FlyBase Allele Report: Dmel\robo1[GD2608]

General Information

Symbol

Dmel\robo1^GD2608

Species

D. melanogaster

Name

FlyBase ID

FBal0210481

Feature type

allele

Associated gene

Dmel\robo1

Associated Insertion(s)

Carried in Construct

P{GD2608}

Also Known As

UAS-robo1 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2608}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2608

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | female, with Scer\GAL4^ppk23.2.695

(Sato et al., 2020)

abnormal neuroanatomy | adult stage | female | somatic clone, with Scer\GAL4^NP0021

(Ito et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

neurite | adult stage | ectopic | female | somatic clone, with Scer\GAL4^NP0021

(Ito et al., 2016)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of robo1^GD2608 under the control of Scer\GAL4^ppk23.2.695 leads to induction of the midline crossing ppk23-expressing axons in females.

(Sato et al., 2020)

robo1^GD2608 knockdown MARCM clones (in a Scer\GAL4^fru-NP0021 mAL cluster) often develop a male-specific ipsilateral ectopic neurite in female brains, which was never observed in wild type female neurons; knockdown in male mAL neurons has no effect.

(Ito et al., 2016)

Adults expressing robo1^GD2608 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2608

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ppk23.2.695, robo1^GD2608 has abnormal neuroanatomy | adult stage | female phenotype, enhanceable by tei^RNAi.ex4.UAS/Scer\GAL4^ppk23.2.695

(Sato et al., 2020)

Suppressed by

Statement

Reference

Scer\GAL4^ppk23.2.695, robo1^GD2608 has abnormal neuroanatomy | female | adult stage phenotype, suppressible | partially by tei^EY11779/Scer\GAL4^ppk23.2.695

(Sato et al., 2020)

Enhancer of

Statement

Reference

robo1^GD2608/Scer\GAL4^ppk23.2.695 is an enhancer of abnormal neuroanatomy | adult stage | female phenotype of Scer\GAL4^ppk23.2.695, tei^RNAi.ex4.UAS

(Sato et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^GMR43D01/robo1^GD2608 rescues robo1^Δ1/robo1[+]

(Ito et al., 2016)

Comments

Expression of robo1^GD2608 driven by Scer\GAL4^GMR43D01 rescues precocious wing switching during courtship in robo1^Δ1/+ males.

(Ito et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2608

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

robo1^GD2608

robo^GD2608

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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