FlyBase Allele Report: Dmel\Dlish[HMS01708]

General Information

Symbol

Dmel\Dlish^HMS01708

Species

D. melanogaster

Name

FlyBase ID

FBal0278826

Feature type

allele

Associated gene

Dmel\Dlish

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01708}

Also Known As

UAS-vam-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01708}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01708

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | adult stage, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

hypoplasia | adult stage, with Scer\GAL4^hh.PU

(Zhang et al., 2016)

hypoplasia | adult stage, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

visible | adult stage, with Scer\GAL4^hh.PU

(Zhang et al., 2016)

visible | adult stage, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

Phenotype Manifest In

crossvein | adult stage, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

tarsal segment | adult stage, with Scer\GAL4^Tub.PU

(Misra and Irvine, 2016)

wing, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

wing blade, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

wing hair, with Scer\GAL4^nub.PU

(Zhang et al., 2016)

wing posterior compartment, with Scer\GAL4^hh.PU

(Zhang et al., 2016)

Detailed Description

Statement

Reference

The expression of Dlish^HMS01708 under the combined control of Scer\GAL4^nub.PU and Dicer-2 transgene (for an efficient RNAi) leads to significant decreases in total wing area and in wing inter-crossvein distance, and leads to mostly normal orientation of the wing hairs, as compared to controls; expression under the control of Scer\GAL4^tub.PU leads to a significant decrease in tarsal length, as compared to controls.

(Misra and Irvine, 2016)

Expression of Dlish^HMS01708 reduces the size of the posterior wing compartment (driven by Scer\GAL4^hh.PU) or the whole adult wing blade and causes hair polarity defects (driven by Scer\GAL4^nub.PU) relative to controls.

(Zhang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dlish^HMS01708, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, suppressible by ex^GD12782, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

NOT suppressed by

Statement

Reference

Dlish^HMS01708, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, non-suppressible by ft^GD881, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Dlish^HMS01708, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, non-suppressible by ds^GD2646, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Suppressor of

Statement

Reference

Dlish^HMS01708, Scer\GAL4^nub.PU is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, ds^GD2646

(Misra and Irvine, 2016)

Dlish^HMS01708, Scer\GAL4^nub.PU is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, ft^GD881

(Misra and Irvine, 2016)

Dlish^HMS01708, Scer\GAL4^nub.PU is a suppressor of abnormal planar polarity | adult stage phenotype of Scer\GAL4^nub.PU, ft^GD881

(Misra and Irvine, 2016)

NOT Suppressor of

Statement

Reference

Dlish^HMS01708, Scer\GAL4^nub.PU is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, ex^GD12782

(Misra and Irvine, 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dlish^HMS01708, Scer\GAL4^nub.PU has wing phenotype, suppressible by ex^GD12782, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

NOT suppressed by

Statement

Reference

Dlish^HMS01708, Scer\GAL4^nub.PU has wing phenotype, non-suppressible by ft^GD881, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Dlish^HMS01708, Scer\GAL4^nub.PU has wing phenotype, non-suppressible by ds^GD2646, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Suppressor of

Statement

Reference

Dlish^HMS01708, Scer\GAL4^nub.PU is a suppressor of wing phenotype of Scer\GAL4^nub.PU, ft^GD881

(Misra and Irvine, 2016)

Dlish^HMS01708, Scer\GAL4^nub.PU is a suppressor of wing hair phenotype of Scer\GAL4^nub.PU, ft^GD881

(Misra and Irvine, 2016)

Dlish^HMS01708, Scer\GAL4^nub.PU is a suppressor of wing phenotype of Scer\GAL4^nub.PU, ds^GD2646

(Misra and Irvine, 2016)

NOT Suppressor of

Statement

Reference

Dlish^HMS01708, Scer\GAL4^nub.PU is a non-suppressor of wing phenotype of Scer\GAL4^nub.PU, ex^GD12782

(Misra and Irvine, 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Dlish^HMS01708 and either ft^GD881 or ds^GD2646 under the combined control of Scer\GAL4^nub.PU and Dicer-2 transgene (for an efficient RNAi) results in wing phenotypes that are similar to those observed upon expression of Dlish^HMS01708 alone (i.e. small wings and virtually no wing hair orientation defects), instead of similar to those observed upon expression of ft^GD881 alone (i.e. big wing area and severe wing hair orientation defects) or ds^GD2646 alone (i.e. big wing area). Conversely, the co-expression of ex^GD12782 and Dlish^HMS01708 under the combined control of Scer\GAL4^nub.PU and Dicer-2 transgene (for an efficient RNAi) results in a big wing area phenotype similar to that observed upon expression of ex^GD12782 alone.

(Misra and Irvine, 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38263

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01708}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG10933^HMS01708

Dlish^HMS01708

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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