lea, leak, robo-2, Robo 2, D-Robo2
Stop-codon suppression (UGA) postulated; FBrf0216884.
Gene model reviewed during 5.44
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\robo2 using the Feature Mapper tool.
robo2 protein is detected in the gonads at embryonic stage 13 and at higher levels at stage 15. It is observed at contact sites between somatic gonadal precursors as well as contact sites between germ cells and somatic gonadal precursors.
At embryonic stages 12-14, robo2 is expressed in the ectodermal domains between the chordotonal organ precursors. During chordotonal organ migration, robo2 is highly expressed in a patch of visceral mesoderm in segments T2 and T3, but is not detected in the abdominal visceral mesoderm. robo2 is strongly expressed in the dendrites of embryonic chordotonal neurons.
robo2 protein is detected in several parts of the tracheal system including the dorsal trunk, transverse connectives, dorsal branch, spiracular branches and ganglionic branches in stage 13 and 14 embryos. No robo2 protein is detected in the lateral trunk or visceral branches. There is an additional stripe of epidemal expression on the dorsal surface near the anterior segment boundaries. No axonal staining is detected in the dorsal sensory neurons or lch5 chrodotonal neurons.
GBrowse - Visual display of RNA-Seq signalsView Dmel\robo2 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: robo2 lea
Source for merge of robo2 CG14347 CG14348 CG5481 CG5574 was sequence comparison ( date:001223 ).
robo2 protein promotes midline crossing in the developing nervous system by inhibiting the activity of robo1 protein. robo2 does not promote midline crossing in the cells in which is is produced. Instead, it instructs other robo1-producing nerve cells to cross the midline.
The lateral positioning of longitudinal axon pathways in the embryo relies primarily on differences in robo gene regulation, not on distinct combinations of the three robo proteins (robo, lea and robo3) as previously thought. However, the unique structural features of robo are required to prevent midline crossing and the unique structural features of lea are required to promote midline crossing.
lea is required in the thoracic visceral mesoderm to prevent migration of neighbouring thoracic chordotonal organs during development so that they remain in a dorsal position in the thorax.