FlyBase Allele Report: Dmel\Zyx[UAS.Tag:Myr(Src42A)]

General Information

Symbol

Dmel\Zyx^{UAS.Tag:Myr(Src42A)}

Species

D. melanogaster

Name

FlyBase ID

FBal0319349

Feature type

allele

Associated gene

Dmel\Zyx

Associated Insertion(s)

Carried in Construct

PBac{UAS-Zyx.N-myr}

Key Links

Transgenic product class

cDNA

(Gaspar et al., 2015)

wild_type

(Gaspar et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Gaspar et al., 2015)

wild_type

(Gaspar et al., 2015)

Mutagen

in vitro construct

(Gaspar et al., 2015)

Progenitor genotype

Carried in construct

PBac{UAS-Zyx.N-myr}

Cytology

Description

UAS regulatory sequences drive expression of the Zyx LD06023 cDNA, that is tagged at the N-terminal end with the Tag:Myr(Src42A) myristoylation signal.

(Gaspar et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Zyx

Tagged with

Tag:Myr(Src42A)

membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | third instar larval stage, with Scer\GAL4^hh.PU

(Gaspar et al., 2015)

hyperplasia, with Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

hyperplasia, with Scer\GAL4^nub.PU

(Gaspar et al., 2015)

increased cell number | adult stage, with Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

increased cell number | pupal stage, with Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

visible, with Scer\GAL4^nub.PU, Zyx^Δ41

(Gaspar et al., 2015)

visible | adult stage, with Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

visible | adult stage, with Scer\GAL4^nub.PU

(Gaspar et al., 2015)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

interommatidial cell | pupal stage | increased number, with Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

wing blade, with Scer\GAL4^nub.PU

(Gaspar et al., 2015)

wing blade, with Scer\GAL4^nub.PU, Zyx^Δ41

(Gaspar et al., 2015)

wing disc | third instar larval stage, with Scer\GAL4^hh.PU

(Gaspar et al., 2015)

Detailed Description

Statement

Reference

Ectopic expression of Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^nub.PU results in significantly larger wings in adult flies and expression under Scer\GAL4^GMR.PU results in significantly increased number of inter-ommatidial cells in the pupal retina and overgrown eyes in adults compared to controls.

Ectopic expression of Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^hh.PU increases cell proliferation in third instar larval wing disc epithelium and results in higher posterior/anterior compartment ratio compared to controls.

Ectopic expression of Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^GMR.PU results in significantly increased number of inter-ommatidial cells in pupal retina compared to wild-type.

Flies expressing Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^nub.PU in a Zyx^Δ41/Zyx^Δ41 background have adult wings that are significantly bigger in size than wild-type.

(Gaspar et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)} has visible | adult stage phenotype, enhanceable by ena^UAS.cGb/Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^nub.PU, Zyx^{UAS.Tag:Myr(Src42A)} has visible | adult stage phenotype, suppressible | partially by cpb^UAS.cWa/cpa^UAS.Tag:HA/Scer\GAL4^nub.PU

(Gaspar et al., 2015)

Scer\GAL4^nub.PU, Zyx^{UAS.Tag:Myr(Src42A)} has hyperplasia phenotype, suppressible | partially by cpb^UAS.cWa/cpa^UAS.Tag:HA/Scer\GAL4^nub.PU

(Gaspar et al., 2015)

Suppressor of

Statement

Reference

Zyx^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, ex^UAS.cBa

(Gaspar et al., 2015)

Other

Statement

Reference

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)}, ex^GD12782 has visible | adult stage phenotype

(Gaspar et al., 2015)

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)}, dachs^UAS.cUa has visible | adult stage phenotype

(Gaspar et al., 2015)

Scer\GAL4^nub.PU, Zyx^{UAS.Tag:Myr(Src42A)}, ena^UAS.cGb has visible | adult stage phenotype

(Gaspar et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)} has eye | adult stage phenotype, enhanceable by ena^UAS.cGb/Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)} has interommatidial cell | pupal stage | increased number phenotype, enhanceable by ena^UAS.cGb/Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)} has interommatidial cell | pupal stage phenotype, enhanceable by cpb^UAS.cWa/cpa^{ΔABD.UAS.Tag:HA}/Scer\GAL4^GMR.PU

(Gaspar et al., 2015)

Suppressed by

Statement

Reference

Zyx^{UAS.Tag:Myr(Src42A)} has wing blade phenotype, suppressible | partially by cpb^UAS.cWa/cpa^UAS.Tag:HA/Scer\GAL4^nub.PU

(Gaspar et al., 2015)

Suppressor of

Statement

Reference

Zyx^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^GMR.PU is a suppressor | partially of eye | adult stage phenotype of Scer\GAL4^GMR.PU, ex^UAS.cBa

(Gaspar et al., 2015)

Scer\GAL4^nub.PU/Zyx^{UAS.Tag:Myr(Src42A)} is a suppressor of wing blade phenotype of Scer\GAL4^nub.PU, cpa^UAS.Tag:HA, cpb^UAS.cWa

(Gaspar et al., 2015)

Other

Statement

Reference

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)}, ex^GD12782 has eye disc | third instar larval stage phenotype

(Gaspar et al., 2015)

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)}, ex^GD12782 has eye | adult stage phenotype

(Gaspar et al., 2015)

Scer\GAL4^nub.PU, Zyx^{UAS.Tag:Myr(Src42A)}, cpa^{ΔABD.UAS.Tag:HA}, cpb^UAS.cWa has wing blade phenotype

(Gaspar et al., 2015)

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)}, dachs^UAS.cUa has eye disc | third instar larval stage phenotype

(Gaspar et al., 2015)

Scer\GAL4^GMR.PU, Zyx^{UAS.Tag:Myr(Src42A)}, dachs^UAS.cUa has eye | adult stage phenotype

(Gaspar et al., 2015)

Scer\GAL4^nub.PU, Zyx^{UAS.Tag:Myr(Src42A)}, ena^UAS.cGb has wing blade phenotype

(Gaspar et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Zyx^{Scer\UAS.T:Myr-Src42A} and ena^Scer\UAS.cGb under the control of Scer\GAL4^GMR.PU results in increased adult eye size and significantly increased number of inter-ommatidial cells in the pupal retina compared to when either ena^Scer\UAS.cGb or Zyx^{Scer\UAS.T:Myr-Src42A} is expressed alone.

The increased adult wing size due to expression of Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^nub.PU can be partially suppressed by co-expression of cpa^{Scer\UAS.T:Ivir\HA1} and cpb^Scer\UAS.cWa.

Co-expression of Zyx^{Scer\UAS.T:Myr-Src42A} and cpa^{ΔABD.Scer\UAS.T:Ivir\HA1} and cpb^Scer\UAS.cWa under the control of Scer\GAL4^nub.PU results in severely malformed adult wings that are not observed when either Zyx^{Scer\UAS.T:Myr-Src42A} is expressed alone or cpa^{ΔABD.Scer\UAS.T:Ivir\HA1} is co-expressed with cpb^Scer\UAS.cWa only.

The supernumerary inter-ommatidial cells in the pupal retina phenotype characteristic for animals expressing Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^GMR.PU can be enhanced by co-expression with cpa^{ΔABD.Scer\UAS.T:Ivir\HA1} and cpb^Scer\UAS.cWa.

The small eye phenotype seen in adult flies expressing ex^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PU can be strongly alleviated by co-expression of Zyx^{Scer\UAS.T:Myr-Src42A}.

Co-expression of ex^GD12782 and Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^GMR.PU results in reduced adult eye size and a complete loss of photoreceptor cells in third instar larval eye discs compared to when ex^GD12782 is expressed alone.

Co-expression of d^Scer\UAS.cUa and Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^GMR.PU results in reduced adult eye size and severely decreased number of photoreceptor cells in third instar larval eye discs compared to when d^Scer\UAS.cUa is expressed alone.

Co-expression of ena^Scer\UAS.cGb and Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^nub.PU results in severely malformed adult wings that are not observed when either ena^Scer\UAS.cGb or Zyx^{Scer\UAS.T:Myr-Src42A} is expressed alone.

(Gaspar et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^nub.PU/Zyx^{UAS.Tag:Myr(Src42A)} rescues Zyx^Δ41

(Gaspar et al., 2015)

Comments

The small wing phenotype displayed by Zyx^Δ41/Zyx^Δ41 adults can be fully rescued by expression of Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^nub.PU in the mutant background, resulting in adult wings that are significantly bigger in size than wild-type.

(Gaspar et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Zyx^{Scer\UAS.T:Myr-Src42A}

Zyx^{UAS.Tag:Myr(Src42A)}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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