FlyBase Gene Report: Dmel\Ork1

General Information

Symbol

Dmel\Ork1

Species

D. melanogaster

Name

Open rectifier K⁺ channel 1

Annotation Symbol

CG1615

Feature Type

protein_coding_gene

FlyBase ID

FBgn0017561

Gene Model Status

Current

Stock Availability

21 publicly available

Gene Summary

Open rectifier K[+] channel 1 (Ork1) encodes a member of the two pore open rectifier potassium channel gene family. It contributes to the regulation of membrane resting potential. The primary gating mechanism for the product of Ork1 appears to be based on phosphorylation of multiple serine, threonine and tyrosine residues in the large intracellular C-terminal tail. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dORK, ork, Open rectifier K+ channel 1, dORK1, KCNK0

Key Links

Genomic Location

Cytogenetic map

9F7-9F8

Sequence location

X:10,878,054..10,891,250 [-]

Recombination map

1-33

RefSeq locus

NC_004354 REGION:10878054..10891250

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables potassium ion leak channel activity

inferred from direct assay

(Kollewe et al., 2009)

enables protein homodimerization activity

inferred from physical interaction with FLYBASE:Ork1; FB:FBgn0017561

(Kollewe et al., 2009)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables outward rectifier potassium channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000108561

(GO Reference Genome Project, 2011-)

enables potassium channel activity

inferred from electronic annotation with InterPro:IPR003280

(InterPro Project Members, 2004-)

enables potassium ion leak channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000108561

(GO Reference Genome Project, 2011-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in locomotor rhythm

inferred from mutant phenotype

(Nitabach et al., 2006)

involved_in potassium ion transmembrane transport

inferred from direct assay

(Kollewe et al., 2009)

involved_in regulation of heart rate

inferred from mutant phenotype

(Lalevee et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in potassium ion transmembrane transport

inferred from electronic annotation with InterPro:IPR003280

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000108561

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from direct assay

(Kollewe et al., 2009)

part_of potassium channel complex

inferred from direct assay

(Kollewe et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR003280

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000108561

(GO Reference Genome Project, 2011-)

located_in plasma membrane

inferred from sequence or structural similarity with SGD:S000003629

(Goldstein et al., 1996)

inferred from sequence or structural similarity with UniProtKB:O00180

(Goldstein et al., 1996)

Gene Group (FlyBase)

TWO-PORE DOMAIN K⁺ CHANNEL SUBUNITS

Protein Family (UniProt)

Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. (Q94526)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

TWO-PORE DOMAIN K⁺ CHANNEL SUBUNITS -: The two-pore domain K⁺ (K2P) channels possess four transmembrane domains and two pore domains; they assemble as dimers. K2P channels have been implicated in a number of processes such as maintaining resting potential (leak K⁺ conductance), responding to volatile anesthetics, chemo- and mechanosensation. (Adapted from FBrf0188365 and PMID:11121510).

Protein Function (UniProtKB)

Background potassium channel. Rectification is dependent on external potassium concentration. Acts as an outwardly rectifying channel but as external potassium levels increase, this is reversed.

(UniProt, Q94526)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ork1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q94526)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.52

Supported by strand-specific RNA-Seq data.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ork1-RA

FBtr0073405

NM_167259

4100

1001

Ork1-RB

FBtr0073404

NM_078557

3979

1001

Ork1-RC

FBtr0343584

NM_001298168

4033

1019

Ork1-RD

FBtr0346304

NM_001298167

4409

1001

Additional Transcript Data and Comments

Reported size (kB)

2.8 (northern blot)

(Goldstein et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ork1-PA

FBpp0073261

109.3

1001

9.96

FBpp0073260

109.3

1001

9.96

FBpp0310181

111.5

1019

10.24

FBpp0312045

109.3

1001

9.96

Q94526

NP_001285096

AHN59566

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1001 aa isoforms: Ork1-PA, Ork1-PB, Ork1-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

618 (aa); 68 (kD predicted)

(Goldstein et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ork1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.71

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

larval stage

embryonic/larval heart

(Lalevee et al., 2006)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & larval stage & adult stage

(Goldstein et al., 1996)

adult stage

adult brain

(Goldstein et al., 1996)

adult muscle system

(Goldstein et al., 1996)

adult stage & oogenesis

ovary

(Goldstein et al., 1996)

Additional Descriptive Data

Ork1 transcript is expressed in the spontaneously contracting myocytes of the larval heart.

(Lalevee et al., 2006)

A 2.8kb Ork1 transcript is present in adults and is also detected at low levels in embryos and larvae. In situ hybridization reveals that Ork1 transcripts are widespread in adult flies and are particularly abundant in muscle, brain and ovary.

(Goldstein et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in membrane

inferred from direct assay

(Kollewe et al., 2009)

part_of potassium channel complex

inferred from direct assay

(Kollewe et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ork1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ork1

View exonic expression by tissue for Dmel\Ork1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0017561

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32020/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0017561

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0017561

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0017561

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1615

Flygut - An atlas of the Drosophila adult midgut

FBgn0017561

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ork1

Transgenic constructs containing regulatory region of Ork1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

lethal, with Scer\GAL4^elav-C155

Ork1^{Δ-C.UAS.EGFP}

viable

Ork1^d09258

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ork1^GD3306

viable, with Scer\GAL4^elav-C155

Ork1^{Δ-NC.UAS.EGFP}

viable, with Scer\GAL4^pnr-MD237

Ork1^GD3306

viable, with Scer\GAL4^tin.CΔ4

Ork1^GD3306

Sterility

Allele

fertile

Ork1^d09258

Other Phenotypes

Allele

abnormal behavior | larval stage, with Scer\GAL4^ppk.1.9

Ork1^{Δ-C.UAS.EGFP}

abnormal chemosensory behavior | adult stage, with Scer\GAL4^Dsk.PP

Ork1^{Δ-C.UAS.EGFP}

abnormal chemosensory behavior | adult stage, with Scer\GAL4^Ilp2.215-3

Ork1^{Δ-C.UAS.EGFP}

abnormal developmental rate, with Scer\GAL4^ppk.1.9

Ork1^{Δ-C.UAS.EGFP}

abnormal eclosion rhythm, with Scer\GAL4^Ptth.cMa

Ork1^{Δ-C.UAS.EGFP}

abnormal feeding behavior | adult stage, with Scer\GAL4^Dsk.PP

Ork1^{Δ-C.UAS.EGFP}

abnormal feeding behavior | adult stage, with Scer\GAL4^Gr64f.9.7

Ork1^{Δ-C.UAS.EGFP}

abnormal feeding behavior | adult stage, with Scer\GAL4^Ilp2.215-3

Ork1^{Δ-C.UAS.EGFP}

abnormal feeding behavior | adult stage, with Scer\GAL4^NP0115

Ork1^{Δ-C.UAS.EGFP}

abnormal feeding behavior | adult stage, with Scer\GAL4^NP0883

Ork1^{Δ-C.UAS.EGFP}

abnormal feeding behavior | adult stage, with Scer\GAL4^NP1076

Ork1^{Δ-C.UAS.EGFP}

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

Ork1^{Δ-C.UAS.EGFP}

abnormal neuroanatomy, with Scer\GAL4^Mz19

Ork1^{Δ-C.UAS.EGFP}

abnormal neuroanatomy | first instar larval stage, with Scer\GAL4^Lim3b

Ork1^UAS.cUa

abnormal neuroanatomy | somatic clone, with Scer\GAL4^ppk.PU

Ork1^{Δ-C.UAS.EGFP}

abnormal neurophysiology, with Scer\GAL4^Mz19

Ork1^{Δ-C.UAS.EGFP}

abnormal phototaxis | third instar larval stage, with Scer\GAL4^NP394

Ork1^{Δ-C.UAS.EGFP}

abnormal phototaxis | third instar larval stage, with Scer\GAL4^NP423

Ork1^{Δ-C.UAS.EGFP}

abnormal phototaxis | third instar larval stage, with Scer\GAL4^NP867

Ork1^{Δ-C.UAS.EGFP}

abnormal sleep, with Scer\GAL4^104y

Ork1^{Δ-C.UAS.EGFP}

abnormal visual behavior, with Scer\GAL4^tim.UAS

Ork1^{Δ-NC.UAS.EGFP}

decreased body size, with Scer\GAL4^ppk.1.9

Ork1^{Δ-C.UAS.EGFP}

Phenotype manifest in

Allele

abdominal anterior ventral multidendritic neuron vdaa | somatic clone, with Scer\GAL4^ppk.PU

Ork1^{Δ-C.UAS.EGFP}

embryonic/larval heart

embryonic/larval heart, with Scer\GAL4^NP1029

embryonic/larval olfactory receptor neuron | first instar larval stage, with Scer\GAL4^Lim3b

Ork1^UAS.cUa

Kenyon cell, with Scer\GAL4^Mz19

Ork1^{Δ-C.UAS.EGFP}

presynaptic active zone, with Scer\GAL4^Mz19

Ork1^{Δ-C.UAS.EGFP}

projection neuron, with Scer\GAL4^Mz19

Ork1^{Δ-C.UAS.EGFP}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (51)

Hsap\KCNK10

10 of 14

Yes

Hsap\KCNK2

9 of 14

Yes

Hsap\KCNK4

8 of 14

Yes

Hsap\KCNK5

8 of 14

Yes

Hsap\KCNK16

8 of 14

Yes

Hsap\KCNK17

8 of 14

Yes

Hsap\KCNK6

6 of 14

Yes

Hsap\KCNK18

6 of 14

Hsap\KCNK1

5 of 14

Hsap\KCNK7

5 of 14

Yes

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (43)

Rnor\Kcnk10

9 of 14

Yes

Rnor\Kcnk2

8 of 14

Yes

Rnor\Kcnk4

8 of 14

Yes

Rnor\Kcnk5

7 of 14

Yes

Rnor\Kcnk16

7 of 14

Yes

Rnor\Kcnk6

6 of 14

Yes

Rnor\Kcnk18

6 of 14

Rnor\Kcnk1

5 of 14

Yes

Rnor\Kcnk7

5 of 14

Yes

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Ptprq

1 of 14

Mus musculus (laboratory mouse) (45)

Mmus\Kcnk10

9 of 14

Yes

Mmus\Kcnk2

8 of 14

Yes

Mmus\Kcnk4

8 of 14

Yes

Mmus\Kcnk5

7 of 14

Yes

Mmus\Kcnk16

7 of 14

Yes

Mmus\Catsperz

6 of 14

Yes

Mmus\Kcnk1

6 of 14

Yes

Mmus\Kcnk6

5 of 14

Mmus\Kcnk7

5 of 14

Yes

5 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (51)

Xtro\kcnk10

6 of 13

Yes

Xtro\kcnk16

6 of 13

Yes

Xtro\XB5727110

6 of 13

Yes

Xtro\kcnk2

4 of 13

Yes

Xtro\kcnk4

4 of 13

Yes

Xtro\LOC100487239

4 of 13

Yes

Xtro\kcnk1

3 of 13

Yes

Xtro\kcnk5

3 of 13

Yes

Xtro\kcnk7

3 of 13

Yes

Xtro\kcnk9

3 of 13

Xtro\kcnk18

3 of 13

Xtro\LOC101731658

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Xtro\kcnk15

2 of 13

Xtro\LOC100488610

2 of 13

Yes

Xtro\ankrd46

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\kcnk13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\msx1

1 of 13

Xtro\rora

1 of 13

Xtro\tmem184c

1 of 13

Danio rerio (Zebrafish) (57)

Drer\kcnk10a

8 of 14

Yes

Drer\kcnk10b

8 of 14

Yes

Drer\kcnk5a

7 of 14

Yes

Drer\kcnk5b

7 of 14

Yes

Drer\kcnk2a

6 of 14

Yes

Drer\kcnk17

6 of 14

Yes

Drer\kcnk18

6 of 14

Drer\kcnk1b

5 of 14

Yes

Drer\kcnk2b

5 of 14

Yes

Drer\kcnk4a

5 of 14

Yes

Drer\kcnk6

5 of 14

Yes

Drer\kcnk1a

4 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

Yes

Drer\kcnk15

3 of 14

Drer\si:ch211-261a10.5

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch73-334d15.4

1 of 14

Drer\si:dkey-43k4.5

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (62)

Cele\twk-7

6 of 14

Yes

Cele\twk-28

6 of 14

Yes

Cele\twk-1

5 of 14

Yes

Cele\twk-13

5 of 14

Cele\twk-16

5 of 14

Yes

5 of 14

5 of 14

5 of 14

4 of 14

Yes

Cele\sup-9

4 of 14

Cele\twk-2

4 of 14

Cele\twk-3

4 of 14

Yes

Cele\twk-4

4 of 14

Cele\twk-6

4 of 14

Yes

Cele\twk-9

4 of 14

Yes

Cele\twk-12

4 of 14

Yes

Cele\twk-23

4 of 14

Cele\twk-26

4 of 14

Cele\twk-29

4 of 14

Yes

Cele\twk-33

4 of 14

Yes

Cele\twk-34

4 of 14

Yes

Cele\twk-35

4 of 14

Yes

Cele\twk-36

4 of 14

Yes

Cele\twk-45

4 of 14

Cele\twk-46

4 of 14

Yes

Cele\twk-47

4 of 14

Yes

Cele\twk-5

3 of 14

Yes

Cele\twk-10

3 of 14

Yes

Cele\twk-17

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Cele\twk-43

3 of 14

Yes

Cele\twk-44

3 of 14

Yes

Cele\unc-58

3 of 14

Yes

Cele\C27F2.6

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Cele\twk-22

2 of 14

Cele\twk-25

2 of 14

Yes

Cele\twk-31

2 of 14

Cele\twk-49

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (20)

Agam\AgaP_AGAP002224

12 of 12

Yes

5 of 12

4 of 12

4 of 12

4 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\KCO1

5 of 13

Yes

Atha\KCO2

5 of 13

Yes

Atha\KCO3

5 of 13

Yes

Atha\KCO5

5 of 13

Yes

Atha\KCO6

5 of 13

Yes

Atha\TPK4

5 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\TOK1

7 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (1)

Ecol\kch

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ork1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (19)

8 of 13

7 of 13

7 of 13

7 of 13

6 of 13

6 of 13

6 of 13

5 of 13

5 of 13

5 of 13

2 of 13

1 of 13

Lar

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

cardiac arrhythmia (postulated), KCNK-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 4 )

Allele

Disease

Evidence

References

Ork1^d01340

model of heart conduction disease

CEA

(Lalevee et al., 2006)

Ork1^e02481

model of heart conduction disease

CEA

(Lalevee et al., 2006)

Ork1^RNAi.UAS

model of heart conduction disease

CEA

(Lalevee et al., 2006)

Ork1^UAS.FL.cLa

model of heart conduction disease

CEA

(Lalevee et al., 2006)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KCNK10; potassium two pore domain channel subfamily K member 10

10 of 14

605873

KCNK2; potassium two pore domain channel subfamily K member 2

9 of 14

603219

KCNK4; potassium two pore domain channel subfamily K member 4

8 of 14

POTASSIUM CHANNEL, SUBFAMILY K, MEMBER 4; KCNK4

FACIAL DYSMORPHISM, HYPERTRICHOSIS, EPILEPSY, INTELLECTUAL/DEVELOPMENTAL DELAY, AND GINGIVAL OVERGROWTH SYNDROME; FHEIG

KCNK5; potassium two pore domain channel subfamily K member 5

8 of 14

603493

KCNK16; potassium two pore domain channel subfamily K member 16

8 of 14

607369

KCNK17; potassium two pore domain channel subfamily K member 17

8 of 14

607370

KCNK6; potassium two pore domain channel subfamily K member 6

6 of 14

603939

KCNK18; potassium two pore domain channel subfamily K member 18

6 of 14

613655

MIGRAINE WITH AURA, SUSCEPTIBILITY TO, 13; MGR13

KCNK1; potassium two pore domain channel subfamily K member 1

5 of 14

601745

KCNK7; potassium two pore domain channel subfamily K member 7

5 of 14

603940

KCNK9; potassium two pore domain channel subfamily K member 9

4 of 14

605874

BIRK-BAREL SYNDROME; BIBARS

Birk-Barel syndrome

KCNK3; potassium two pore domain channel subfamily K member 3

3 of 14

603220

primary pulmonary hypertension

KCNK15; potassium two pore domain channel subfamily K member 15

3 of 14

607368

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ork1 - osk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Hurd et al., 2016)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

Ork1

(Hu et al., 2015)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58437

DroID - A comprehensive database of gene and protein interactions.

FBgn0017561

MIST (protein-protein) - An integrated Molecular Interaction Database

32020

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-33

Cytogenetic map

9F7-9F8

Sequence location

X:10,878,054..10,891,250 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

9F7-9F8

Limits computationally determined from genome sequence between P{EP}sesB^EP319&P{EP}Imp^EP760 and P{EP}EP1321^EP1321&P{EP}CG2061^EP1537

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

10A1-10A2

(Goldstein, 1996.4.17)

10A1-10A2

(determined by in situ hybridisation)

(Goldstein et al., 1996)

Experimentally Determined Recombination Data

Location

1-33

(FlyBase, 2016)

1-33.8

(Comeron et al., 2012)

(Goldstein, 1996.4.17)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (21)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

81072

w^* P{R14E01-FLPD5}attP18

19309

w¹¹¹⁸ P{XP}Ork1^d09258

85992

w¹¹¹⁸ P{XP}Ork1^d01340

6586

y¹ w^*; P{UAS-Ork1.Δ-C}2

6587

y¹ w^*; P{UAS-Ork1.Δ-NC}1

86021

w¹¹¹⁸ PBac{RB}Ork1^e02481

Kyoto

108839

y¹ w^*; P{UAS-Ork1.Δ-C}2

108840

y¹ w^*; P{UAS-Ork1.Δ-NC}1

1615R-1

1615R-2

v104883

v40953

w¹¹¹⁸; P{GD3306}v40953

More stocks available...

Genomic Clones (6)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (22)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI03618

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0017561

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0017561

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Ork1 encodes a K⁺-selective leak channel with two pore domains.

(Goldstein et al., 1996)

S.cerevisiae Scer\trk1 Scer\trk2 mutants defective in K⁺ uptake are functionally complemented by Ork1.

(Goldstein et al., 1996)

Relationship to Other Genes

Source for database merge of

Source for merge of: Ork1 anon-WO0161006.1 anon-WO0161006.22 anon-WO0161006.23

(FlyBase, 1996-)

Additional comments

Source for merge of Ork1 anon-WO0161006.1 anon-WO0161006.22 anon-WO0161006.23 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Ork1 CG1615

(Wang, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

CG1615

(Walcott et al., 2018, Hurd et al., 2016, Tsubouchi et al., 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Döring et al., 2006, Lalevee et al., 2006)

DorK

(Prieto-Godino et al., 2012)

K_2P

(Kollewe et al., 2009)

KCNK0

(Ben-Abu et al., 2009, Zilberberg et al., 2001, Goldstein, 1996.4.17)

ORK

(Hodge et al., 2006, Srahna et al., 2006)

ORK-1

(Buckingham et al., 2005)

ORK1

(Hadjisavva and Cooper, 2025, Taul et al., 2025, Elliott and Cooper, 2024, Neckameyer and Argue, 2013, Rodriguez Moncalvo and Campos, 2009, Döring et al., 2006, Ilan et al., 1999, Goldstein et al., 1997, Goldstein, 1996.4.17, Goldstein et al., 1996)

Ork

(Pauls et al., 2021, Nagy et al., 2019)

anon-WO0161006.1

anon-WO0161006.22

anon-WO0161006.23

d-ORK1

(Ilan and Goldstein, 1998, Zilberberg and Goldstein, 1998)

dORK

(Fogle et al., 2015, Sun et al., 2014, Gong et al., 2010, Liu et al., 2010, Sheeba et al., 2010, Parisky et al., 2008, Sheeba et al., 2008, Sheeba et al., 2008, Wu et al., 2008, Park and Griffith, 2006, Mazzoni et al., 2005, Nitabach et al., 2005, Lu et al., 2003, Nitabach et al., 2002)

dORK1

(Gupta et al., 2016, Liu et al., 2012, Kremer et al., 2010)

dORKC1

(Lone et al., 2015)

dOrk

(Yang et al., 2014)

dOrk1

(Luo et al., 2013, Wegman et al., 2010)

ork

(Lowe et al., 2024, Mandel et al., 2018, Dahal et al., 2017)

ork1

(Schotthöfer and Bohrmann, 2020, Zhang et al., 2017, Sénatore et al., 2010)

Name Synonyms

Open rectifier K+ channel 1

(Shafer, 2025, Tsubouchi et al., 2012, Guan et al., 2011)

Open rectifier K+ channel 1

(Zhao et al., 2008, Monier et al., 2007, Goldstein et al., 1996)

open rectifier potassium channel

(Wu et al., 2008)

Secondary FlyBase IDs

FBgn0046859
FBgn0046860
FBgn0046861

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32020

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q94526

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q94526

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q94526

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0017561

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32020/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0017561

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0017561

FlyMine - An integrated database for Drosophila genomics

FBgn0017561

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1615

MARRVEL_MODEL - MARRVEL (model organism gene)

32020

Linkouts

BioGRID - A database of protein and genetic interactions.

58437

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0017561

DroID - A comprehensive database of gene and protein interactions.

FBgn0017561

DRSC - Results frm RNAi screens

FBgn0017561

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0017561

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0017561

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1615

Flygut - An atlas of the Drosophila adult midgut

FBgn0017561

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0017561

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008009

MIST (protein-protein) - An integrated Molecular Interaction Database

32020

References (141)

The following external sites may use different assemblies or annotations than FlyBase.

-0.71

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