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| Term | TALEN | ID (Ontology) | FBcv:0003009 (FlyBase CV) | |||||||||
| Definition | Sequence change caused by the cleavage of specific DNA sequences by transcription activator-like effector nucleases (TALENs). TALEN proteins comprise a DNA binding domain fused to the catalytic domain of the FokI endonuclease. The DNA binding domain contains a repetitive region within the middle of the protein, consisting of tandem repeats of 34 amino acid segments, with each of these segments recognizing a single base pair. The specificity of the binding is determined by two hypervariable amino acids in positions 12 and 13 (repeat-variable diresidues). Cleavage requires dimerization of FokI. The endonuclease is directed to a target region by a pair of TALENs which bind to adjacent sequences separated by a spacer region, allowing dimerization and promoting cleavage.[ PubMed:10954606 PubMed:20660643 ] | |||||||||||
| Also Known As | "change induced by TALE endonuclease cleavage" | |||||||||||
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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origin of mutation |__site specific cleavage |__TALEN 107 rec. |
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| Is a | site specific cleavage | ||
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