FlyBase Allele Report: Dmel\robo2[5]

General Information

Symbol

Dmel\robo2⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0121558

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Rajagopalan et al., 2000)

Mutagen

ethyl methanesulfonate

(Rajagopalan et al., 2000)

Progenitor genotype

robo2^EP2582

(Rajagopalan et al., 2000)

Cytology

Description

Amino acid replacements are a revision of the data reported in FBrf0132258. They are numbered relative to the 1406 amino acid residue predicted protein.

(Dickson, 2001.3.14)

Amino acid replacement: W666term.

(Dickson, 2001.3.14)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:1,391,393..1,391,393 [-]

Nucleotide change:

G1391393A

Amino acid change:

W723term | robo2-PA; W723term | robo2-PB

Reported amino acid change:

W723term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Rajagopalan et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with robo2¹)

(Nicolas and Preat, 2005)

abnormal neuroanatomy (with robo2⁴)

(Nicolas and Preat, 2005)

Phenotype Manifest In

adult mushroom body (with robo2¹)

(Nicolas and Preat, 2005)

adult mushroom body (with robo2⁴)

(Nicolas and Preat, 2005)

ellipsoid body (with robo2¹)

(Nicolas and Preat, 2005)

ellipsoid body (with robo2⁴)

(Nicolas and Preat, 2005)

fan-shaped body (with robo2¹)

(Nicolas and Preat, 2005)

fan-shaped body (with robo2⁴)

(Nicolas and Preat, 2005)

medial lobe of adult mushroom body (with robo2¹)

(Nicolas and Preat, 2005)

medial lobe of adult mushroom body (with robo2⁴)

(Nicolas and Preat, 2005)

vertical lobe of adult mushroom body (with robo2¹)

(Nicolas and Preat, 2005)

vertical lobe of adult mushroom body (with robo2⁴)

(Nicolas and Preat, 2005)

Detailed Description

Statement

Reference

lea^robo2-4/lea^robo2-5 and lea^robo2-1/lea^robo2-5 have defects in the ellipsoid body and fan-shaped body.

16% of mushroom bodies lack dorsal projections in lea^robo2-1/lea^robo2-5 animals, and 32% have a partial median projection. In addition, the diameter of the peduncle is larger than in wild-type animals.

12% of mushroom bodies lack dorsal projections in lea^robo2-4/lea^robo2-5 animals, and 8% have a partial median projection. In addition, the the diameter of peduncle is larger than in wild-type animals.

(Nicolas and Preat, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

robo1^unspecified, robo2⁵ has abnormal neuroanatomy phenotype, enhanceable by Abl²/Abl[+]

(Wills et al., 2002)

Other

Statement

Reference

robo1^unspecified, robo2⁵ has abnormal neuroanatomy phenotype

(Wills et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

robo1^unspecified, robo2⁵ has presumptive embryonic/larval central nervous system phenotype, enhanceable by Abl²/Abl[+]

(Wills et al., 2002)

robo2⁵ has larval DA1 motor neuron phenotype, enhanceable by robo1¹

(Rajagopalan et al., 2000)

robo2⁵ has larval muscle system phenotype, enhanceable by robo1¹

(Rajagopalan et al., 2000)

robo2⁵ has pCC neuron phenotype, enhanceable by robo1¹

(Rajagopalan et al., 2000)

NOT suppressed by

Statement

Reference

robo1¹, robo2⁵ has larval ventral nerve cord phenotype, non-suppressible by comm¹

(Rajagopalan et al., 2000)

Enhancer of

Statement

Reference

robo2⁵ is an enhancer of larval DA1 motor neuron phenotype of robo1¹

(Rajagopalan et al., 2000)

robo2⁵ is an enhancer of larval muscle system phenotype of robo1¹

(Rajagopalan et al., 2000)

robo2⁵ is an enhancer of pCC neuron phenotype of robo1¹

(Rajagopalan et al., 2000)

NOT Enhancer of

Statement

Reference

robo2⁵ is a non-enhancer of larval ventral nerve cord commissure phenotype of comm¹

(Rajagopalan et al., 2000)

NOT Suppressor of

Statement

Reference

robo2⁵ is a non-suppressor of larval ventral nerve cord commissure phenotype of comm¹

(Rajagopalan et al., 2000)

Other

Statement

Reference

robo1^unspecified, robo2⁵ has presumptive embryonic/larval central nervous system phenotype

(Wills et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of ectopic crossing of the midline by axons in the central nervous system seen in robo^unspecified lea^robo2-5 embryos is increased by Abl²/+.

(Wills et al., 2002)

robo¹/lea^robo2-5 embryos show an enhancement of the embryonic nervous system phenotype seen in these embryos. The pCC axon fasciculates with its contralateral homologue to extend anteriorly right along the midline. The aCC axons also frequently cross the midline in these embryos. By stage 16, all axons have joined the single midline bundle. Double mutants also exhibit a embryonic muscle phenotype. Ventral muscles normally attach to the epidermis beneath the lateral central nervous system (CNS). In robo¹/lea^robo2-5 mutants, these muscles frequently extend across the dorsal surface of the CNS. Some muscles still attach to beneath the CNS though often somewhat close to the midline than usual. The addition of lea^robo2-5 to comm¹ embryos has no effect on the number of commissures crossing the midline in the ventral nerve cords in these mutants. The addition of comm¹ to robo¹, lea^robo2-5 embryos has no effect on the ventral nerve cord phenotype.

(Rajagopalan et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

lea^robo2-5

robo2⁵

(Nicolas and Preat, 2005, Englund et al., 2002, Wills et al., 2002, Dickson, 2001.3.14, Rajagopalan et al., 2000, Rajagopalan et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (7)

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