FlyBase Allele Report: Dmel\Atg16[d67]

General Information

Symbol

Dmel\Atg16^d67

Species

D. melanogaster

Name

FlyBase ID

FBal0325052

Feature type

allele

Associated gene

Dmel\Atg16

Associated Insertion(s)

Carried in Construct

Also Known As

Atg16^Δ67

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Varga et al., 2016)

Mutagen

P-element activity

(Varga et al., 2016)

Progenitor genotype

P{EP}Atg16^G5082

(Juhasz, 2017.6.13, Varga et al., 2016)

Cytology

Description

Imprecise excision of P{EP}Atg16^G5082, resulting in a 7121bp deletion extending from the original insertion site (revision of data reported in FBrf0235789).

(Juhasz, 2017.6.13)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:29,827,929..29,835,049 [-]

Comment:

Inferred boundaries of a 7121 bp deletion resulting from the imprecise excision of P{EP}Atg16^G5082 that extends from the insertion site into Atg16.

(Juhasz, 2017.6.13)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of inflammatory bowel disease 10

CEC

(Nagy et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of inflammatory bowel disease 10

is ameliorated by Atg16^{ΔAutD+Linker.UAS}

(Nagy et al., 2017)

is ameliorated by robo2^EP2582

(Nagy et al., 2017)

is ameliorated by sli^EY10695

(Nagy et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Authors state that "among our mutants, Atg16^d67 is the best to model [inflammatory bowel disease] in flies". "inflammatory bowel disease 10" is associated with human ortholog ATG12L1.

(Nagy et al., 2017)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The adult gut of Atg16^d67 homozygotes show severe morphological changes, including significant increases in length and diameter, but no changes in the peritrophic membrane thickness, as compared to controls; the epithelium is thicker and shows a significant increase in the proportion of pre-enteroendocrine cel

ls and a reciprocal significant decrease in the proportion of mature enteroendocrine cells, associated with a significant delay in pre-enteroendocrine cell maturation and a significant increase in the overall number of Prospero-positive precursor/mature enteroendocrine cells, but shows no changes in the thickness of the peritrophic membrane, in the proportion of mitotic or apoptotic cells or in cell adhesion, as compared to controls.

Atg16^d67 homozygous adults show a severe decrease in lifespan and a significant increase in the gut bacteria burden upon oral infection with Pseudomonas aeruginosa or Enterococcus faecalis, as compared to controls; in the absence of infection, these individuals show a significant increase in the overall gut bacteria burden (namely Lactobacilli and Enterobacteriaceae, but not Acetobacteriaceae), as compared to controls. These mutants do not show significant changes in food uptake.

(Nagy et al., 2017)

Starvation-induced formation of acidic autolysosomes (visualized by LysoTracker staining) is blocked in Atg16^d67 fat cell somatic clones in mosaic third instar larvae (unlike in the surrounding control cells) as well as in Atg16^d67 homozygous larvae.

Atg16^d67 mutant adult flies show accumulations of protein aggregates in the brain neurons and display reduced climbing ability in the negative geotaxis assay and shorter lifespan compared to controls.

(Varga et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg16^d67 has abnormal cell number | adult stage phenotype, suppressible | partially by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 has abnormal cell number | adult stage phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 has abnormal immune response | adult stage phenotype, suppressible by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 has abnormal immune response | adult stage phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg16^d67 has adult posterior midgut epithelium phenotype, suppressible | partially by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 has enteroendocrine cell | adult stage phenotype, suppressible by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 has enteroblast | adult stage phenotype, suppressible | partially by sli^EY10695/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 has adult posterior midgut epithelium phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 has enteroendocrine cell | adult stage phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 has enteroblast | adult stage phenotype, suppressible by robo2^EP2582/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Both the increased proportion of pre-enteroendocrine cells and the reciprocal decreased proportion of mature enteroendocrine cells observed in the adult gut of Atg16^d67 homozygotes are fully suppressed by the expression of robo2^EP2582 under the control of Scer\GAL4^esg.PU, and are fully and partially suppressed, respectively, by the expression of sli^EY10695 under the control of Scer\GAL4^esg.PU; their severely decreased lifespan upon oral infection with Pseudomonas aeruginosa or Enterococcus faecalis is also suppressed by the expression of sli^EY10695 or robo2^EP2582 under the control of Scer\GAL4^esg.PU.

(Nagy et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Atg16^d67 is rescued by Atg16^{UAS.c.Tag:FLAG}/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 is rescued by Atg16^ΔAutD.UAS/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 is rescued by Atg16^{ΔAutD+Linker.UAS}/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Atg16^d67 is rescued by Atg16^Tag:HA

(Varga et al., 2016)

Not rescued by

Atg16^d67 is not rescued by Atg16^ΔWD40.UAS/Scer\GAL4^esg.PU

(Nagy et al., 2017)

Comments

Both the increased proportion of pre-enteroendocrine cells and the reciprocal decreased proportion of mature enteroendocrine cells observed in the adult gut of Atg16^d67 homozygotes are fully suppressed by the expression of Atg16^{Scer\UAS.c.T:Zzzz\FLAG}, Atg16^{ΔAutD.Scer\UAS} or Atg16^{ΔAutD+Linker.Scer\UAS}, but not Atg16^{ΔWD40.Scer\UAS}, under the control of Scer\GAL4^esg.PU.

The severely decreased lifespan of Atg16^d67 homozygotes upon oral infection with Pseudomonas aeruginosa or Enterococcus faecalis is suppressed by the expression of Atg16^{ΔAutD+Linker.Scer\UAS}, but not Atg16^{ΔWD40.Scer\UAS}, under the control of Scer\GAL4^esg.PU.

(Nagy et al., 2017)

The abrogated formation of acidic lysosomes in fat body cells in Atg16^d67 homozygous third instar larvae as well as the accumulation of protein aggregates in brain neurons in adult flies, their reduced climbing ability and shorter lifespan can be rescued by combination with Atg16^T:Ivir\HA1.

(Varga et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atg16^d67

(Bhattacharjee et al., 2022, Juhasz, 2017.6.13, Nagy et al., 2017, Varga et al., 2016)

Atg16^Δ67

(Vincze et al., 2026, Szabó et al., 2023, Jipa et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (7)

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