FlyBase Allele Report: Scer\GAL4[robo1-mini-TG4.1]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Scer\GAL4^{robo1-mini-TG4.1}

General Information

Symbol

Scer\GAL4^{robo1-mini-TG4.1}

Species

S. cerevisiae

Name

FlyBase ID

FBal0386004

Feature type

allele

Associated gene

Associated Insertion(s)

TI{CRIMIC.mTG4.1}robo1^mini-TG4.1

Carried in Construct

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

gene targeting by homologous recombination

(Huang et al., 2022)

(Huang et al., 2022)

Progenitor genotype

Associated Insertion(s)

TI{CRIMIC.mTG4.1}robo1^mini-TG4.1

Cytology

Description

A TI{CRIMIC.mTG4.1} cassette has been inserted into endogenous robo1 (within a shared intron of all robo1 transcripts). The sgRNA sequence used to target the gene was: TTATAATCGGAGACAAAGCTGGG.

(Huang et al., 2022)

Allele components

Component

Use(s)

Inserted element

TI{CRIMIC.mTG4.1}

Encoded product / tool

binary expression system - driver

Also carries

integrase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval optic lobe | restricted

(Huang et al., 2022)

eye disc | restricted

(Huang et al., 2022)

adult central brain intrinsic neuron | subset

(Huang et al., 2022)

adult optic lobe neuron | subset

(Huang et al., 2022)

lamina | restricted

(Huang et al., 2022)

Additional Information

Statement

Reference

In the third instar larval CNS, the robo1 reporter is expressed in a very defined subset of neurons but not in glia. It is expressed sparsely in the optic lobe and eye imaginal disc. In adults, it is expressed in numerous neurons of the central brain, optic lobe and peripheral lamina, but rarely in glia.

(Huang et al., 2022)

Marker for

Reflects expression of

(Huang et al., 2022)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Hsap\ROBO1^UAS.cHa

(Huang et al., 2022)

abnormal neuroanatomy | embryonic stage, with robo1^F388S.UAS

(Huang et al., 2022)

abnormal neuroanatomy | embryonic stage, with robo1^UAS.cHa

(Huang et al., 2022)

abnormal neurophysiology | adult stage, with robo1^D413G.UAS

(Huang et al., 2022)

short lived, with Hsap\ROBO1^UAS.cHa

(Huang et al., 2022)

Phenotype Manifest In

connective | embryonic stage, with Hsap\ROBO1^UAS.cHa

(Huang et al., 2022)

connective | embryonic stage, with robo1^F388S.UAS

(Huang et al., 2022)

connective | embryonic stage, with robo1^UAS.cHa

(Huang et al., 2022)

embryonic/larval midline of ventral nerve cord | embryonic stage, with robo1^UAS.cHa

(Huang et al., 2022)

retina | adult stage, with robo1^D413G.UAS

(Huang et al., 2022)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1} is an enhancer of increased mortality during development phenotype of robo1^mini-TG4.1

(Huang et al., 2022)

NOT Enhancer of

Statement

Reference

Hsap\ROBO1^S1522L.UAS/Scer\GAL4^{robo1-mini-TG4.1} is a non-enhancer of partially lethal - majority die phenotype of robo1^mini-TG4.1

(Huang et al., 2022)

NOT Suppressor of

Statement

Reference

Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1} is a non-suppressor of abnormal neurophysiology | adult stage phenotype of robo1^mini-TG4.1/Df(2R)BSC787

(Huang et al., 2022)

Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1} is a non-suppressor of partially lethal - majority die phenotype of robo1^mini-TG4.1/Df(2R)BSC787

(Huang et al., 2022)

Hsap\ROBO1^S1522L.UAS/Scer\GAL4^{robo1-mini-TG4.1} is a non-suppressor of partially lethal - majority die phenotype of robo1^mini-TG4.1

(Huang et al., 2022)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1} is a non-suppressor of retina | adult stage phenotype of robo1^mini-TG4.1/Df(2R)BSC787

(Huang et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

robo1^F388S.UAS/Scer\GAL4^{robo1-mini-TG4.1} rescues robo1^mini-TG4.1/Df(2R)BSC787

(Huang et al., 2022)

Partially rescues

robo1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1} partially rescues robo1^mini-TG4.1/Df(2R)BSC787

(Huang et al., 2022)

Fails to rescue

robo1^D413G.UAS/Scer\GAL4^{robo1-mini-TG4.1} fails to rescue robo1^mini-TG4.1/Df(2R)BSC787

(Huang et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

99667

y¹ w^*; TI{CRIMIC.mTG4.1}robo1^mini-TG4.1/CyO, P{ActGFP}JMR1; PBac{UAS-robo1.H}VK00033

99668

y¹ w^*; TI{CRIMIC.mTG4.1}robo1^mini-TG4.1/CyO, P{ActGFP}JMR1; PBac{UAS-robo1.D413G}VK00033

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^{robo1-T2A-GAL4}

Scer\GAL4^{robo1-mini-TG4.1}

robo1^T2A-GAL4

(Huang et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections