FlyBase Allele Report: Dmel\AP-2α[06694]

(Gross et al., 2013, Song and Lu, 2012, Spradling et al., 1999, Forjanic et al., 1997, Gonzalez-Gaitan and Jackle, 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates retinal disease

modeled by norpA^EE5

(Orem et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval salivary gland | third instar larval stage (with AP-2α³)

(Peng et al., 2009)

larval brain

(Song and Lu, 2012)

larval neuroblast | increased number

(Song and Lu, 2012)

retina | conditional | somatic clone

(Orem et al., 2006)

synapse

(Gonzalez-Gaitan and Jackle, 1997)

Detailed Description

Statement

Reference

AP-2α⁰⁶⁶⁹⁴ mutants do not display defects in mushroom body lobe structure.

(Gross et al., 2013)

α-Adaptin⁰⁶⁶⁹⁴ mutant larval brains contain supernumerary neuroblasts but few differentiated neurons in the central brain.

(Song and Lu, 2012)

Salivary gland cell growth (which normally occurs in the third larval instar, starting at the distal end of the gland) is defective in α-Adaptin⁰⁶⁶⁹⁴/α-Adaptin³ third instar larvae.

(Peng et al., 2009)

α-Adaptin⁰⁶⁶⁹⁴ somatic mutant eye clones from animals raised in the dark and then exposed to 5 days of constant room light show signs of retinal degeneration, and large holes are evident in eye sections.

(Orem et al., 2006)

Slow moving larvae die as pupae. FM1-43 assay demonstrates the mutation impairs the formation of endocytotic synaptic vesicles.

(Gonzalez-Gaitan and Jackle, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

AP-2α⁰⁶⁶⁹⁴ has abnormal neuroanatomy | recessive phenotype, suppressible by Scer\GAL4^insc-Mz1407/Delta^RNAi.UAS.cUa

(Song and Lu, 2012)

AP-2α⁰⁶⁶⁹⁴ has hyperplasia | recessive phenotype, suppressible by Scer\GAL4^insc-Mz1407/Delta^RNAi.UAS.cUa

(Song and Lu, 2012)

Enhancer of

Statement

Reference

AP-2α⁰⁶⁶⁹⁴ is an enhancer of abnormal neuroanatomy phenotype of AP-2α::numb^PTB.UAS.GFP, Scer\GAL4^insc-Mz1407

(Song and Lu, 2012)

NOT Suppressor of

Statement

Reference

AP-2α⁰⁶⁶⁹⁴ is a non-suppressor of abnormal neuroanatomy | recessive | somatic clone phenotype of spdo^G104

(Song and Lu, 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

AP-2α⁰⁶⁶⁹⁴ has larval neuroblast | increased number phenotype, suppressible by Scer\GAL4^insc-Mz1407/Delta^RNAi.UAS.cUa

(Song and Lu, 2012)

AP-2α⁰⁶⁶⁹⁴ has larval brain phenotype, suppressible by Scer\GAL4^insc-Mz1407/Delta^RNAi.UAS.cUa

(Song and Lu, 2012)

Enhancer of

Statement

Reference

AP-2α⁰⁶⁶⁹⁴ is an enhancer of mushroom body vertical lobe phenotype of X11L⁸⁰

(Gross et al., 2013)

AP-2α⁰⁶⁶⁹⁴ is an enhancer of mushroom body medial lobe phenotype of X11L⁸⁰

(Gross et al., 2013)

AP-2α⁰⁶⁶⁹⁴ is an enhancer of intermediate neuronal progenitor phenotype of AP-2α::numb^PTB.UAS.GFP, Scer\GAL4^insc-Mz1407

(Song and Lu, 2012)

Suppressor of

Statement

Reference

AP-2α⁰⁶⁶⁹⁴ is a suppressor of retina | conditional | somatic clone phenotype of norpA^EE5

(Orem et al., 2006)

AP-2α⁰⁶⁶⁹⁴ is a suppressor of eye photoreceptor cell | conditional | somatic clone phenotype of norpA^EE5

(Orem et al., 2006)

AP-2α⁰⁶⁶⁹⁴ is a suppressor of rhabdomere | conditional | somatic clone phenotype of norpA^EE5

(Orem et al., 2006)

NOT Suppressor of

Statement

Reference

AP-2α⁰⁶⁶⁹⁴ is a non-suppressor of larval neuroblast | somatic clone phenotype of spdo^G104

(Song and Lu, 2012)

AP-2α⁰⁶⁶⁹⁴ is a non-suppressor of intermediate neuronal progenitor | somatic clone phenotype of spdo^G104

(Song and Lu, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

A AP-2α⁰⁶⁶⁹⁴ homozygous mutant background enhances the dendritic mislocalisation of axonal membrane proteins and structural defects in the mushroom body of X11L⁸⁰ mutants.

(Gross et al., 2013)

Expression of α-Adaptin::numb^{PTB.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^insc-Mz1407 in a α-Adaptin⁰⁶⁶⁹⁴ mutant background leads to further depletion of all type II neuroblasts.

The neuroblast-depletion phenotype is still observed in the type II neuroblast lineages when spdo^G104 mutant clones are induced in a α-Adaptin⁰⁶⁶⁹⁴ mutant background.

Expression of Dl^TRiP.cUa in neuroblast lineages via Scer\GAL4^insc-Mz1407 completely suppresses the neuroblast overproliferation phenotype of α-Adaptin⁰⁶⁶⁹⁴ mutants.

(Song and Lu, 2012)

In α-Adaptin⁰⁶⁶⁹⁴,norpA⁷ double mutant eye clones, from animals raised in the dark and then exposed to 5 days of constant room light, retinal degeneration is rescued and eyes show wild-type morphology.

(Orem et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

AP-2α^ear26

(Berdnik et al., 2002)

AP-2α^ear4

(Berdnik et al., 2002)

AP-2α^ear5

(Berdnik et al., 2002)

Rescued by

AP-2α⁰⁶⁶⁹⁴ is rescued by Scer\GAL4^insc-Mz1407/AP-2α^{ΔEar5.UAS.GFP}

(Song and Lu, 2012)

AP-2α⁰⁶⁶⁹⁴ is rescued by Scer\GAL4^insc-Mz1407/AP-2α^{ΔEar.UAS.GFP}

(Song and Lu, 2012)

AP-2α⁰⁶⁶⁹⁴ is rescued by Scer\GAL4^insc-Mz1407/AP-2α^{Trunk.UAS.GFP}

(Song and Lu, 2012)

Not rescued by

AP-2α⁰⁶⁶⁹⁴ is not rescued by Scer\GAL4^insc-Mz1407/AP-2α^N2.UAS.GFP

(Song and Lu, 2012)

AP-2α⁰⁶⁶⁹⁴ is not rescued by Scer\GAL4^insc-Mz1407/AP-2α^{ΔNTΔEar.UAS.GFP}

(Song and Lu, 2012)

Comments

Neuroblast-specific expression of α-Adaptin^{ΔNTΔEar.Scer\UAS.T:Avic\GFP} via Scer\GAL4^insc-Mz1407 fails to rescue the neuroblast overproliferation phenotype of α-Adaptin⁰⁶⁶⁹⁴ mutants.

Neuroblast-specific expression of α-Adaptin^{N2.Scer\UAS.T:Avic\GFP} via Scer\GAL4^insc-Mz1407 fails to rescue the neuroblast overproliferation phenotype ofα-Adaptin⁰⁶⁶⁹⁴ mutants.

Neuroblast-specific expression of α-Adaptin^{Trunk.Scer\UAS.T:Avic\GFP} via Scer\GAL4^insc-Mz1407 completely rescues the neuroblast overproliferation phenotype of α-Adaptin⁰⁶⁶⁹⁴ mutants.

Neuroblast-specific expression of α-Adaptin^{ΔEar.Scer\UAS.T:Avic\GFP} via Scer\GAL4^insc-Mz1407 completely rescues the neuroblast overproliferation phenotype of α-Adaptin⁰⁶⁶⁹⁴ mutants.

Neuroblast-specific expression of α-Adaptin^{ΔEar5.Scer\UAS.T:Avic\GFP} via Scer\GAL4^insc-Mz1407 completely rescues the neuroblast overproliferation phenotype of α-Adaptin⁰⁶⁶⁹⁴ mutants.

(Song and Lu, 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12319

cn¹ P{PZ}AP-2α⁰⁶⁶⁹⁴/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: ex⁰¹²⁷⁰. Complements: ex⁰²²⁷⁸. Complements: lwr⁰²⁸⁵⁸. Complements: dock⁰⁴⁷²³. Complements: ds⁰⁵¹⁴². Complements: Gsc⁰⁵³⁴¹. Complements: lwr⁰⁵⁴⁸⁶. Complements: S⁰⁷⁰⁵⁶. Complements: kis⁰⁷⁸¹². Complements: Hop^k00616. Complements: kis^k11324. Complements: ebi^k16213. Complements: RpI135^k16513.

(BDGP Project Members, 1994-1999)

Excision of the P-element results in normal adults indicating the P{PZ} insertion causes the lethal phenotype.

(Gonzalez-Gaitan and Jackle, 1997)

Excision of the P{PZ} element results in viability demonstrating the insertion to be responsible for lethality.

(Forjanic et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

AP-2α⁰⁶⁶⁹⁴

AP2⁰⁶⁶⁹⁴

(Gross et al., 2013)

D-αAda¹

(Boehm and Bonifacino, 2002, Gonzalez-Gaitan and Jackle, 1997)

ada¹

(Liu et al., 2017, Berdnik et al., 2002)

l(2)06694

(Spradling et al., 1999)

l(2)06694⁰⁶⁶⁹⁴

l(2)6694

(Forjanic et al., 1997)

α-Adaptin⁰⁶⁶⁹⁴

α-Adaptin⁶⁶⁹⁴

(Kraut et al., 2001)

α-ada¹

(Chance and Bashaw, 2015, Matta et al., 2012, Song and Lu, 2012, Peng et al., 2009, Peng et al., 2009)

α-adaptin⁰⁶⁶⁹⁴

(Orem et al., 2006)

αAdaptin⁰⁶⁶⁹⁴

(Wu et al., 2017)

Name Synonyms

α-adaptin06694

(Gross et al., 2013)

Secondary FlyBase IDs

References (18)

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