Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
17 of 26 hits represented clones of Dlish.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of CME-W1-Cl.8+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; bait produced by in vitro translation; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced by in vitro translation; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected cell extract.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
