FlyBase Allele Report: Dmel\robo2[8]

General Information

Symbol

Dmel\robo2⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0121555

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Sano et al., 2005, Rajagopalan et al., 2000)

Mutagen

ethyl methanesulfonate

(Rajagopalan et al., 2000)

Progenitor genotype

robo2^EP2582

(Rajagopalan et al., 2000)

Cytology

Description

Amino acid replacements are a revision of the data reported in FBrf0132258. They are numbered relative to the 1406 amino acid residue predicted protein.

(Dickson, 2001.3.14)

Amino acid replacement: R845term.

(Dickson, 2001.3.14)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:1,387,324..1,387,324 [-]

Nucleotide change:

C1387324T

Amino acid change:

R902term | robo2-PA; R902term | robo2-PB

Reported amino acid change:

R902term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Rajagopalan et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Kinrade and Hidalgo, 2004, Parsons et al., 2003)

abnormal neuroanatomy | embryonic stage (with robo2⁴)

(Spitzweck et al., 2010)

lethal (with robo2⁴)

(Spitzweck et al., 2010)

Phenotype Manifest In

dorsal tracheal branch primordium

(Englund et al., 2002)

dorsal vessel wall cell

(Qian et al., 2005)

embryonic longitudinal glial cell

(Kinrade and Hidalgo, 2004)

fascicle

(Kinrade and Hidalgo, 2004)

ganglionic tracheal branch primordium (with robo2⁴)

(Englund et al., 2002)

germline cell | embryonic stage (with robo2⁴)

(Weyers et al., 2011)

germline cell | embryonic stage (with robo2^lea-2)

(Weyers et al., 2011)

gonad | embryonic stage (with robo2⁴)

(Weyers et al., 2011)

gonad | embryonic stage (with robo2^lea-2)

(Weyers et al., 2011)

gonadal sheath proper primordium | embryonic stage (with robo2⁴)

(Weyers et al., 2011)

gonadal sheath proper primordium | embryonic stage (with robo2^lea-2)

(Weyers et al., 2011)

intermediate longitudinal fascicle (with robo2⁴)

(Spitzweck et al., 2010)

larval abdominal lch5 neuron

(Parsons et al., 2003)

larval longitudinal connective

(Rajagopalan et al., 2000, Rajagopalan et al., 2000)

larval longitudinal connective (with robo2⁴)

(Spitzweck et al., 2010)

lateral longitudinal fascicle (with robo2⁴)

(Spitzweck et al., 2010)

Detailed Description

Statement

Reference

lea^robo2-8/lea² stage 13 embryos have gonads with unfused somatic gonadal precursor (SGP) clusters. By stage 15, SGP clusters fuse but gonads then fail to compact properly. Mutants also show germ cell ensheathment defects.

lea^robo2-8/lea^robo2-4 stage 13 embryos have gonads with unfused somatic gonadal precursor (SGP) clusters. By stage 15, SGP clusters fuse but gonads then fail to compact properly. Mutants also show germ cell ensheathment defects.

(Weyers et al., 2011)

Fas2-positive longitudinal axons show a number of defects in lea^robo2-4/lea^robo2-8 stage 16 embryos; Fas2-positive axons extend across or along the midline in 23.7% of segments, intermediate Fas2-positive fascicles are fused or broken in 10.2% of hemisegments and lateral Fas2-positive fascicles are fused or broken in 29.9% of hemisegments.

(Spitzweck et al., 2010)

There are no significant defects in abdominal cluster dendritic branching in animals mutant for lea^robo2-8.

The dendrite morphology of class I and class IV neurons is normal in lea^robo2-8 single cell mutant clones.

(Dimitrova et al., 2008)

Subtle myocardial cell alignment defects are observed in lea^robo2-8 stage 16 embryos.

(Qian et al., 2005)

In embryonic stage 16 lea^robo2-8 mutants, axons defasciculate and longitudinal glia are disorganised, although the phenotype is rather weak (26% penetrance) and the glia do not migrate abnormally close to the midline.

(Kinrade and Hidalgo, 2004)

The lch5 axons are stalled, most frequently before turning point TP1 but sometimes between turning points TP1 and TP2, in 1% of hemisegments in mutant embryos. Stalling is also seen in dorsal cluster axons in about 1% of thoracic and abdominal hemisegments, but the most common defect in the dorsal cluster axons is projection in aberrant directions in 5-7% of hemisegments.

(Parsons et al., 2003)

6% of ganglionic branches cross the midline in lea^robo2-4/lea^robo2-8 embryos and 15% of ganglionic branches fail to enter the central nervous system. 18% of dorsal tracheal branches are stalled or missing and 30% of the remaining branches fail to fuse over the heart.

(Englund et al., 2002)

Homozygous embryos show defects in the Fas2-expressing fascicles. 8.5% of embryos show fusion between the medial and intermediate fascicles, 23.0% show fusion between the lateral and intermediate fascicle, 4.7% show breaks in the intermediate fascicle and 15.2% show breaks in the lateral fascicle.

(Rajagopalan et al., 2000)

In robo⁸ homozygous mutants about a quarter of Fas2 positive axons in the ventral nerve cord are misrouted. These form bundles of varying thickness.

(Rajagopalan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

robo1¹, lea[+]/robo2⁸ has abnormal neuroanatomy | recessive phenotype, enhanceable by capt^k01217/capt[+]

(Wills et al., 2002)

Enhancer of

Statement

Reference

robo2⁸ is an enhancer of abnormal neuroanatomy phenotype of chb⁴, robo1¹

(Lee et al., 2004)

Other

Statement

Reference

robo1¹, robo2⁸ has abnormal neuroanatomy phenotype

(Lee et al., 2004)

robo1¹, lea[+]/robo2⁸ has abnormal neuroanatomy phenotype

(Lee et al., 2004)

robo1¹, lea[+]/robo2⁸ has abnormal neuroanatomy | recessive phenotype

(Wills et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

robo2⁸ has dorsal vessel wall cell phenotype, enhanceable by robo1¹

(Qian et al., 2005)

robo1¹, lea[+]/robo2⁸ has presumptive embryonic/larval central nervous system phenotype, enhanceable by capt^k01217/capt[+]

(Wills et al., 2002)

NOT Enhanced by

Statement

Reference

robo2⁴/robo2⁸ has gonad | embryonic stage phenotype, non-enhanceable by robo1²/robo1¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has gonadal sheath proper primordium | embryonic stage phenotype, non-enhanceable by robo1²/robo1¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has germline cell | embryonic stage phenotype, non-enhanceable by robo1²/robo1¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has gonad | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has gonadal sheath proper primordium | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ has germline cell | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

Suppressed by

Statement

Reference

robo2⁴/robo2⁸ has ganglionic tracheal branch primordium phenotype, suppressible | partially by robo1^{UAS.Tag:HA,Tag:SS(wg)}/Scer\GAL4^bs.Term

(Englund et al., 2002)

Enhancer of

Statement

Reference

robo2⁸ is an enhancer of dorsal vessel wall cell phenotype of robo1¹

(Qian et al., 2005)

robo2⁸ is an enhancer of embryonic/larval dorsal tracheal branch phenotype of stumps^ems7

(Zhu et al., 2005)

robo2⁸ is an enhancer of larval neuron phenotype of chb⁴, robo1¹

(Lee et al., 2004)

NOT Enhancer of

Statement

Reference

robo2⁴/robo2⁸ is a non-enhancer of gonad | embryonic stage phenotype of Df(2L)Exel7006/robo3¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ is a non-enhancer of gonad | embryonic stage phenotype of robo1²/robo1¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ is a non-enhancer of germline cell | embryonic stage phenotype of robo1²/robo1¹

(Weyers et al., 2011)

robo2⁴/robo2⁸ is a non-enhancer of germline cell | embryonic stage phenotype of Df(2L)Exel7006/robo3¹

(Weyers et al., 2011)

robo2⁸ is a non-enhancer of embryonic abdominal dorsal sensory organ precursor cluster phenotype of robo1¹

(Dimitrova et al., 2008)

robo2⁸ is a non-enhancer of dendrite phenotype of robo1¹

(Dimitrova et al., 2008)

NOT Suppressor of

Statement

Reference

lea[+]/robo2⁸ is a non-suppressor of adult dorsal tracheal branch | third instar larval stage phenotype of Scer\GAL4^btl.PS, Sdc^RNAi.UAS.cSa

(Schulz et al., 2011)

Other

Statement

Reference

robo1¹, robo2⁸ has larval neuron phenotype

(Lee et al., 2004)

robo1¹, lea[+]/robo2⁸ has larval neuron phenotype

(Lee et al., 2004)

robo1¹, lea[+]/robo2⁸ has presumptive embryonic/larval central nervous system phenotype

(Wills et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

lea^robo2-8/+ fails to suppress the dorsal branch fusion phenotype seen when Sdc^{dsRNA.Scer\UAS.cSa} is expressed under the control of Scer\GAL4^btl.PS.

(Schulz et al., 2011)

Many of the dorsal abdominal clusters in robo¹/lea^robo2-8 mutant animals have branches that exceed the normal level of extension at approximately 21 and 22 hours after egg laying, but this phenotype is not significantly greater than the defects observed in robo¹ mutants.

(Dimitrova et al., 2008)

robo¹, lea^robo2-8 double mutants show severe myocardial cell misalignment including gaps, intercalation, and double rows.

(Qian et al., 2005)

lea^robo2-8 enhances the defects in dorsal branch formation seen in stumps^ems7 embryos; in the double mutant 71% are missing and 8% are stalled.

(Zhu et al., 2005)

Heterozygosity for lea^robo2-8 enhances the frequency of axons ectopically crossing the midline that is seen in robo¹ chb⁴ double heterozygous embryos. robo¹ lea^robo2-8 double heterozygous embryos have axons ectopically crossing the midline.

(Lee et al., 2004)

robo¹ lea^robo2-8 double mutant embryos show misprojection of lch5 axons to the v'ch1 axon pathway in 11% of hemisegments (a similar frequency to robo¹ single mutants). Stalling of the lch5 axons prior to turning point TP2, a characteristic of the lea^robo2-8 single mutant, is also seen in double mutant embryos, although at a higher frequency (6% of hemisegments). The dorsal cluster misprojection phenotype seen in lea^robo2-8 single mutant embryos is not seen in the robo¹ lea^robo2-8 double mutant embryos; in the double mutant embryos the dorsal cluster axons follow a normal trajectory to the lateral region, although in 7% of hemisegments they grow abnormally from this point, either projecting along the v'ch1 route or growing intersegmentally. The lch5 cell bodies are located dorsal to their normal position in 11% of hemisegments and are aberrantly oriented in 3% of hemisegments of robo¹ lea^robo2-8 double mutant embryos. Missing or aberrantly positioned spiracular branches and failure of motor axons to extend as far as the sensory neuron cluster are occasionally seen.

(Parsons et al., 2003)

Expression of robo^{Scer\UAS.T:Ivir\HA1,T:wg} under the control of Scer\GAL4^bs.Term partially rescues the failure of ganglionic branches to enter the central nervous system that is seen in lea^robo2-4/lea^robo2-8 embryos and the weak midline crossing phenotype of the ganglionic branches seen in these embryos is also rescued. Expression of sli^Scer\UAS.cKa under the control of Scer\GAL4^twi.PG in a lea^robo2-8 background does not result in any new tracheal branches growing from trunk metameres T2 to T9.

(Englund et al., 2002)

One copy of capt^k01217 enhances the frequency of midline crossing defects seen in robo¹ lea^robo2-8 double mutant heterozygotes.

(Wills et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

lea^robo2-8

robo2⁸

(Schulz et al., 2011, Weyers et al., 2011, Spitzweck et al., 2010, Dimitrova et al., 2008, Sano et al., 2005, Zhu et al., 2005, Johnson et al., 2004, Kinrade and Hidalgo, 2004, Lee et al., 2004, Parsons et al., 2003, Englund et al., 2002, Wills et al., 2002, Dickson, 2001.3.14, Rajagopalan et al., 2000, Rajagopalan et al., 2000)

robo^D28

(Qian et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (17)

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