FlyBase Allele Report: Dmel\ft[GD881]

General Information

Symbol

Dmel\ft^GD881

Species

D. melanogaster

Name

FlyBase ID

FBal0209603

Feature type

allele

Associated gene

Dmel\ft

Associated Insertion(s)

Carried in Construct

P{GD881}

Also Known As

UAS-RNAi-fat, UAS-fat-RNAi, ft RNAi, UAS-ft-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD881}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD881

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal planar polarity | adult stage, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

abnormal size, with Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

crossvein, with Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Huang et al., 2013)

wing, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

wing, with Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

wing hair, with Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Detailed Description

Statement

Reference

The expression of ft^GD881 under the combined control of Scer\GAL4^nub.PU and Dicer-2 transgene (for an efficient RNAi) leads to a significant increase in total wing area and disturbs the overall wing hair polarized orientation, as compared to controls.

(Misra and Irvine, 2016)

Expression of ft^GD881 under the control of Scer\GAL4^Bx-MS1096 results in wing overgrowth.

(Huang et al., 2013)

Expression of ft^GD881 under the control of Scer\GAL4^salm-459.2 does not affect wing hair polarity.

(Hogan et al., 2011)

Expression of ft^GD881 (together with Dcr-2^Scer\UAS.cDa) using Scer\GAL4^nub-AC-62 results in overgrown wings. Crossvein spacing is also affected.

(Rauskolb et al., 2011)

Expression of ft^GD881 (co-expressed with Dcr-2^Scer\UAS.cDa) under the control of Scer\GAL4^repo.PU has no detectable effect on glial cell number in the third instar larval eye disc.

(Reddy and Irvine, 2011)

Adults expressing ft^GD881 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 40-50% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^nub.PU, ft^GD881 has abnormal size | adult stage phenotype, suppressible by Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Scer\GAL4^nub.PU, ft^GD881 has abnormal planar polarity | adult stage phenotype, suppressible by Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Scer\GAL4^nub-AC-62, ft^GD881 has abnormal size phenotype, suppressible by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

Scer\GAL4^nub-AC-62, ft^GD881 has abnormal size phenotype, suppressible by Scer\GAL4^nub-AC-62/jub^GD7185

(Rauskolb et al., 2011)

NOT Suppressor of

Statement

Reference

ft^GD881, Scer\GAL4^nub.PU is a non-suppressor of abnormal size | adult stage phenotype of Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Other

Statement

Reference

Scer\GAL4^salm-459.2, ft^GD881, pk³⁰ has visible phenotype

(Hogan et al., 2011)

Scer\GAL4^salm-459.2, ft^GD881, pk³⁰ has abnormal planar polarity phenotype

(Hogan et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^nub.PU, ft^GD881 has wing phenotype, suppressible by Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Scer\GAL4^nub.PU, ft^GD881 has wing hair phenotype, suppressible by Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Scer\GAL4^Bx-MS1096, ft^GD881 has wing phenotype, suppressible by par-1^HMS00405/Scer\GAL4^Bx-MS1096

(Huang et al., 2013)

Scer\GAL4^nub-AC-62, ft^GD881 has wing phenotype, suppressible by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

Scer\GAL4^nub-AC-62, ft^GD881 has wing phenotype, suppressible by Scer\GAL4^nub-AC-62/jub^GD7185

(Rauskolb et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^nub-AC-62, ft^GD881 has crossvein phenotype, non-suppressible by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

NOT Suppressor of

Statement

Reference

ft^GD881, Scer\GAL4^nub.PU is a non-suppressor of wing phenotype of Dlish^HMS01708, Scer\GAL4^nub.PU

(Misra and Irvine, 2016)

Other

Statement

Reference

Scer\GAL4^salm-459.2, ft^GD881, pk³⁰ has wing hair phenotype

(Hogan et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of ft^GD881 and Dlish^HMS01708 under the combined control of Scer\GAL4^nub.PU and Dicer-2 transgene (for an efficient RNAi) results in wing phenotypes that are similar to those observed upon expression of Dlish^HMS01708 alone (i.e. small wings and virtually no wing hair orientation defects), instead of similar to those observed upon expression of ft^GD881 alone (i.e. big wings and severe wing hair orientation defects).

(Misra and Irvine, 2016)

Expression of par-1^HMS00405 suppresses the overgrowth phenotype seen in wings expressing ft^GD881 under the control of Scer\GAL4^Bx-MS1096.

(Huang et al., 2013)

Expression of ft^GD881 under the control of Scer\GAL4^salm-459.2 in a pk³⁰ mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4^salm-459.2 expression domain.

(Hogan et al., 2011)

The wing overgrowth phenotype associated with Scer\GAL4^nub-AC-62-mediated expression of ft^GD881 (together with Dcr-2^Scer\UAS.cDa) is suppressed by co-expression of Zyx102EF^NIG.32018R, resulting in wings of similar size to those of animals that only express Zyx102EF^NIG.32018R. However, the influence of ft^GD881 expression on crossvein spacing is not suppressed by Zyx102EF^NIG.32018R expression.

The wing overgrowth phenotype associated with Scer\GAL4^nub-AC-62-mediated expression of ft^GD881 (together with Dcr-2^Scer\UAS.cDa) is suppressed by co-expression of jub^GD7185.

(Rauskolb et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v9396

w¹¹¹⁸; P{GD881}v9396

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ft^GD881

(González-Morales et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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