FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
robo1, RhoGAP93B
FlyBase ID
FBig0000108932
Interaction Network
Interactions Browser links
robo1 network
RhoGAP93B network
Reported Interactions
FBrf0180254-1.PD.A
Reference
Lundstrom et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
RhoGAP93B 

Vilse

bait

GST tag

robo1 

Robo

prey

[35]S label

robo1 
robo1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
robo1 
intracellular domain
sufficient binding region
aa 931-1256
robo1 
CC2 Robo domain
necessary binding region
aa 1098-1256
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lundstrom et al., 2004)
FBrf0180254-2.Y2H
Reference
Lundstrom et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
RhoGAP93B 

Vilse

bait

fused to LexA DNA-binding domain

RhoGAP93B 
RhoGAP93B 
robo1 

Robo

prey

fused to B42 activation domain

robo1 
robo1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RhoGAP93B 
WW domains
sufficient binding region
aa 1-104
RhoGAP93B 
WW domains
necessary binding region
aa 1-104
robo1 
CC2 domain
sufficient binding region
aa 1098-1122
robo1 
CC2 domain
necessary binding region
aa 1034-1122
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Lundstrom et al., 2004)

Two-hybrid system: yeast LexA-BD/B42-AD

(Lundstrom et al., 2004)
FBrf0191476-1.PD.A
Reference
Hu et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
RhoGAP93B 

CrGAP

bait

GST tag

RhoGAP93B 
robo1 

Robo

prey

[35]S label

robo1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RhoGAP93B 
WW domain
sufficient binding region
aa 22-154
robo1 
cytoplasmic domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Hu et al., 2005)
FBrf0191476-2.coIP.WB
Reference
Hu et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
robo1 

Robo

bait

HA tag

RhoGAP93B 

CrGAP

prey

FLAG tag

RhoGAP93B 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RhoGAP93B 
WW domain
sufficient binding region
aa 22-154
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hu et al., 2005)
FBrf0191476-3.coIP.WB
Reference
Hu et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
RhoGAP93B 

CrGAP

bait
robo1 

Robo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Hu et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)