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Reference
Citation
Zhang, Y., Wang, X., Matakatsu, H., Fehon, R., Blair, S.S. (2016). The novel SH3 domain protein Dlish/CG10933 mediates fat signaling in Drosophila by binding and regulating Dachs.  eLife 5(): e16624.
FlyBase ID
FBrf0233684
Publication Type
Research paper
Abstract

Much of the Hippo and planar cell polarity (PCP) signaling mediated by the Drosophila protocadherin Fat depends on its ability to change the subcellular localization, levels and activity of the unconventional myosin Dachs. To better understand this process, we have performed a structure-function analysis of Dachs, and used this to identify a novel and important mediator of Fat and Dachs activities, a Dachs-binding SH3 protein we have named Dlish. We found that Dlish is regulated by Fat and Dachs, that Dlish also binds Fat and the Dachs regulator Approximated, and that Dlish is required for Dachs localization, levels and activity in both wild type and fat mutant tissue. Our evidence supports dual roles for Dlish. Dlish tethers Dachs to the subapical cell cortex, an effect partly mediated by the palmitoyltransferase Approximated under the control of Fat. Conversely, Dlish promotes the Fat-mediated degradation of Dachs.

PubMed ID
PubMed Central ID
PMC5047748 (PMC) (EuropePMC)
Related Publication(s)
Erratum

Correction: The novel SH3 domain protein Dlish/CG10933 mediates fat signaling in Drosophila by binding and regulating Dachs.
Zhang et al., 2016, eLife 5: e22672 [FBrf0233976]

Personal communication to FlyBase

S2R+ and S2-DGRC cell lines used.
Cherbas et al., 2017.4.19, S2R+ and S2-DGRC cell lines used. [FBrf0235313]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Aberrations (1)
    Alleles (33)
    Gene Groups (2)
    Genes (21)
    Physical Interactions (16)
    Cell Lines (2)
    Insertions (4)
    Experimental Tools (5)
    Transgenic Constructs (25)