FlyBase Allele Report: Scer\GAL4[21D]

General Information

Symbol

Scer\GAL4^21D

Species

S. cerevisiae

Name

FlyBase ID

FBal0241837

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GAL4}21D

Carried in Construct

Also Known As

21D-Gal4, L2-GAL4, L2, L2^21Dhh-Gal4

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GAL4}21D

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GAL4}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

lamina monopolar neuron L2

(Bahl et al., 2015, Hasegawa et al., 2013, Takemura et al., 2011, Kolodziejczyk et al., 2008, Rister et al., 2007)

Additional Information

Statement

Reference

Drives expression in lamina monopolar neurons L2.

(FlyBase Curators, 2017)

Marker for

lamina monopolar neuron L2

(Bahl et al., 2015, Hasegawa et al., 2013, Takemura et al., 2011)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | heat sensitive, with shi^1.UAS

(de Vries and Clandinin, 2012)

abnormal optomotor response | heat sensitive, with Scer\GAL4^NP1086, shi^1.UAS

(Rister et al., 2007)

abnormal optomotor response | heat sensitive, with shi^1.UAS

(Rister et al., 2007)

abnormal visual behavior | heat sensitive, with shi^1.UAS

(Katsov and Clandinin, 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Blocking neural transmission in Scer\GAL4^21D (L2)-expressing neurons results in defects in turning responses to rotational visual motion, exhibiting strong deficits in turning to moving dark edges (a transition from brighter to darker), although some dark edge response remains. In an opposing edge assay (in which light and dark edges move in opposite directions) the flies turn in the direction of the light edge motion.

When neurons expressing Scer\GAL4^c202a (L1) and Scer\GAL4^21D (L2) are both silenced the flies do not respond to rotational stimuli.

Flies show no turning at all in response to moving dark edges (a transition from brighter to darker) when both Scer\GAL4^21D (L2) and Scer\GAL4⁰⁵⁹⁵ (L3)-expressing neurons are silenced, and turn more strongly towards light edge motion in an opposing edge assay than when only L2 neurons are silenced. The flies display turning responses to light edges.

Additionally silencing Scer\GAL4⁰⁹⁸⁷ (L4)-expressing neurons does not enhance the defects in rotational visual motion response seen when Scer\GAL4^21D (L2)-expressing neurons are silenced.

Blocking neural transmission in Scer\GAL4^21D (L2)-expressing neurons results in reduced modulation of forward walking speed in response to to translational stimuli compared with controls. Responses are decreased to both front-to-back and back-to-front moving square gratings.

Additionally silencing Scer\GAL4^c202a (L1)-expressing neurons does not enhance the defects in translational motion response seen when Scer\GAL4^21D (L2)-expressing neurons are silenced.

Additionally silencing Scer\GAL4⁰⁹⁸⁷ (L4)-expressing neurons does not enhance the defects in translational motion response seen when Scer\GAL4^21D (L2)-expressing neurons are silenced.

Flies show very little modulation of forward walking speed in response to front-to-back translational motion when both Scer\GAL4^21D (L2) and Scer\GAL4⁰⁵⁹⁵ (L3)-expressing neurons are silenced and no detectable slowing in response to back-to-front motion.

(Silies et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hsap\RELA^AD.R92A10, Scer\GAL4^c202a, Scer\GAL4^21D, Scer\GAL4^DBD.R17D06 is an enhancer of abnormal neurophysiology phenotype of Hsap\RELA^AD.R92A10, Ih^HMC03319, Scer\GAL4^DBD.R17D06