FlyBase Allele Report: Dmel\Ork1[Δ-C.UAS.EGFP]

General Information

Symbol

Dmel\Ork1^{Δ-C.UAS.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0135891

Feature type

allele

Associated gene

Dmel\Ork1

Associated Insertion(s)

Carried in Construct

P{UAS-Ork1.Δ-C}

Also Known As

UAS-ORK1ΔC, UAS-dORK, UAS-orkΔC, UAS-dORKΔC, UAS-ork^Δc, UAS-Ork1.Δ-C

Key Links

Transgenic product class

inframe_deletion

(Nitabach et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Nitabach et al., 2002)

Mutagen

in vitro construct

(Nitabach, 2002.5.23, Nitabach et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Ork1.Δ-C}

Cytology

Description

UASt regulatory sequences drive expression of a truncated form of Ork1 (lacking amino acids 299 to 1001) that is tagged between amino acid residues 288 and 289 with EGFP. Encodes a conducting form of Ork1.

(Nitabach et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ork1

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | larval stage, with Scer\GAL4^ppk.1.9

(Wegman et al., 2010)

abnormal chemosensory behavior | adult stage, with Scer\GAL4^Dsk.PP

(Söderberg et al., 2012)

abnormal chemosensory behavior | adult stage, with Scer\GAL4^Ilp2.215-3

(Söderberg et al., 2012)

abnormal developmental rate, with Scer\GAL4^ppk.1.9

(Wegman et al., 2010)

abnormal eclosion rhythm, with Scer\GAL4^Ptth.cMa

(Selcho et al., 2017)

abnormal feeding behavior | adult stage, with Scer\GAL4^Dsk.PP

(Söderberg et al., 2012)

abnormal feeding behavior | adult stage, with Scer\GAL4^Gr64f.9.7

(Sun et al., 2014)

abnormal feeding behavior | adult stage, with Scer\GAL4^Ilp2.215-3

(Söderberg et al., 2012)

abnormal feeding behavior | adult stage, with Scer\GAL4^NP0115

(Sun et al., 2014)

abnormal feeding behavior | adult stage, with Scer\GAL4^NP0883

(Sun et al., 2014)

abnormal feeding behavior | adult stage, with Scer\GAL4^NP1076

(Sun et al., 2014)

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

(Nitabach et al., 2002)

abnormal neuroanatomy, with Scer\GAL4^Mz19

(Kremer et al., 2010)

abnormal neuroanatomy | somatic clone, with Scer\GAL4^ppk.PU

(Yang et al., 2014)

abnormal neurophysiology, with Scer\GAL4^Mz19

(Kremer et al., 2010)

abnormal phototaxis | third instar larval stage, with Scer\GAL4^NP394

(Gong et al., 2010)

abnormal phototaxis | third instar larval stage, with Scer\GAL4^NP423

(Gong et al., 2010)

abnormal phototaxis | third instar larval stage, with Scer\GAL4^NP867

(Gong et al., 2010)

abnormal sleep, with Scer\GAL4^104y

(Liu et al., 2012)

decreased body size, with Scer\GAL4^ppk.1.9

(Wegman et al., 2010)

lethal, with Scer\GAL4^elav-C155

(Nitabach et al., 2002)

Phenotype Manifest In

abdominal anterior ventral multidendritic neuron vdaa | somatic clone, with Scer\GAL4^ppk.PU

(Yang et al., 2014)

embryonic/larval heart, with Scer\GAL4^NP1029

(Lalevee et al., 2006)

Kenyon cell, with Scer\GAL4^Mz19

(Kremer et al., 2010)

presynaptic active zone, with Scer\GAL4^Mz19

(Kremer et al., 2010)

projection neuron, with Scer\GAL4^Mz19

(Kremer et al., 2010)

Detailed Description

Statement

Reference

Hsap\RELA^{AD.R92A10.T:Zzzz\Zip+} Scer\GAL4^{DBD.R17D06.T:Zzzz\Zip-}-mediated expression of Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} does not trigger basal rhythmic depolarization in electroretinograms.

(Hu et al., 2015)

Single v'ada class IV dendritic arborization neuron cells expressing Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ppk.PU show a ventral shift in the position of their presynaptic terminals compared to controls. The topography of the presynaptic terminals of ddaC or vdaB class IV da neurons is unaffected if they express Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ppk.PU as single cell clones.

(Yang et al., 2014)

Flies expressing Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^104Y show reduced night-time sleep compared to controls.

(Liu et al., 2012)

Expression of Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of any one of Scer\GAL4^NP423, Scer\GAL4^NP867 or Scer\GAL4^NP394 leads to positive larval phototaxis, whereas controls show negative phototaxis.

(Gong et al., 2010)

Whole-cell current-clamp recordings from the somata in isolated, intact brain preparations reveals that expression of Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mz19 efficiently silences projection neurons. The silencing of the Scer\GAL4^Mz19-positive projection neurons induces increased active zone density. The microglomeruli formed by the clawed dendritic endings of mushroom body Kenyon cells associated with silenced projection neurons are more numerous compared with controls.

(Kremer et al., 2010)

Electrical silencing of mdIV neurons through expression of Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ppk.1.9 results in larvae that fail to display intermediate surfacing transition (IST) behavior (emerging from the food transiently to to roam the food surface, prior to complete food exit) seen in wild type. Eventual food exit is slightly delayed compared to controls. the resulting adult flies are smaller than their wild type counterparts.

(Wegman et al., 2010)

Expression of Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} in the whole cardiac tube during larval stages, driven by Scer\GAL4^NP1029, results in the arrest of heart beating.

(Lalevee et al., 2006)

Expression of Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ato.3.6 does not affect dorsal cluster neuron axon extension across the optic chiasm in adult flies.

(Srahna et al., 2006)

Most embryos expressing Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^elav-C155 fail to hatch and the rest show very sluggish movement as first instar larvae and fail to reach the second instar. Expression of Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^P2.4.Pdf does not alter the number of adult lateral pacemaker neurons, and they show their normal dorso-medial projection into the central brain. 72% of flies expressing P{UAS-Ork1.Δ-C}1 under the control of Scer\GAL4^P2.4.Pdf are arrhythmic with respect to free-running locomotor activity. In LD conditions, flies expressing Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^P2.4.Pdf begin to increase their activity about 2 hours before lights-off (as do wild-type flies).

(Nitabach et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\RELA^AD.R92A10, Ork1^{Δ-C.UAS.EGFP}, Scer\GAL4^DBD.R17D06 is a suppressor | partially of abnormal neurophysiology phenotype of Ih^f03355

(Hu et al., 2015)

Ork1^{Δ-C.UAS.EGFP}, Scer\GAL4^ppk.1.9 is a suppressor of decreased body size phenotype of Bhal\NaChBac^UAS.EGFP, Scer\GAL4^ppk.1.9

(Wegman et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^c202a, Scer\GAL4^21D, Ork1^{Δ-C.UAS.EGFP} is a non-suppressor of abnormal neurophysiology phenotype of Ih^f03355

(Hu et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Neuronal silencing of mdIV neurons through expression of Ork1^{Δ-C.Scer\UAS.T:Avic\GFP-EGFP} suppresses the reduction in adult body size seen when Bhal\NaChBac^{Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^ppk.1.9.

(Wegman et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

6586

y¹ w^*; P{UAS-Ork1.Δ-C}2

8928

w^*; P{UAS-Ork1.Δ-C}1/TM6C, Sb¹