FlyBase Allele Report: Dmel\ds[GD2646]

General Information

Symbol

Dmel\ds^GD2646

Species

D. melanogaster

Name

FlyBase ID

FBal0209438

Feature type

allele

Associated gene

Dmel\ds

Associated Insertion(s)

Carried in Construct

P{GD2646}

Also Known As

UAS-ds-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2646}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2646

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | adult stage, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

abnormal size | adult stage, with Scer\GAL4⁶³⁸

(Revilla-Yates et al., 2015)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

(Dietzl et al., 2007)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4⁶³⁸

(Revilla-Yates et al., 2015)

wing, with Scer\GAL4^Bx-MS1096

(Dietzl et al., 2007)

wing, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

wing blade posterior compartment, with Scer\GAL4^hh.PU

(Revilla-Yates et al., 2015)

wing hair, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

wing vein, with Scer\GAL4^en.PU

(Revilla-Yates et al., 2015)

Detailed Description

Statement

Reference

The expression of ds^GD2646 under the combined control of Scer\GAL4^nub.PU and Dicer-2 transgene (for an efficient RNAi) leads to a significant increase in total wing area and disturbs the overall wing hair polarity, as compared to controls, as compared to controls.

(Misra and Irvine, 2016)

Expressing ds^GD2646 under the control of Scer\GAL4^en.PU results in mild wing defects, including larger anterior-posterior cross-vein distance, as compared to controls.

Expressing ds^GD2646 under the control of Scer\GAL4^hh.PU leads to wings with smaller relative posterior area in association with smaller third instar larval wing discs, as compared to wild-type controls.

Expressing ds^GD2646 under the control of Scer\GAL4^en.PU has no apparent cell death effect in the posterior compartment of third instar larval wing discs, as compared to controls.

(Revilla-Yates et al., 2015)

Adults expressing ds^GD2646 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in an enlarged notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2646

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^nub.PU, ds^GD2646 has abnormal size | adult stage phenotype, suppressible by Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

NOT Suppressor of

Statement

Reference

ds^GD2646, Scer\GAL4^nub.PU is a non-suppressor of abnormal size | adult stage phenotype of Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^nub.PU, ds^GD2646 has wing phenotype, suppressible by Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

NOT Suppressor of

Statement

Reference

ds^GD2646, Scer\GAL4^nub.PU is a non-suppressor of wing phenotype of Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of ds^GD2646 and Dlish^HMS01708 under the combined control of Scer\GAL4^nub.PU and Dicer-2 transgene (for an efficient RNAi) results in a small wing area phenotype similar to that observed upon expression of Dlish^HMS01708 alone, instead of the big wing area phenotype observed upon the expression of ds^GD2646 alone.

(Misra and Irvine, 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v4313

w¹¹¹⁸; P{GD2646}v4313/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2646

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ds^GD2646

(González-Morales et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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