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Citation
Peng, Y.H., Yang, W.K., Lin, W.H., Lai, T.T., Chien, C.T. (2009). Nak regulates Dlg basal localization in Drosophila salivary gland cells.  Biochem. Biophys. Res. Commun. 382(1): 108--113.
FlyBase ID
FBrf0207737
Publication Type
Research paper
Abstract

Protein trafficking is highly regulated in polarized cells. During development, how the trafficking of cell junctional proteins is regulated for cell specialization is largely unknown. In the maturation of Drosophila larval salivary glands (SGs), the Dlg protein is essential for septate junction formation. We show that Dlg was enriched in the apical membrane domain of proximal cells and localized basolaterally in distal mature cells. The transition of Dlg distribution was disrupted in nak mutants. Nak associated with the AP-2 subunit alpha-Ada and the AP-1 subunit AP-1gamma. In SG cells disrupting AP-1 and AP-2 activities, Dlg was enriched in the apical membrane. Therefore, Nak regulates the transition of Dlg distribution likely through endocytosis of Dlg from the apical membrane domain and transcytosis of Dlg to the basolateral membrane domain during the maturation of SGs development.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Biochem. Biophys. Res. Commun.
    Title
    Biochemical and Biophysical Research Communications
    Publication Year
    1959-
    ISBN/ISSN
    0006-291X
    Data From Reference
    Alleles (10)
    Genes (11)
    Physical Interactions (4)
    Cell Lines (1)
    Transgenic Constructs (4)