FlyBase Allele Report: Dmel\robo2[4]

General Information

Symbol

Dmel\robo2⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0121559

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Sano et al., 2005, Rajagopalan et al., 2000)

Mutagen

ethyl methanesulfonate

(Rajagopalan et al., 2000)

Progenitor genotype

robo2^EP2582

(Rajagopalan et al., 2000)

Cytology

Description

Amino acid replacements are a revision of the data reported in FBrf0132258. They are numbered relative to the 1406 amino acid residue predicted protein.

(Dickson, 2001.3.14)

Amino acid replacement: W578term.

(Dickson, 2001.3.14)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:1,391,735..1,391,735 [-]

Nucleotide change:

G1391735A

Amino acid change:

W635term | robo2-PA; W635term | robo2-PB

Reported amino acid change:

W635term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Rajagopalan et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with robo2¹)

(Nicolas and Preat, 2005)

abnormal neuroanatomy (with robo2⁵)

(Nicolas and Preat, 2005)

abnormal neuroanatomy | adult stage (with robo2⁹)

(Oliva et al., 2016)

abnormal neuroanatomy | embryonic stage (with robo2⁸)

(Spitzweck et al., 2010)

abnormal size | adult stage (with robo2⁹)

(Oliva et al., 2016)

lethal (with robo2⁸)

(Spitzweck et al., 2010)

Phenotype Manifest In

adult mushroom body (with robo2¹)

(Nicolas and Preat, 2005)

adult mushroom body (with robo2⁵)

(Nicolas and Preat, 2005)

adult s-LNv neuron | adult stage (with robo2⁹)

(Oliva et al., 2016)

axon | adult stage (with robo2⁹)

(Oliva et al., 2016)

ellipsoid body (with robo2¹)

(Nicolas and Preat, 2005)

ellipsoid body (with robo2⁵)

(Nicolas and Preat, 2005)

fan-shaped body (with robo2¹)

(Nicolas and Preat, 2005)

fan-shaped body (with robo2⁵)

(Nicolas and Preat, 2005)

ganglionic tracheal branch primordium

(Englund et al., 2002)

ganglionic tracheal branch primordium (with robo2⁸)

(Englund et al., 2002)

germline cell | embryonic stage (with robo2⁸)

(Weyers et al., 2011)

gonad | embryonic stage (with robo2⁸)

(Weyers et al., 2011)

gonadal sheath proper primordium | embryonic stage (with robo2⁸)

(Weyers et al., 2011)

intermediate longitudinal fascicle (with robo2⁸)

(Spitzweck et al., 2010)

larval longitudinal connective (with robo2¹)

(Rajagopalan et al., 2000, Rajagopalan et al., 2000)

larval longitudinal connective (with robo2⁸)

(Spitzweck et al., 2010)

lateral longitudinal fascicle (with robo2⁸)

(Spitzweck et al., 2010)

medial lobe of adult mushroom body (with robo2¹)

(Nicolas and Preat, 2005)

medial lobe of adult mushroom body (with robo2⁵)

(Nicolas and Preat, 2005)

vertical lobe of adult mushroom body (with robo2¹)

(Nicolas and Preat, 2005)

vertical lobe of adult mushroom body (with robo2⁵)

(Nicolas and Preat, 2005)

Detailed Description

Statement

Reference

The adult sLNv neurons of robo2⁴/robo2⁹ transheterozygotes show several morphological defects of variable penetrance, including axon projection defects and mis-placed cell body, as compared to controls; however, the few mutant axons that do reach the calyx display normal length of the terminal branches.

(Oliva et al., 2016)

lea^robo2-8/lea^robo2-4 stage 13 embryos have gonads with unfused somatic gonadal precursor (SGP) clusters. By stage 15, SGP clusters fuse but gonads then fail to compact properly. Mutants also show germ cell ensheathment defects.

(Weyers et al., 2011)

Fas2-positive longitudinal axons show a number of defects in lea^robo2-4/lea^robo2-8 stage 16 embryos; Fas2-positive axons extend across or along the midline in 23.7% of segments, intermediate Fas2-positive fascicles are fused or broken in 10.2% of hemisegments and lateral Fas2-positive fascicles are fused or broken in 29.9% of hemisegments.

(Spitzweck et al., 2010)

lea^robo2-4/lea^robo2-5 and lea^robo2-1/lea^robo2-4 have defects in the ellipsoid body and fan-shaped body.

12% of mushroom bodies lack dorsal projections in lea^robo2-4/lea^robo2-5 animals, and 8% have a partial median projection. In addition, the the diameter of peduncle is larger than in wild-type animals.

34% of mushroom bodies lack dorsal projections in lea^robo2-4/lea^robo2-1 animals, and 18% have a partial median projection. The median lobes are positioned more posteriorly than in wild type. In addition, the the diameter of peduncle is larger than in wild-type animals.

(Nicolas and Preat, 2005)

6% of ganglionic branches cross the midline in mutant embryos. These ganglionic branches do not extend to the contralateral longitudinal connectives but linger around the midline instead. Ganglionic branches often stall outside the central nervous system in these embryos. 6% of ganglionic branches cross the midline in lea^robo2-4/lea^robo2-8 embryos and 15% of ganglionic branches fail to enter the central nervous system.

(Englund et al., 2002)

The lateral Fas2-expressing fascicle often merges with the intermediate fascicle in lea^robo2-1/lea^robo2-4 mutant embryos. Midline crossing defects also occur. 7.9% of embryos show fusion between the medial and intermediate fascicles, 17.1% show fusion between the lateral and and intermediate fascicles and 13.4% show breaks in the lateral fascicle.

(Rajagopalan et al., 2000)

In lea^robo2-1/lea^robo2-4 mutants about a quarter of Fas2 positive axons in the ventral nerve cord are misrouted. These form bundles of varying thickness.

(Rajagopalan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

robo1^unspecified, robo2⁴ has abnormal neuroanatomy phenotype, enhanceable by Abl²/Abl[+]

(Wills et al., 2002)

robo1^unspecified, robo2⁴ has abnormal neuroanatomy phenotype, enhanceable by Abl⁴/Abl[+]

(Wills et al., 2002)

NOT Suppressor of

Statement

Reference

robo2⁴/robo2⁴ is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav.PLu, unc-5^{UAS.Tag:HA,Tag:SS(wg)}

(Keleman and Dickson, 2001)

Other

Statement

Reference

Df(1)NetAB^Δ, robo2⁴ has abnormal neuroanatomy | embryonic stage phenotype

(Spitzweck et al., 2010)

robo1^unspecified, robo2⁴ has abnormal neuroanatomy phenotype

(Wills et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

robo1^unspecified, robo2⁴ has presumptive embryonic/larval central nervous system phenotype, enhanceable by Abl²/Abl[+]

(Wills et al., 2002)

robo1^unspecified, robo2⁴ has presumptive embryonic/larval central nervous system phenotype, enhanceable by Abl⁴/Abl[+]

(Wills et al., 2002)

NOT Enhanced by

Statement

Reference

robo2⁴/robo2⁸ has gonad | embryonic stage phenotype, non-enhanceable by robo1²/robo1¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has gonadal sheath proper primordium | embryonic stage phenotype, non-enhanceable by robo1²/robo1¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has germline cell | embryonic stage phenotype, non-enhanceable by robo1²/robo1¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has gonad | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has gonadal sheath proper primordium | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has germline cell | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

Suppressed by

Statement

Reference

robo2⁴/robo2⁸ has ganglionic tracheal branch primordium phenotype, suppressible | partially by robo1^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^bs.Term

(Englund et al., 2002)

NOT Enhancer of

Statement

Reference

robo2⁴/robo2⁸ is a non-enhancer of gonad | embryonic stage phenotype of Df(2L)Exel7006/robo3¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ is a non-enhancer of germline cell | embryonic stage phenotype of Df(2L)Exel7006/robo3¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ is a non-enhancer of germline cell | embryonic stage phenotype of robo1²/robo1¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ is a non-enhancer of gonad | embryonic stage phenotype of robo1²/robo1¹

(Weyers et al., 2011)

NOT Suppressor of

Statement

Reference

robo2⁴/robo2⁴ is a non-suppressor of larval anterior commissure phenotype of Scer\GAL4^elav.PLu, unc-5^{UAS.Tag:HA,Tag:SS(wg)}

(Keleman and Dickson, 2001)

robo2⁴/robo2⁴ is a non-suppressor of larval posterior commissure phenotype of Scer\GAL4^elav.PLu, unc-5^{UAS.Tag:HA,Tag:SS(wg)}

(Keleman and Dickson, 2001)

Other

Statement

Reference

Df(1)NetAB^Δ, robo2⁴ has larval anterior commissure phenotype

(Spitzweck et al., 2010)

Df(1)NetAB^Δ, robo2⁴ has larval posterior commissure phenotype

(Spitzweck et al., 2010)

robo1^unspecified, robo2⁴ has presumptive embryonic/larval central nervous system phenotype

(Wills et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Stage 16-17 Df(1)NetAB^Δ ; lea^robo2-4 double mutant embryos almost completely lack commissures; 70.7% of anterior commissures and 87.1% of posterior commissures are absent, while 27.6% of anterior commissures and 12.9% of posterior commissures are thin.

(Spitzweck et al., 2010)

Expression of robo^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^bs.Term partially rescues the failure of ganglionic branches to enter the central nervous system that is seen in lea^robo2-4/lea^robo2-8 embryos and the weak midline crossing phenotype of the ganglionic branches seen in these embryos is also rescued.

(Englund et al., 2002)

capt¹⁰ lea^robo2-4 double heterozygotes show little if any midline crossing defects in the embryonic central nervous system. The frequency of ectopic crossing of the midline by axons in the central nervous system seen in robo^unspecified lea^robo2-4 embryos is increased by Abl²/+ or Abl⁴/+.

(Wills et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

robo2⁴ is rescued by robo2^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^bs.Term

(Englund et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66884

y¹ w^*; robo2⁴/CyO, P{en1}wg^en11

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

lea^robo2-4

robo2⁴

(Oliva et al., 2016, Weyers et al., 2011, Spitzweck et al., 2010, Zmojdzian et al., 2008, Nicolas and Preat, 2005, Sano et al., 2005, Orgogozo et al., 2004, Englund et al., 2002, Wills et al., 2002, Dickson, 2001.3.14, Keleman and Dickson, 2001, Rajagopalan et al., 2000, Rajagopalan et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (14)

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