FlyBase Allele Report: Dmel\robo2[RNAi.UAS]

General Information

Symbol

Dmel\robo2^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0179063

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

Carried in Construct

P{UAS-robo2.RNAi}

Also Known As

UAS-robo2-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Tayler et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Tayler et al., 2004)

Mutagen

in vitro construct

(Tayler et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-robo2.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of a robo2 RNAi construct.

(Tayler et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

robo2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage, with Scer\GAL4^Mef2.247

(Raza and Jacobs, 2016)

abnormal neuroanatomy | adult stage | male, with Scer\GAL4^Poxn.14

(Mellert et al., 2010)

Phenotype Manifest In

embryonic heart cardioblast, with Scer\GAL4^Mef2.247

(Raza and Jacobs, 2016)

filopodium | embryonic stage, with Scer\GAL4^Mef2.247

(Raza and Jacobs, 2016)

lamellipodium | embryonic stage, with Scer\GAL4^Mef2.247

(Raza and Jacobs, 2016)

prothoracic leg taste bristle chemosensory neuron | male, with Scer\GAL4^Poxn.14

(Mellert et al., 2010)

Detailed Description

Statement

Reference

robo2^{dsRNA.Scer\UAS} knockdown MARCM clones (in a female Scer\GAL4^fru-NP0021 mAL cluster) do not develop a male-specific ipsilateral ectopic neurite (as seen in robo1^GD2608 knockdown clones).

(Ito et al., 2016)

The expression of robo2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Pdf.PU does not significantly affect the length of sLNv axons compared to controls.

(Oliva et al., 2016)

The expression of robo2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mef2.247 leads to significant decreases in migration velocity, as well as in filopodial and lamellopodial extensions in embryonic heart cardioblasts, as compared to controls.

(Raza and Jacobs, 2016)

Females expressing lea^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Poxn.14 do not show midline crossing by foreleg gustatory receptor neuron (GRN) axons (this phenotype is the same as in wild-type females). Males expressing lea^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Poxn.14 show reduced midline crossing by foreleg GRNs compared to that seen in control males.

(Mellert et al., 2010)

Expression of a single copy of lea^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^sca-375.1 has no effect on visual system development.

(Tayler et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^Pdf.PU, robo2^RNAi.UAS, robo3^RNAi.UAS has abnormal neuroanatomy | adult stage phenotype

(Oliva et al., 2016)

Scer\GAL4^Pdf.PU, robo2^RNAi.UAS, robo3^RNAi.UAS has abnormal size | adult stage phenotype

(Oliva et al., 2016)

Scer\GAL4^αTub84B.PL, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has abnormal neuroanatomy phenotype

(Tayler et al., 2004)

Scer\GAL4^sca-375.1, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has abnormal neuroanatomy phenotype

(Tayler et al., 2004)

Scer\GAL4^elav-C155, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has abnormal neuroanatomy phenotype

(Tayler et al., 2004)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^Pdf.PU, robo2^RNAi.UAS, robo3^RNAi.UAS has axon | adult stage phenotype

(Oliva et al., 2016)

Scer\GAL4^Pdf.PU, robo2^RNAi.UAS, robo3^RNAi.UAS has adult s-LNv neuron | adult stage phenotype

(Oliva et al., 2016)

Scer\GAL4^Poxn.14, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has prothoracic leg taste bristle chemosensory neuron | male phenotype

(Mellert et al., 2010)

Scer\GAL4^αTub84B.PL, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has eye photoreceptor cell & axon phenotype

(Tayler et al., 2004)

Scer\GAL4^sca-375.1, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has lamina anlage phenotype

(Tayler et al., 2004)

Scer\GAL4^sca-375.1, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has eye photoreceptor cell & axon phenotype

(Tayler et al., 2004)

Scer\GAL4^elav-C155, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has lamina anlage phenotype

(Tayler et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of robo2^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS}, but not of robo2^{dsRNA.Scer\UAS} and robo1^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^Pdf.PU results in adult sLNv neurons exhibiting significantly longer axon projections compared to controls.

(Oliva et al., 2016)

Males and females simultaneously co-expressing robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Poxn.14 show severe disruption of foreleg gustatory receptor neuron axon morphology, with axons collapsing onto the midline.

(Mellert et al., 2010)

Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 results in defects in visual system development; distal cell neurons invade the developing lamina. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-375.1 results in distal cell neurons invading the developing lamina. The distal cell neurons and lamina glia are intermingled in these animals. In addition defects in photoreceptor axon targeting are seen, with the regions of mistargeting correlating with the regions where the distal cell neurons enter the lamina. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL results in many photoreceptor axons extending through the lamina and too many photoreceptor axons entering the medulla. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF does not result in any defects in photoreceptor axon targeting.

(Tayler et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9286

w^*; sna^Sco/CyO, P{ActGFP}JMR1; P{UAS-robo2.RNAi}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

lea^{dsRNA.Scer\UAS}

robo2^RNAi.UAS

robo2^{dsRNA.Scer\UAS}

robo2^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (10)

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