FlyBase Allele Report: Dmel\Ih[f03355]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\Ih^f03355

General Information

Symbol

Dmel\Ih^f03355

Species

D. melanogaster

Name

FlyBase ID

FBal0183142

Feature type

allele

Associated gene

Associated Insertion(s)

PBac{WH}Ih^f03355

Carried in Construct

Also Known As

I_h^f03355

Key Links

Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Hu et al., 2015)

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{WH}Ih^f03355

Cytology

Description

Insertion into the exon common to all transcriptional variants of the Ih gene.

(Hu et al., 2015)

Allele components

Component

Use(s)

Inserted element

misexpression element

Regulatory region(s)

binary expression system - regulatory region

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:14,292,113..14,292,113 [-]

(Lee et al., 2025, Lee et al., 2024, Fernandez-Chiappe et al., 2021, Chen and Wang, 2012, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Ih^f03355 homozygotes, Ih^f03355/Ih^f01485 transheterozygotes and Ih^f03355/Df(2R)BSC700 or Ih^f03355/Df(2R)Exel7131 animals exhibit normal light responses but distinctive electroretinogram baseline oscillations. Ih^f03355 mutant photoreceptors, but not wild-type photoreceptors, showed rhythmic depolarization without light stimulation. Rhabdomeral structures are normal in Ih^f03355 mutants.

EM images show no obvious morphological differences between Ih^f03355 and wild-type photoreceptor cartridges.

EM images reveal normal rhabdomere structure in 14-day-old Ih^f03355 mutant flies raised under regular light cycles (12 h light/12 h dark) or in constant darkness.

10 Lux light stimulation evokes a a significantly reduced depolarization in Ih^f03355 mutant photoreceptors, compared to wild type.

With low or high-intensity moving light patterns, Ih^f03355 flies exhibit a reduced ability to track moving patterns.

(Hu et al., 2015)

At old age, mutant flies are very uncoordinated and often fall down with their bodies quivering frequently.

Homozygous and Ih^f03355/Df(2R)Exel7131 flies show an enhanced proboscis extension response (PER) to sugar stimuli compared to controls. Homozygous flies show a higher PER than control flies to caffeine and to berberine.

Under light-dark conditions, mutant flies of both sexes show a reduction in daily sleep time compared to controls. Under constant darkness conditions, the number of sleep bouts of mutant females is increased compared to controls.

(Chen and Wang, 2012)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ih^f03355 has abnormal neurophysiology phenotype, suppressible | partially by Hsap\RELA^AD.R92A10/Ork1^{Δ-C.UAS.EGFP}/Scer\GAL4^DBD.R17D06

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, suppressible | partially by Hsap\RELA^AD.R92A10/VGlut1^JF02689/Scer\GAL4^DBD.R17D06

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, suppressible by cac^H18

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, suppressible | partially by cac^JF02572/Scer\GAL4^elav.PU

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, suppressible | partially by cac^JF02572/Hsap\RELA^AD.R92A10/Scer\GAL4^DBD.R17D06

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, suppressible | partially by Scer\GAL4^ninaE.PU/Ekar^JF03126

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, suppressible | partially by Scer\GAL4^ninaE.PU/Ekar^GL01280

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, suppressible | partially by Ekar^MB00001

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, suppressible by Ctet\tetX^TNT-LC.UAS/Hsap\RELA^AD.R92A10/Scer\GAL4^DBD.R17D06

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, suppressible | partially by Hsap\RELA^AD.R92A10/Bhal\NaChBac^UAS.cNa/Scer\GAL4^DBD.R17D06

(Hu et al., 2015)

NOT suppressed by

Statement

Reference

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by norpA^P24

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^c202a/Scer\GAL4^21D/Ork1^{Δ-C.UAS.EGFP}

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/Nmdar1^JF01961

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/GluClα^KK109167

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/GluRIIA^JF02647

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/GluRIIB^JF03145

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/GluRIIC^JF01854

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/GluRIID^JF02035

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/GluRIIE^JF01962

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/clumsy^KK104645

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/clumsy^GD413

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/KaiR1D^JF01873

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by VGlut1^JF02689/Scer\GAL4^c202a/Scer\GAL4^21D

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/Grik^JF01840

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/ukar^JF03425

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Ctet\tetX^TNT-LC.UAS/Scer\GAL4^c202a/Scer\GAL4^21D

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^c202a/Scer\GAL4^21D/Bhal\NaChBac^UAS.cNa

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Ca-α1T^del

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^elav.PU/Ca-α1D^HMS00294

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/GluRIA^JF02671

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/GluRIB^GD3584

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/GluRIB^KK104241

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/Nmdar2^GD3192

(Hu et al., 2015)

Ih^f03355 has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^ninaE.PU/Nmdar2^GD1621

(Hu et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ih^f03355 is rescued by Scer\GAL4^elav.PU/Ih^UAS.K

(Hu et al., 2015)

Ih^f03355 is rescued by Ih^UAS.K/Hsap\RELA^AD.R92A10/Scer\GAL4^c202a/Scer\GAL4^21D/Scer\GAL4^DBD.R17D06

(Hu et al., 2015)

Partially rescued by

Ih^f03355 is partially rescued by Ih^UAS.K/Hsap\RELA^AD.R92A10/Scer\GAL4^DBD.R17D06

(Hu et al., 2015)

Not rescued by

Ih^f03355 is not rescued by Ih^UAS.K/Scer\GAL4^repo.PU

(Hu et al., 2015)

Ih^f03355 is not rescued by Scer\GAL4^ninaE.PU/Ih^UAS.K

(Hu et al., 2015)

Ih^f03355 is not rescued by Ih^UAS.K/Scer\GAL4^c202a/Scer\GAL4^21D

(Hu et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

85660

w¹¹¹⁸; PBac{WH}Ih^f03355

Notes on Origin

Discoverer

Comments

Comments

Excision of the insertion reverts the phenotype of enhanced proboscis extension response to sugar stimuli.

(Chen and Wang, 2012)

Precise excision of the PBac{WH} element completely reverts the abnormal baseline ERG phenotype.

(Hu et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

I_h^f03355

(Hu et al., 2015, Chen and Wang, 2012)

Ih^f03355

(Lee et al., 2025, Kim et al., 2024, Lee et al., 2024, Trombley et al., 2023, Qiao et al., 2022, Fernandez-Chiappe et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (9)

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