FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Dlish, ex
FlyBase ID
FBig0000117691
Interaction Network
Interactions Browser links
Dlish network
ex network
Reported Interactions
FBrf0241288-1.coIP.WB
Reference
Wang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

FLAG tag

ex 
ex 
ex 
Dlish 

Dlish

prey

V5 tag

Dlish 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
C2 domain
sufficient binding region
aa 912-1164
ex 
C3 domain
sufficient binding region
aa 1168-1427
ex 
SH3bd-3 domain
necessary binding region
unspecified
Dlish 
SH3-2,3 domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2019)
FBrf0241288-12.coIP.MS
Reference
Wang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Dlish 

Dlish

bait

FLAG tag

ex 

Ex

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2019)
FBrf0241288-2.coIP.WB
Reference
Wang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Dlish 

Dlish

bait

V5 tag

ex 

Ex

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2019)
FBrf0241288-3.coIP.WB
Reference
Wang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ex 

Ex

bait

FLAG tag

ex 
Dlish 

Dlish

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ex 
C3 domain
sufficient binding region
aa 1168-1427
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro translation; prey produced as a recombinant fusion protein in bacterial system.

(Wang et al., 2019)
FBrf0241288-4.PD.WB
Reference
Wang et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Dlish 

Dlish

bait

GST tag

Dlish 
Dlish 
ex 

Ex

prey

FLAG or His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dlish 
SH3-2 domain
sufficient binding region
unspecified
Dlish 
SH3-3 domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Wang et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)