 transcripts). The sgRNA sequence used to target the gene was: TTATAATCGGAGACAAAGCTGGG.

(Huang et al., 2022)

Allele components

Component

Use(s)

Inserted element

TI{CRIMIC.mTG4.1}

gene trap

Encoded product / tool

miniGAL4(2)

binary expression system - driver

Also carries

attP

integrase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of myoclonic-atonic epilepsy

CEA

(Huang et al., 2022)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Huang et al., 2022)

abnormal neuroanatomy | embryonic stage (with Df(2R)BSC787)

(Huang et al., 2022)

abnormal neurophysiology | adult stage (with Df(2R)BSC787)

(Huang et al., 2022)

partially lethal - majority die

(Huang et al., 2022)

partially lethal - majority die (with Df(2R)BSC787)

(Huang et al., 2022)

partially lethal - majority die (with robo1¹)

(Huang et al., 2022)

Phenotype Manifest In

connective | embryonic stage

(Huang et al., 2022)

connective | embryonic stage (with Df(2R)BSC787)

(Huang et al., 2022)

retina | adult stage (with Df(2R)BSC787)

(Huang et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

robo1^mini-TG4.1 has increased mortality during development phenotype, enhanceable by Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

NOT Enhanced by

Statement

Reference

robo1^mini-TG4.1 has partially lethal - majority die phenotype, non-enhanceable by Hsap\ROBO1^S1522L.UAS/Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

NOT suppressed by

Statement

Reference

robo1^mini-TG4.1/Df(2R)BSC787 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

robo1^mini-TG4.1/Df(2R)BSC787 has partially lethal - majority die phenotype, non-suppressible by Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

robo1^mini-TG4.1 has partially lethal - majority die phenotype, non-suppressible by Hsap\ROBO1^S1522L.UAS/Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

robo1^mini-TG4.1/Df(2R)BSC787 has retina | adult stage phenotype, non-suppressible by Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

robo1^mini-TG4.1/Df(2R)BSC787 is rescued by robo1^F388S.UAS/Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

Partially rescued by

robo1^mini-TG4.1/Df(2R)BSC787 is partially rescued by robo1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

Not rescued by

robo1^mini-TG4.1/Df(2R)BSC787 is not rescued by robo1^D413G.UAS/Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

99667

y¹ w^*; TI{CRIMIC.mTG4.1}robo1^mini-TG4.1/CyO, P{ActGFP}JMR1; PBac{UAS-robo1.H}VK00033

99668

y¹ w^*; TI{CRIMIC.mTG4.1}robo1^mini-TG4.1/CyO, P{ActGFP}JMR1; PBac{UAS-robo1.D413G}VK00033

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

robo1^T2A-GAL4

(Huang et al., 2022)

robo1^mini-TG4.1

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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