FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Dlish
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General Information
Symbol
Dmel\Dlish
Species
D. melanogaster
Name
Dachs ligand with SH3s
Annotation Symbol
CG10933
Feature Type
protein_coding_gene
FlyBase ID
FBgn0034264
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Summary
Dachs ligand with SH3s (Dlish) encodes an adapter protein that can bind to the products of dachs, ds, and ft. It is required for the normal growth of wings and legs via Dachsous-Fat signaling. [Date last reviewed: 2018-10-18] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Vam

Key Links
Genomic Location
Cytogenetic map
54E2-54E2
Sequence location
Recombination map
2-84
RefSeq locus
NT_033778 REGION:17723309..17725056
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables cadherin binding
inferred from physical interaction with FLYBASE:ft; FB:FBgn0001075
(Misra and Irvine, 2016)
inferred from physical interaction with FLYBASE:ds; FB:FBgn0284247
(Misra and Irvine, 2016)
enables protein binding
inferred from physical interaction with UniProtKB:M9MRE4
(Zhang et al., 2016)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in establishment of epithelial cell planar polarity
inferred from mutant phenotype
(Misra and Irvine, 2016)
involved_in establishment of planar polarity
inferred from mutant phenotype
(Misra and Irvine, 2016)
involved_in negative regulation of hippo signaling
inferred from mutant phenotype
(Wang et al., 2019, Misra and Irvine, 2016, Zhang et al., 2016)
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process
inferred from genetic interaction with FLYBASE:slmb; FB:FBgn0283468
(Wang et al., 2019)
involved_in protein localization involved in establishment of planar polarity
inferred from mutant phenotype
(Misra and Irvine, 2016)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in protein localization involved in establishment of planar polarity
inferred from biological aspect of ancestor with PANTHER:PTN002689333
(GO Reference Genome Project, 2011-)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in apical cortex
inferred from direct assay
(Zhang et al., 2016)
located_in apicolateral plasma membrane
inferred from direct assay
(Misra and Irvine, 2016)
located_in cytoplasm
inferred from direct assay
(Zhang et al., 2016)
located_in subapical part of cell
inferred from direct assay
(Wang et al., 2019)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in cilium
inferred from biological aspect of ancestor with PANTHER:PTN002689333
(GO Reference Genome Project, 2011-)
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN002689333
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Dachs ligand with SH3s (Dlish) encodes an adapter protein that can bind to the products of dachs, ds, and ft. It is required for the normal growth of wings and legs via Dachsous-Fat signaling. [Date last reviewed: 2018-10-18]
Pathway (FlyBase)
NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
Protein Function (UniProtKB)
Required for the apical cell cortex localization, total cellular level and full activity of dachs.
(UniProt, A1ZAY1)
Summary (Interactive Fly)

planar cell polarity - a Dachs-binding SH3 protein - tethers Dachs to the subapical cell cortex - promotes the Fat-mediated degradation of Dachs - associates with the Dachsous intracellular domain and with the Fat intracellular domain that is essential for controlling Hippo signaling

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Dlish for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A1ZAY1)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Dlish-RA
FBtr0086860
NM_137407
1402
355
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Dlish-PA
FBpp0086020
40.1
355
4.61
A1ZAY1
NP_611251
AAF57808
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with dachs (via C-terminus); the interaction is direct. Interacts (via N-terminus including SH3 domain 1) with palmitoyltransferase app; this leads to palmitoylation of Dlish by app. Also interacts with dco, ft, ft-regulated E3 ubiquitin ligase Fbxl7, F-box protein slmb and SCF E3 ubiquitin-protein ligase complex component Cul1.

(UniProt, A1ZAY1)
Post Translational Modification

Palmitoylated by app.

(UniProt, A1ZAY1)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dlish using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.07

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | faint | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in apical cortex
inferred from direct assay
(Zhang et al., 2016)
located_in apicolateral plasma membrane
inferred from direct assay
(Misra and Irvine, 2016)
located_in cytoplasm
inferred from direct assay
(Zhang et al., 2016)
located_in subapical part of cell
inferred from direct assay
(Wang et al., 2019)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Dlish in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 12 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Dlish
Transgenic constructs containing regulatory region of Dlish
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Gene disrupted in
Df(2R)Exel7150
(Ryder, 2004.4.26)
Gene partially disrupted in
Df(2R)Snx16Δ1
(Rodal et al., 2011)
Df(2R)Snx16Δ2
(Rodal et al., 2011)
Inferred from location ( 6 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | pupal stage, with Scer\GAL4pnr-MD237
partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237
partially lethal - majority die | recessive
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
Other Phenotypes
Allele
abnormal planar polarity
abnormal planar polarity (with Df(2R)BSC347)
abnormal planar polarity | adult stage
abnormal planar polarity | adult stage, with Scer\GAL4nub.PU
abnormal planar polarity | adult stage (with Df(2R)Exel7150)
abnormal size | adult stage
abnormal size | adult stage, with Scer\GAL4hh-Gal4
abnormal size | adult stage, with Scer\GAL4nub.PU
abnormal size | adult stage (with Df(2R)BSC347)
abnormal size | late third instar larval stage, with Scer\GAL4hh-Gal4
abnormal size | third instar larval stage, with Scer\GAL4en.PU
hyperplasia | adult stage, with Scer\GAL4hh.PU
hypoplasia | adult stage, with Scer\GAL4hh.PU
hypoplasia | adult stage, with Scer\GAL4nub.PU
visible, with Scer\GAL4pnr-MD237
visible | adult stage
visible | adult stage, with Scer\GAL4hh.PU
visible | adult stage, with Scer\GAL4hh-Gal4
visible | adult stage, with Scer\GAL4nub.PU
visible | adult stage (with Df(2R)Exel7150)
Phenotype manifest in
Allele
abdominal tergite marginal bristle
abdominal tergite marginal bristle (with Df(2R)BSC347)
chaeta | increased number, with Scer\GAL4pnr-MD237
crossvein
crossvein (with Df(2R)Exel7150)
crossvein | adult stage
crossvein | adult stage, with Scer\GAL4nub.PU
crossvein | adult stage (with Df(2R)BSC347)
leg
leg (with Df(2R)Exel7150)
mesonotum, with Scer\GAL4pnr-MD237
retina
retina (with Df(2R)Exel7150)
tarsal segment | adult stage
tarsal segment | adult stage, with Scer\GAL4Tub.PU
tarsal segment | adult stage (with Df(2R)BSC347)
trichogen cell, with Scer\GAL4pnr-MD237
wing
wing, with Scer\GAL4nub.PU
wing (with Df(2R)BSC347)
wing blade
wing blade, with Scer\GAL4nub.PU
wing blade (with Df(2R)Exel7150)
wing disc posterior compartment | late third instar larval stage, with Scer\GAL4hh-Gal4
wing disc posterior compartment | third instar larval stage, with Scer\GAL4en.PU
wing hair
wing hair, with Scer\GAL4nub.PU
wing hair (with Df(2R)BSC347)
wing hair (with Df(2R)Exel7150)
wing posterior compartment, with Scer\GAL4hh.PU
wing posterior compartment, with Scer\GAL4hh-Gal4
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (29)
Hsap\SH3D19
2 of 14
Yes
Yes
Hsap\SH3GL1
2 of 14
Yes
No
Hsap\SH3GL2
2 of 14
Yes
No
4  
Hsap\SH3RF3
2 of 14
Yes
No
Hsap\SORBS1
2 of 14
Yes
No
Hsap\SORBS2
2 of 14
Yes
No
Hsap\SORBS3
2 of 14
Yes
No
1  
Hsap\ABL1
1 of 14
No
No
0  
Hsap\ABL2
1 of 14
No
No
2  
Hsap\BLK
1 of 14
No
No
Hsap\FCHSD1
1 of 14
No
No
Hsap\GRAP2
1 of 14
No
No
Hsap\GRAP
1 of 14
No
No
2  
Hsap\GRB2
1 of 14
No
No
Hsap\NCF4
1 of 14
No
Yes
Hsap\NCK1
1 of 14
No
No
2  
Hsap\NEBL
1 of 14
No
No
Hsap\NPHP1
1 of 14
No
Yes
Hsap\SH3GL3
1 of 14
No
No
Hsap\SH3GLB1
1 of 14
No
No
2  
Hsap\SH3GLB2
1 of 14
No
No
1  
Hsap\SH3RF1
1 of 14
No
No
Hsap\SH3RF2
1 of 14
No
No
Hsap\STAC2
1 of 14
No
No
Hsap\STAC3
1 of 14
No
No
Hsap\STAC
1 of 14
No
No
Hsap\TRIO
1 of 14
No
No
1  
Hsap\VAV1
1 of 14
No
No
1  
Hsap\VAV3
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (24)
Rnor\Sorbs2
2 of 14
Yes
No
Rnor\Sorbs3
2 of 14
Yes
No
Rnor\Abl1
1 of 14
No
No
Rnor\Abl2
1 of 14
No
No
Rnor\Blk
1 of 14
No
No
Rnor\Grap2
1 of 14
No
No
Rnor\Grap
1 of 14
No
No
Rnor\Grb2
1 of 14
No
No
Rnor\Kalrn
1 of 14
No
No
Rnor\Ncf4
1 of 14
No
Yes
Rnor\Sh3d19
1 of 14
No
No
Rnor\Sh3gl1
1 of 14
No
No
Rnor\Sh3gl2
1 of 14
No
No
Rnor\Sh3glb1
1 of 14
No
No
Rnor\Sh3glb2
1 of 14
No
No
Rnor\Sh3rf1
1 of 14
No
No
Rnor\Sh3rf2
1 of 14
No
No
Rnor\Sh3rf3
1 of 14
No
No
Rnor\Sorbs1
1 of 14
No
Yes
Rnor\Stac2
1 of 14
No
No
Rnor\Stac3
1 of 14
No
No
Rnor\Stac
1 of 14
No
No
Rnor\Trio
1 of 14
No
No
Rnor\Vav1
1 of 14
No
No
Mus musculus (laboratory mouse) (27)
Mmus\Sh3rf3
2 of 14
Yes
No
Mmus\Sorbs1
2 of 14
Yes
No
Mmus\Sorbs2
2 of 14
Yes
No
Mmus\Sorbs3
2 of 14
Yes
No
Mmus\Abl1
1 of 14
No
No
Mmus\Abl2
1 of 14
No
No
Mmus\Blk
1 of 14
No
No
Mmus\Grap2
1 of 14
No
No
Mmus\Grap
1 of 14
No
No
Mmus\Grb2
1 of 14
No
No
Mmus\Kalrn
1 of 14
No
No
Mmus\Ncf4
1 of 14
No
Yes
Mmus\Nck1
1 of 14
No
No
Mmus\Nphp1
1 of 14
No
Yes
Mmus\Prkcd
1 of 14
No
No
Mmus\Sh3d19
1 of 14
No
No
Mmus\Sh3gl1
1 of 14
No
No
Mmus\Sh3gl2
1 of 14
No
No
Mmus\Sh3glb1
1 of 14
No
No
Mmus\Sh3glb2
1 of 14
No
No
Mmus\Sh3rf1
1 of 14
No
No
Mmus\Sh3rf2
1 of 14
No
No
Mmus\Stac2
1 of 14
No
No
Mmus\Stac3
1 of 14
No
No
Mmus\Stac
1 of 14
No
No
Mmus\Trio
1 of 14
No
No
Mmus\Vav1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (18)
Xtro\abl1
1 of 13
Yes
No
Xtro\abl2
1 of 13
Yes
No
Xtro\enah
1 of 13
Yes
No
Xtro\eogt
1 of 13
Yes
No
Xtro\fchsd1
1 of 13
Yes
No
Xtro\grap
1 of 13
Yes
No
Xtro\grb2
1 of 13
Yes
No
Xtro\mad1l1
1 of 13
Yes
No
Xtro\mir489
1 of 13
Yes
Yes
Xtro\nck1
1 of 13
Yes
No
Xtro\nphp1
1 of 13
Yes
Yes
Xtro\sephs1
1 of 13
Yes
No
Xtro\sh3gl1
1 of 13
Yes
No
Xtro\sh3gl3
1 of 13
Yes
No
Xtro\sod3
1 of 13
Yes
No
Xtro\stac2
1 of 13
Yes
No
Xtro\stac3
1 of 13
Yes
No
Xtro\trio
1 of 13
Yes
No
Danio rerio (Zebrafish) (31)
Drer\sh3rf2
2 of 14
Yes
Yes
Drer\sorbs2a
2 of 14
Yes
No
Drer\abl1
1 of 14
No
No
Drer\abl2
1 of 14
No
No
Drer\asap1a
1 of 14
No
No
Drer\asap1b
1 of 14
No
No
Drer\grap2a
1 of 14
No
No
Drer\grap2b
1 of 14
No
No
Drer\grapa
1 of 14
No
No
Drer\grapb
1 of 14
No
No
Drer\grb2a
1 of 14
No
No
Drer\grb2b
1 of 14
No
No
Drer\itsn1
1 of 14
No
No
Drer\LOC101885672
1 of 14
No
Yes
Drer\ncf4
1 of 14
No
Yes
Drer\nck1b
1 of 14
No
No
Drer\nphp1
1 of 14
No
Yes
Drer\sh3d19
1 of 14
No
No
Drer\sh3gl1a
1 of 14
No
No
Drer\sh3gl1b
1 of 14
No
No
Drer\sh3gl2a
1 of 14
No
No
Drer\sh3glb1a
1 of 14
No
No
Drer\sh3glb1b
1 of 14
No
No
Drer\sh3glb2a
1 of 14
No
No
Drer\sh3glb2b
1 of 14
No
No
Drer\sh3rf1
1 of 14
No
No
Drer\sorbs1
1 of 14
No
No
Drer\sorbs2b
1 of 14
No
No
Drer\stac3
1 of 14
No
No
Drer\stac
1 of 14
No
No
Drer\vav3b
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (7)
Cele\C26C6.8
9 of 14
Yes
Yes
Cele\B0303.7
1 of 14
No
No
Cele\itsn-1
1 of 14
No
No
Cele\nck-1
1 of 14
No
No
Cele\nphp-1
1 of 14
No
Yes
Cele\sem-5
1 of 14
No
No
Cele\unc-57
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (7)
Agam\AgaP_AGAP005616
12 of 12
Yes
Yes
Agam\AgaP_AGAP004766
1 of 12
No
No
Agam\AgaP_AGAP004989
1 of 12
No
No
Agam\AgaP_AGAP006774
1 of 12
No
No
Agam\AgaP_AGAP007717
1 of 12
No
No
Agam\AgaP_AGAP010135
1 of 12
No
No
Agam\AgaP_AGAP011768
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\bzz1
1 of 12
Yes
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Dlish. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (7)
EndoA
2 of 13
CAP
1 of 13
drk
1 of 13
EndoB
1 of 13
nwk
1 of 13
POSH
1 of 13
Stacl
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Dlish - Cul1
    anti tag coimmunoprecipitation, western blot
    (Zhang et al., 2016)
    Dlish - EloC
    anti tag coimmunoprecipitation, anti tag western blot
    (Wang et al., 2019)
    Dlish - Fbxl7
    anti tag coimmunoprecipitation, western blot
    (Zhang et al., 2016)
    Dlish - Zer1
    anti tag coimmunoprecipitation, anti tag western blot
    (Wang et al., 2019)
    Dlish - app
    pull down, western blot, colocalization, fluorescence microscopy, inferred by author, anti tag coimmunoprecipitation, anti tag western blot
    (Matakatsu and Fehon, 2024, Zhang et al., 2016)
    Dlish - dachs
    two hybrid, pull down, anti tag western blot, anti tag coimmunoprecipitation, peptide massfingerprinting
    (Wang et al., 2019, Misra and Irvine, 2016, Zhang et al., 2016, Kwon et al., 2013)
    Dlish - dco
    anti tag coimmunoprecipitation, western blot
    (Zhang et al., 2016)
    Dlish - ds
    anti tag coimmunoprecipitation, western blot, colocalization, fluorescence microscopy, inferred by author, anti tag western blot
    (Matakatsu and Fehon, 2024, Misra and Irvine, 2016, Zhang et al., 2016)
    Dlish - ex
    pull down, anti tag western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry
    (Wang et al., 2019)
    Dlish - ft
    pull down, anti tag western blot, colocalization, fluorescence microscopy, inferred by author, anti tag coimmunoprecipitation, western blot
    (Matakatsu and Fehon, 2024, Fulford et al., 2023, Misra and Irvine, 2016, Zhang et al., 2016)
    Dlish - slmb
    anti tag coimmunoprecipitation, western blot, pull down, anti tag western blot
    (Wang et al., 2019, Zhang et al., 2016)
    Summary of Genetic Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Dlish
    enhanceable
    app
    (Zhang et al., 2016)
    Dlish
    enhanceable
    dachs
    (Misra and Irvine, 2016)
    Dlish
    suppressible
    ex
    (Misra and Irvine, 2016)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    dachs
    enhanceable
    Dlish
    (Misra and Irvine, 2016)
    dachs
    suppressible
    Dlish
    (Zhang et al., 2016)
    ds
    suppressible
    Dlish
    (Misra and Irvine, 2016)
    ft
    suppressible
    Dlish
    (Misra and Irvine, 2016, Zhang et al., 2016)
    ft|ds
    suppressible
    Dlish
    (Matakatsu and Fehon, 2024)
    External Data
    Subunit Structure (UniProtKB)
    Interacts with dachs (via C-terminus); the interaction is direct. Interacts (via N-terminus including SH3 domain 1) with palmitoyltransferase app; this leads to palmitoylation of Dlish by app. Also interacts with dco, ft, ft-regulated E3 ubiquitin ligase Fbxl7, F-box protein slmb and SCF E3 ubiquitin-protein ligase complex component Cul1.
    (UniProt, A1ZAY1 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyBase
    External Links
    External Data
    Linkouts
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-84
    Cytogenetic map
    54E2-54E2
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    54E2-54E2
    Limits computationally determined from genome sequence between P{lacW}l(2)k07509k07509&P{EP}CG10934EP538 and P{EP}subEP616
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-84

    (FlyBase, 2016)

    2-86.8

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (5)
    Genomic Clones (21)
    cDNA Clones (21)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Zhang et al., 2016)
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments

        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

        (Kiger et al., 2003)
        Relationship to Other Genes
        Source for database merge of
        Additional comments
        Nomenclature History
        Source for database identify of

        Source for identity of: Dlish CG10933

        (Zhang et al., 2016)
        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (7)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 33 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        FlyMine - An integrated database for Drosophila genomics
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        Flygut - An atlas of the Drosophila adult midgut
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        MIST (protein-protein) - An integrated Molecular Interaction Database
        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
        References (46)