Cells were treated with the robo ligand sli for 10 minutes before the pull down assay.
No interaction of robo with Pak is detected in yeast two hybrid assays. The robo-Pak interaction in transfected cells is likely mediated by dock, which can bind robo and Pak directly.
Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.
