FlyBase Allele Report: Dmel\dachs[UAS.Tag:V5,Tag:polyHis]

General Information

Symbol

Dmel\dachs^{UAS.Tag:V5,Tag:polyHis}

Species

D. melanogaster

Name

FlyBase ID

FBal0193610

Feature type

allele

Associated gene

Dmel\dachs

Associated Insertion(s)

Carried in Construct

P{UAS-dachs.V5}

Also Known As

UAS-Dachs:V5, UAS-d:v5, UAS-Dachs-V5

Key Links

Transgenic product class

wild_type

(Mao et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Mao et al., 2006)

Mutagen

in vitro construct

(Mao et al., 2006)

Progenitor genotype

dachs^UAS.cMa

(Mao et al., 2006)

Carried in construct

P{UAS-dachs.V5}

Cytology

Description

Tag:V5 and Tag:polyHis epitopes have replaced the stop codon and 3' UTR of d.

(Mao et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

abnormal size | adult stage, with Scer\GAL4^en-e16E

(Poon et al., 2011)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

hyperplasia | adult stage, with Scer\GAL4^hh.PU

(Zhang et al., 2016)

hyperplasia | adult stage, with Scer\GAL4^Tub.PU

(Zhang et al., 2016)

visible, with Scer\GAL4^Tub.PU

(Mao et al., 2006)

visible | adult stage, with Scer\GAL4^en-e16E

(Poon et al., 2011)

visible | adult stage, with Scer\GAL4^hh.PU

(Zhang et al., 2016)

visible | adult stage, with Scer\GAL4^Tub.PU

(Zhang et al., 2016)

Phenotype Manifest In

crossvein, with Scer\GAL4^Tub.PU

(Mao et al., 2006)

leg, with Scer\GAL4^Tub.PU

(Mao et al., 2006)

wing, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

wing, with Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

wing, with Scer\GAL4^Tub.PU

(Mao et al., 2006)

wing | posterior, with Scer\GAL4^en-e16E

(Poon et al., 2011)

wing blade, with Scer\GAL4^Tub.PU

(Zhang et al., 2016)

wing posterior compartment, with Scer\GAL4^hh.PU

(Zhang et al., 2016)

Detailed Description

Statement

Reference

The expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^nub.PU leads to a small but significant increase in the adult wing area, as compared to controls.

(Misra and Irvine, 2016)

Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of either Scer\GAL4^tub.PU or Scer\GAL4^hh.PU induces overgrowth of either the whole adult wing blade or of the posterior compartment only, respectively.

(Zhang et al., 2016)

Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^en-e16E results in wing overgrowth.

(Poon et al., 2011)

Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} (together with Dcr-2^Scer\UAS.cDa) using Scer\GAL4^nub-AC-62 results in overgrown wings.

(Rauskolb et al., 2011)

Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^tub has no effect on planar cell polarity in the adult abdomen.

(Matakatsu and Blair, 2008)

Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^tub results in increased wing size and crossvein abnormalities and also induces tissue outgrowth in the leg.

(Mao et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, dachs^{UAS.Tag:V5,Tag:polyHis} has abnormal size | adult stage phenotype, enhanceable by Dlish^{UAS.Tag:FLAG,TagRFP}, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Scer\GAL4^nub-AC-62, dachs^{UAS.Tag:V5,Tag:polyHis} has abnormal size phenotype, enhanceable by Scer\GAL4^nub-AC-62/Zyx^UAS.Tag:V5

(Rauskolb et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, dachs^{UAS.Tag:V5,Tag:polyHis} has visible | adult stage phenotype, suppressible by Dlish⁴⁵⁰⁶/Dlish⁴⁵⁰⁶

(Zhang et al., 2016)

Scer\GAL4^hh.PU, dachs^{UAS.Tag:V5,Tag:polyHis} has hyperplasia | adult stage phenotype, suppressible by Dlish⁴⁵⁰⁶/Dlish⁴⁵⁰⁶

(Zhang et al., 2016)

Scer\GAL4^en-e16E, dachs^{UAS.Tag:V5,Tag:polyHis} has abnormal size | adult stage phenotype, suppressible by Scer\GAL4^en-e16E/Tao^{UASp.Tag:FLAG}

(Poon et al., 2011)

Scer\GAL4^en-e16E, dachs^{UAS.Tag:V5,Tag:polyHis} has visible | adult stage phenotype, suppressible by Scer\GAL4^en-e16E/Tao^{UASp.Tag:FLAG}

(Poon et al., 2011)

Scer\GAL4^nub-AC-62, dachs^{UAS.Tag:V5,Tag:polyHis} has abnormal size phenotype, suppressible by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

Enhancer of

Statement

Reference

dachs^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^nub.PU is an enhancer of abnormal size | adult stage phenotype of Dlish^{UAS.Tag:FLAG,TagRFP}, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Scer\GAL4^nub-AC-62/dachs^{UAS.Tag:V5,Tag:polyHis} is an enhancer of abnormal size phenotype of Scer\GAL4^nub-AC-62, Zyx^UAS.Tag:V5

(Rauskolb et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/dachs^{UAS.Tag:V5,Tag:polyHis} is a suppressor of abnormal planar polarity phenotype of app^e6

(Matakatsu and Blair, 2008)

Other

Statement

Reference

Scer\GAL4^Tub.PU, app^RA.UAS, dachs^{UAS.Tag:V5,Tag:polyHis} has abnormal planar polarity phenotype

(Matakatsu and Blair, 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, dachs^{UAS.Tag:V5,Tag:polyHis} has wing phenotype, enhanceable by Dlish^{UAS.Tag:FLAG,TagRFP}, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Scer\GAL4^nub-AC-62, dachs^{UAS.Tag:V5,Tag:polyHis} has wing phenotype, enhanceable by Scer\GAL4^nub-AC-62/Zyx^UAS.Tag:V5

(Rauskolb et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^hh.PU, dachs^{UAS.Tag:V5,Tag:polyHis} has wing posterior compartment phenotype, suppressible by Dlish⁴⁵⁰⁶/Dlish⁴⁵⁰⁶

(Zhang et al., 2016)

Scer\GAL4^en-e16E, dachs^{UAS.Tag:V5,Tag:polyHis} has wing | posterior phenotype, suppressible by Scer\GAL4^en-e16E/Tao^{UASp.Tag:FLAG}

(Poon et al., 2011)

Scer\GAL4^nub-AC-62, dachs^{UAS.Tag:V5,Tag:polyHis} has wing phenotype, suppressible by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

Enhancer of

Statement

Reference

dachs^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^nub.PU is an enhancer of wing phenotype of Dlish^{UAS.Tag:FLAG,TagRFP}, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Scer\GAL4^nub-AC-62/dachs^{UAS.Tag:V5,Tag:polyHis} is an enhancer of wing phenotype of Scer\GAL4^nub-AC-62, Zyx^UAS.Tag:V5

(Rauskolb et al., 2011)

Other

Statement

Reference

Scer\GAL4^Tub.PU, app^RA.UAS, dachs^{UAS.Tag:V5,Tag:polyHis} has adult abdomen phenotype

(Matakatsu and Blair, 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} and Dlish^{Scer\UAS.T:Zzzz\FLAG,T:Equa\eqFP578-TagRFP} exacerbates the modest increase in wing area resulting from the single expression of either d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} or Dlish^{Scer\UAS.T:Zzzz\FLAG,T:Equa\eqFP578-TagRFP}.

(Misra and Irvine, 2016)

The overgrowth of the posterior wing compartment observed in adults expressing d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^hh.PU is fully suppressed by combination with Dlish⁴⁵⁰⁶ in homozygous state.

(Zhang et al., 2016)

Expression of Tao^{Scer\UAS.P\T.T:Zzzz\FLAG} reduces the wing overgrowth seen when d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} is expressed under the control of Scer\GAL4^en-e16E.

(Poon et al., 2011)

The wing overgrowth phenotype associated with Scer\GAL4^nub-AC-62-mediated expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} (together with Dcr-2^Scer\UAS.cDa) is suppressed by co-expression of Zyx102EF^NIG.32018R.

Over-expression of Zyx102EF^{Scer\UAS.T:SV5\V5} (together with Dcr-2^Scer\UAS.cDa) using Scer\GAL4^nub-AC-62 synergizes with d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} over-expression to result in enhanced wing overgrowth in adult flies.

(Rauskolb et al., 2011)

Co-expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} and app^RA.Scer\UAS under the control of Scer\GAL4^tub results in planar cell polarity defects in the adult abdomen.

The PCP defects that are seen between distal veins L4 and L5 in app^e6 mutants are rescued on the dorsal surface by expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^ap-md544.

(Matakatsu and Blair, 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

dachs^{UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^Tub.PU rescues dachs^GC13

(Zhang et al., 2016)

Partially rescues

dachs^{UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^Tub.PU partially rescues dachs^GC13

(Mao et al., 2006)

Comments

Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^tub.PU restores the viability of d^GC13 mutants.

(Zhang et al., 2016)

Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^tub provides significant rescue of the wing and leg phenotypes of d^GC13 mutants.

(Mao et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

28291

w^*; P{UAS-dachs.V5}9-F

28814

w^*; P{UAS-dachs.V5}50-5/CyO

Notes on Origin

Discoverer