FlyBase Allele Report: Dmel\Zyx[Δ41]

General Information

Symbol

Dmel\Zyx^Δ41

Species

D. melanogaster

Name

FlyBase ID

FBal0319347

Feature type

allele

Associated gene

Dmel\Zyx

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

TALEN

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Gaspar et al., 2015)

Mutagen

TALEN

(Gaspar et al., 2015)

Progenitor genotype

Cytology

Description

7bp deletion which introduces a frameshift at codon 103 (isoforms RA and RB), 41 (isoform RG), or 14 (isoform RH), resulting in a premature stop codon leading to a 19 to 108 amino acid residue peptide that lacks most of the wild-type Zyx sequence.

(Gaspar et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

4:1,059,714..1,059,720 [-]

Comment:

Seven bp deletion introduces a frameshift at codon 103 of Zyx-PA, Zyx-PB and Zyx-PL.

(Gaspar et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | adult stage

(Gaspar et al., 2015)

fertile

(Gaspar et al., 2015)

viable

(Gaspar et al., 2015)

visible, with Scer\GAL4^nub.PU, Zyx^{UAS.Tag:Myr(Src42A)}

(Gaspar et al., 2015)

visible | adult stage | recessive

(Gaspar et al., 2015)

Phenotype Manifest In

wing blade

(Gaspar et al., 2015)

wing blade, with Scer\GAL4^nub.PU, Zyx^{UAS.Tag:Myr(Src42A)}

(Gaspar et al., 2015)

Detailed Description

Statement

Reference

Zyx^Δ41/Zyx^Δ41 adult flies are smaller and lighter compared to wild-type and have smaller and narrower wings but do not display any defects in eye development (number od inter-ommatidial cells in the pupal retina, photoreceptor differentiation as well as adult eye size are normal).

(Gaspar et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Zyx^Δ41/Zyx^Δ41 is an enhancer of visible | adult stage phenotype of dachs^GC13

(Gaspar et al., 2015)

Suppressor of

Statement

Reference

Zyx^Δ41/Zyx^Δ41 is a suppressor of visible | adult stage phenotype of ex^e1

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a suppressor of lethal phenotype of ex^e1

(Gaspar et al., 2015)

NOT Suppressor of

Statement

Reference

Zyx^Δ41/Zyx^Δ41 is a non-suppressor of lethal phenotype of ft⁸/ft^G-rv

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a non-suppressor of visible | adult stage phenotype of hpo^5.1

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a non-suppressor of visible | adult stage phenotype of ft^G-rv

(Gaspar et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Zyx^Δ41/Zyx^Δ41 is an enhancer of eye disc | third instar larval stage phenotype of dachs¹/dachs^GC13

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is an enhancer of wing disc | third instar larval stage phenotype of dachs¹/dachs^GC13

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is an enhancer of eye | adult stage | somatic clone phenotype of dachs^GC13

(Gaspar et al., 2015)

Suppressor of

Statement

Reference

Zyx^Δ41/Zyx^Δ41 is a suppressor of eye | somatic clone | adult stage phenotype of ex^e1

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a suppressor of interommatidial cell | increased number | somatic clone | pupal stage phenotype of ex^e1

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a suppressor of eye disc | third instar larval stage phenotype of ex^e1

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a suppressor of interommatidial cell | increased number | somatic clone | pupal stage phenotype of ft^G-rv

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a suppressor of wing disc | third instar larval stage phenotype of ex^AP50/ex^e1

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a suppressor of interommatidial cell | increased number | somatic clone | pupal stage phenotype of ex^AP50

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a suppressor | partially of interommatidial cell | increased number | somatic clone | pupal stage phenotype of hpo^5.1

(Gaspar et al., 2015)

Zyx^Δ41 is a suppressor | partially of wing disc | third instar larval stage phenotype of wts^P2/Df(3R)Exel8194

(Gaspar et al., 2015)

NOT Suppressor of

Statement

Reference

Zyx^Δ41/Zyx^Δ41 is a non-suppressor of eye disc | third instar larval stage phenotype of ft⁸/ft^G-rv

(Gaspar et al., 2015)

Zyx^Δ41/Zyx^Δ41 is a non-suppressor of wing disc | third instar larval stage phenotype of ft⁸/ft^G-rv

(Gaspar et al., 2015)

Zyx^Δ41 is a non-suppressor of interommatidial cell | increased number | somatic clone | pupal stage phenotype of ft⁸

(Gaspar et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The supernumerary inter-ommatidial cells in the pupal retina phenotype characteristic for ft^G-rv/ft^G-rv eyes (created by the EGUF method in otherwise heterozygous animals) can be partially suppressed by combination with Zyx^Δ41/Zyx^Δ41 but this does not affect the size of the mutant adult eyes.

The low rate of survival of ft^G-rv/ft⁸ transheterozygotes to third instar larval stage (and never to adulthood), the loss of photoreceptor cells in the eye discs or the overgrowth of the wing disc cannot be suppressed by combination with Zyx^Δ41/Zyx^Δ41.

The supernumerary inter-ommatidial cells in the pupal retina phenotype characteristic for ft⁸/ft⁸ eyes (created by the EGUF method in otherwise heterozygous animals) cannot be restored by combination with Zyx^Δ41/Zyx^Δ41.

The supernumerary inter-ommatidial cells in the pupal retina as well as the smaller size and altered eye shape in adults characteristic for ex^e1/ex^e1 eyes (created by the EGUF method in otherwise heterozygous animals) can be suppressed by combination with Zyx^Δ41/Zyx^Δ41.

The low survival rate of ex^e1/ex^e1 mutants to third instar larval stage can be improved by combination with Zyx^Δ41/Zyx^Δ41 and some of the double mutants can survive even to adulthood. The decreased number of photoreceptor cells in the eye discs characteristic for ex^e1/ex^e1 mutants is also restored in the surviving ex^e1;Zyx^Δ41 double mutant third instar larvae.

The overgrowth of the wing discs characteristic for ex^e1/ex^AP50 transheterozygotes can be suppressed by combination with Zyx^Δ41/Zyx^Δ41.

The supernumerary inter-ommatidial cells in the pupal retina phenotype characteristic for ex^AP50/ex^AP50 eyes (created by the EGUF method in otherwise heterozygous animals) can be suppressed by combination with Zyx^Δ41/Zyx^Δ41.

The supernumerary inter-ommatidial cells in the pupal retina phenotype characteristic for hpo^5.1/hpo^5.1 eyes (created by the EGUF method in otherwise heterozygous animals) can be partially suppressed by combination with Zyx^Δ41/Zyx^Δ41 but the overgrowth and folded shape of the adult eyes cannot be rescued.

The reduced size of both eye and wing discs characteristic for d¹/d^GC13 transheterozygote third instar larvae is further enhanced by combination with Zyx^Δ41/Zyx^Δ41.

The small eye phenotype characteristic for d^GC13/d^GC13 adult eyes (created by the EGUF method in otherwise heterozygous animals) cannot be suppressed by combination with Zyx^Δ41/Zyx^Δ41.

(Gaspar et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Zyx^Δ41 is rescued by Zyx^{Δ208-231.UAS}/Scer\GAL4^nub.PU

(Gaspar et al., 2015)

Zyx^Δ41 is rescued by Scer\GAL4^nub.PU/Zyx^UAS.cGa

(Gaspar et al., 2015)

Zyx^Δ41 is rescued by Scer\GAL4^nub.PU/Zyx^{UAS.Tag:Myr(Src42A)}

(Gaspar et al., 2015)

Zyx^Δ41 is rescued by Scer\GAL4^nub.PU/Zyx^{Δ208-231.UAS.Tag:Myr(Src42A)}

(Gaspar et al., 2015)

Partially rescued by

Zyx^Δ41 is partially rescued by Zyx^Ubi-p63E.PG

(Gaspar et al., 2015)

Comments

Expression of Zyx^Ubi-p63E.PG in the Zyx^Δ41/Zyx^Δ41 mutant background can partially restore the decreased body weight and fully suppress the small and narrow wing phenotype characteristic for Zyx^Δ41/Zyx^Δ41 adults.

The small wing phenotype displayed by Zyx^Δ41/Zyx^Δ41 adults can be fully rescued by expression of Zyx^Scer\UAS.cGa under the control of Scer\GAL4^nub.PU in the mutant background, resulting in adult wings comparable in size to wild-type.

Flies expressing Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^nub.PU in a Zyx^Δ41/Zyx^Δ41 background have adult wings that are significantly bigger in size than wild-type.

Flies expressing Zyx^{Δ208-231.Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^nub.PU in a Zyx^Δ41/Zyx^Δ41 background have adult wings that are comparable in size to wild-type.

Flies expressing Zyx^{Δ208-231.Scer\UAS} under the control of Scer\GAL4^nub.PU in a Zyx^Δ41/Zyx^Δ41 background have adult wings that are comparable in size to wild-type.

The small wing phenotype displayed by Zyx^Δ41/Zyx^Δ41 adults can be fully rescued by expression of Zyx^{Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^nub.PU in the mutant background, resulting in adult wings that are significantly bigger in size than wild-type.

The small wing phenotype displayed by Zyx^Δ41/Zyx^Δ41 adults can be fully rescued by expression of Zyx^{Δ208-231.Scer\UAS.T:Myr-Src42A} under the control of Scer\GAL4^nub.PU in the mutant background, resulting in adult wings comparable in size to wild-type.

The small wing phenotype displayed by Zyx^Δ41/Zyx^Δ41 adults can be fully rescued by expression of Zyx^{Δ208-231.Scer\UAS} under the control of Scer\GAL4^nub.PU in the mutant background, resulting in adult wings that are comparable in size to wild-type.

(Gaspar et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Zyx^Δ41

zyx^Δ41

(Gaspar et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

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