FlyBase Allele Report: Scer\GAL4[NP0883]

General Information

Symbol

Scer\GAL4^NP0883

Species

S. cerevisiae

Name

FlyBase ID

FBal0316877

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}NP0883

Carried in Construct

Also Known As

NP883-GAL4

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}NP0883

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:8,566,567..8,567,263

(Landayan et al., 2021, Sterne et al., 2021, McKellar et al., 2020, Olson et al., 2019, Zhan et al., 2016, Pool et al., 2014, Sun et al., 2014, Flood et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

CNS surface glial cell

(Olson et al., 2019)

antennal disc

(Olson et al., 2019)

wing pouch

(Olson et al., 2019)

adult stage

adult feeding interneuron

(Sterne et al., 2021, Flood et al., 2013)

subesophageal zone neuron | subset

(Flood et al., 2013)

adult antennal lobe projection neuron | subset

(Flood et al., 2013)

adult central nervous system | restricted

(Flood et al., 2013)

Additional Information

Statement

Reference

ScerGAL4^NP0883 drives expression in around 70 cells in the adult brain and in around 50 cells in the thoracico-abdominal ganglion. Labelled cells in the brain are mainly located in the adult gnathal ganglion and in the region of the antennal lobe/lateral horn.

(Flood et al., 2013)

Marker for

adult feeding interneuron

(Sterne et al., 2021)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior | adult stage, with Ork1^{Δ-C.UAS.EGFP}

(Sun et al., 2014)

abnormal feeding behavior | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Zhan et al., 2016, Pool et al., 2014, Sun et al., 2014)

increased rate of movement | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Pool et al., 2014)

Phenotype Manifest In

proboscis | heat sensitive, with TrpA1^UAS.(B).cKa

(Pool et al., 2014)

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of Scer\GAL4^NP0883 cells in adults significantly increases food intake of satiated flies, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with orange light) of Scer\GAL4^NP0883 neurons in the absence of food prior to testing in adults does not affect food intake, compared to controls.

(Zhan et al., 2016)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^NP0883 cells in fed adult females significantly increases consumption of water, sugar solution and bitter solution, and significantly increases spontaneous proboscis extensions, compared to controls.

Inactivation (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^NP0883 cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, in fed adult females does not significantly affect consumption of water, sugar solution, or bitter solution, compared to controls.

(Pool et al., 2014)

Inhibition (using Ork1^{Δ-C.UAS.EGFP}) of Scer\GAL4^NP0883 cells in adults given a choice between sucrose solution and water results in significantly reduced sucrose, but not water, consumption, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of Scer\GAL4^NP0883 cells in adults given a choice between sucrose solution and water results in significantly increased water, but not sucrose, consumption, compared to controls; activation in adults given access to only a single solution results in significantly increased sucrose, xylitol, caffeine or water consumption, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa with an infrared heat pulse to the head) of Scer\GAL4^NP0883 cells in adults significantly increases proboscis extensions, compared to controls.

(Sun et al., 2014)

Activation of Scer\GAL4^NP0883-expressing neurons results in continuous feeding of satiated flies. Increased proboscis extension is also seen in the absence of food. Satiated Scer\GAL4^NP5137-expressing flies also show increased pharyngeal pumping (used for swallowing food) compared with satiated wild type flies.

Suppression of activity in Scer\GAL4^NP0883-expressing neurons results in abolishment of natural feeding behavior in response to sucrose.

(Flood et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

103803

w^*; P{GawB}NP0883 / CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^NP0883

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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