FlyBase Allele Report: Dmel\ex[GD12782]

General Information

Symbol

Dmel\ex^GD12782

Species

D. melanogaster

Name

FlyBase ID

FBal0209531

Feature type

allele

Associated gene

Dmel\ex

Associated Insertion(s)

Carried in Construct

P{GD12782}

Also Known As

UAS-ex RNAi, UAS-ex-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12782}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12782

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

abnormal size | adult stage, with Scer\GAL4^en-e16E

(Poon et al., 2011)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

increased cell number | pupal stage, with Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^ey.PU

(Chung et al., 2016)

visible, with Scer\GAL4^GMR.PU

(Genevet et al., 2010)

visible, with Scer\GAL4^hh-Gal4

(Huang et al., 2013)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^en-e16E

(Poon et al., 2011)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PU

(Chung et al., 2016)

eye, with Scer\GAL4^GMR.PU

(Genevet et al., 2010)

interommatidial cell | pupal stage | increased number, with Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Huang et al., 2013)

wing, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

wing, with Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

wing | posterior, with Scer\GAL4^en-e16E

(Poon et al., 2011)

wing disc, with Scer\GAL4^hh-Gal4

(Huang et al., 2013)

Detailed Description

Statement

Reference

Expression of ex^GD12782 driven by Scer\GAL4^ey.PU leads to a small eye phenotype.

(Chung et al., 2016)

The expression of ex^GD12782 under the combined control of Scer\GAL4^nub.PU and Dicer-2 transgene (for an efficient RNAi) leads to a significant increase in total wing area, as compared to controls.

(Misra and Irvine, 2016)

Wing discs expressing ex^GD12782 under the control of Scer\GAL4^hh-Gal4 have an increased P-compartment size compared to controls. Expression of ex^GD12782 under the control of Scer\GAL4^Bx-MS1096 results in wing overgrowth.

(Huang et al., 2013)

Expression of ex^GD12782 under the control of Scer\GAL4^GMR.PFa results in a small increase in the number of inter-ommatidial cells in the pupal eye.

(Gilbert et al., 2011)

Expression of ex^GD12782 under the control of Scer\GAL4^en-e16E results in wing overgrowth.

(Poon et al., 2011)

Expression of ex^GD12782 using Scer\GAL4^nub-AC-62 results in overgrown wings.

(Rauskolb et al., 2011)

Expression of ex^GD12782 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^repo.PU has no effect on glial cell number in the third instar larval eye disc.

(Reddy and Irvine, 2011)

Expression of ex^GD12782 under the control of Scer\GAL4^GMR.PF has no effect on eye size.

(Baumgartner et al., 2010)

Expression of ex^GD12782 under the control of Scer\GAL4^GMR.PF results in eye overgrowth.

(Genevet et al., 2010)

Adults expressing ex^GD12782 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12782

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, ex^GD12782 has increased cell number | pupal stage phenotype, enhanceable by mop^KK100235/Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, ex^GD12782 has visible phenotype, suppressible by Schip1^UAS.cCa/Scer\GAL4^ey.PU

(Chung et al., 2016)

ex^GD12782 has visible phenotype, suppressible by par-1^HMS00405/Scer\GAL4^Bx-MS1096

(Huang et al., 2013)

Scer\GAL4^en-e16E, ex^GD12782 has abnormal size | adult stage phenotype, suppressible by Scer\GAL4^en-e16E/Tao^{UASp.Tag:FLAG}

(Poon et al., 2011)

Scer\GAL4^en-e16E, ex^GD12782 has visible | adult stage phenotype, suppressible by Scer\GAL4^en-e16E/Tao^{UASp.Tag:FLAG}

(Poon et al., 2011)

Scer\GAL4^nub-AC-62, ex^GD12782 has abnormal size phenotype, suppressible | partially by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

Scer\GAL4^nub-AC-62, ex^GD12782 has abnormal size phenotype, suppressible by Scer\GAL4^nub-AC-62/jub^GD7185

(Rauskolb et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^nub.PU, ex^GD12782 has abnormal size | adult stage phenotype, non-suppressible by Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Enhancer of

Statement

Reference

ex^GD12782/Scer\GAL4^GMR.PFa is an enhancer of increased cell number | pupal stage phenotype of Scer\GAL4^GMR.PFa, mop^KK100235

(Gilbert et al., 2011)

ex^GD12782/Scer\GAL4^GMR.PU is an enhancer of visible | somatic clone phenotype of kibra¹

(Baumgartner et al., 2010)

Suppressor of

Statement

Reference

ex^GD12782, Scer\GAL4^nub.PU is a suppressor of abnormal size | adult stage phenotype of Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Other

Statement

Reference

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)}, ex^GD12782 has visible | adult stage phenotype

(Gaspar et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, ex^GD12782 has interommatidial cell | pupal stage | increased number phenotype, enhanceable by mop^KK100235/Scer\GAL4^GMR.PFa

(Gilbert et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, ex^GD12782 has eye phenotype, suppressible by Schip1^UAS.cCa/Scer\GAL4^ey.PU

(Chung et al., 2016)

Scer\GAL4^Bx-MS1096, ex^GD12782 has wing phenotype, suppressible by par-1^HMS00405/Scer\GAL4^Bx-MS1096

(Huang et al., 2013)

Scer\GAL4^hh-Gal4, ex^GD12782 has wing disc phenotype, suppressible by par-1^HMS00405/Scer\GAL4^hh-Gal4

(Huang et al., 2013)

Scer\GAL4^en-e16E, ex^GD12782 has wing | posterior phenotype, suppressible by Scer\GAL4^en-e16E/Tao^{UASp.Tag:FLAG}

(Poon et al., 2011)

Scer\GAL4^nub-AC-62, ex^GD12782 has wing phenotype, suppressible | partially by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

Scer\GAL4^nub-AC-62, ex^GD12782 has wing phenotype, suppressible by Scer\GAL4^nub-AC-62/jub^GD7185

(Rauskolb et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^nub.PU, ex^GD12782 has wing phenotype, non-suppressible by Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Enhancer of

Statement

Reference

ex^GD12782/Scer\GAL4^GMR.PFa is an enhancer of interommatidial cell | pupal stage | increased number phenotype of Scer\GAL4^GMR.PFa, mop^KK100235

(Gilbert et al., 2011)

ex^GD12782/Scer\GAL4^GMR.PU is an enhancer of eye | somatic clone phenotype of kibra¹

(Baumgartner et al., 2010)

Suppressor of

Statement

Reference

ex^GD12782, Scer\GAL4^nub.PU is a suppressor of wing phenotype of Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Other

Statement

Reference

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)}, ex^GD12782 has eye disc | third instar larval stage phenotype

(Gaspar et al., 2015)

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)}, ex^GD12782 has eye | adult stage phenotype

(Gaspar et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Schip1^Scer\UAS.cCa suppresses the small eye phenotype seen in flies with expression of ex^GD12782 driven by Scer\GAL4^ey.PU.

(Chung et al., 2016)

The co-expression of ex^GD12782 and Dlish^HMS01708 under the combined control of Scer\GAL4^nub.PU and Dicer-2 transgene (for an efficient RNAi) results in a big wing area phenotype similar to that observed upon expression of ex^GD12782 alone, instead of the small wing area phenotype observed upon the expression of Dlish^HMS01708 alone.

(Misra and Irvine, 2016)

Co-expression of ex^GD12782 and Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^GMR.PU results in reduced adult eye size and a complete loss of photoreceptor cells in third instar larval eye discs compared to when ex^GD12782 is expressed alone.

Co-expression of d^Scer\UAS.cUa and ex^GD12782 under the control of Scer\GAL4^GMR.PU does not significantly reduce adult eye size or the number of photoreceptor cells in third instar larval eye discs.

(Gaspar et al., 2015)

Expression of par-1^HMS00405 suppresses the increased P-compartment phenotype seen in wing discs when ex^GD12782 is expressed under the control of Scer\GAL4^hh-Gal4.

Expression of par-1^HMS00405 suppresses the wing overgrowth seen when ex^GD12782 is expressed under the control of Scer\GAL4^Bx-MS1096.

(Huang et al., 2013)

Co-expression of ex^GD12782 and mop^KK100235 under the control of Scer\GAL4^GMR.PFa enhances the increase in pupal inter-ommatidial cells seen when either transgene is expressed alone.

(Gilbert et al., 2011)

Expression of Tao^{Scer\UAS.P\T.T:Zzzz\FLAG} reduces the wing overgrowth seen when ex^GD12782 is expressed under the control of Scer\GAL4^en-e16E.

(Poon et al., 2011)

The wing overgrowth phenotype associated with Scer\GAL4^nub-AC-62-mediated expression of ex^GD12782 (together with Dcr-2^Scer\UAS.cDa) is partially suppressed by co-expression of Zyx102EF^NIG.32018R, and is suppressed by co-expression of jub^GD7185.

(Rauskolb et al., 2011)

Expression of ex^GD12782 under the control of Scer\GAL4^GMR.PF severely enhances the eye overgrowth phenotype of homozygous kibra¹ eyes (generated using the eyFLP method).

(Baumgartner et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v22994

w¹¹¹⁸; P{GD12782}v22994

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12782

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ex^GD12782

Name Synonyms

Secondary FlyBase IDs

References (24)

Report Sections

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