FlyBase Insertion Report: Dmel\TI{CRIMIC.mTG4.1}robo1[mini-TG4.1]

General Information

Symbol

Dmel\TI{CRIMIC.mTG4.1}robo1^mini-TG4.1

Species

D. melanogaster

Name

FlyBase ID

FBti0226603

Feature type

insertion_site

Description

Inserted Element

TI{CRIMIC.mTG4.1}

LINE ID

Affected gene(s)

robo1, Scer\GAL4

Viability / fertility

Associated allele(s)

robo1^mini-TG4.1, Scer\GAL4^{robo1-mini-TG4.1}

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

2R ( 59A2-59A3 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

59A2-59A3 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

TI{CRIMIC.mTG4.1}

(Huang et al., 2022)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

robo1

(Huang et al., 2022)

Scer\GAL4

(Huang et al., 2022)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

robo1^mini-TG4.1

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^{robo1-mini-TG4.1}

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval optic lobe | restricted

(Huang et al., 2022)

eye disc | restricted

(Huang et al., 2022)

adult stage

adult central brain intrinsic neuron | subset

(Huang et al., 2022)

adult optic lobe neuron | subset

(Huang et al., 2022)

lamina | restricted

(Huang et al., 2022)

Additional Information

Statement

Reference

In the third instar larval CNS, the robo1 reporter is expressed in a very defined subset of neurons but not in glia. It is expressed sparsely in the optic lobe and eye imaginal disc. In adults, it is expressed in numerous neurons of the central brain, optic lobe and peripheral lamina, but rarely in glia.

(Huang et al., 2022)

Marker for

Reflects expression of

robo1

(Huang et al., 2022)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration