FlyBase Allele Report: Dmel\trc[K122A+T453A.UAS.L]

General Information

Symbol

Dmel\trc^{K122A+T453A.UAS.L}

Species

D. melanogaster

Name

FlyBase ID

FBal0215630

Feature type

allele

Associated gene

Dmel\trc

Associated Insertion(s)

Carried in Construct

P{UAS-trc.L.K122A+T453A}

Key Links

Transgenic product class

missense_variant

(He et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(He et al., 2005)

Mutagen

in vitro construct

(He et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-trc.L.K122A+T453A}

Cytology

Description

Amino acid replacement: K122A.

(He et al., 2005)

Amino acid replacement: T435A.

(He et al., 2005)

UASt regulatory sequences drive expression of a mutated form of the long isoform of trc with the above amino acid substitution.

(He et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

trc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Pink1^B9

(Wu et al., 2013)

modeled by Pink1^RNAi.UAS.cYa

(Wu et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of trc^{K122A+T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544 does not cause a strong multiple wing hair phenotype.

(He et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, trc^{K122A+T453A.UAS.L} has visible phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Enhancer of

Statement

Reference

Scer\GAL4^ple.PF/trc^{K122A+T453A.UAS.L} is an enhancer of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Wu et al., 2013)

trc^{K122A+T453A.UAS.L}, Scer\GAL4^Mhc.PW is an enhancer of abnormal flight phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

trc^{K122A+T453A.UAS.L}, Scer\GAL4^Mhc.PW is an enhancer of visible phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, trc^{K122A+T453A.UAS.L} has wing phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, trc^{K122A+T453A.UAS.L} has mitochondrion phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Enhancer of

Statement

Reference

Scer\GAL4^ple.PF/trc^{K122A+T453A.UAS.L} is an enhancer of mitochondrion phenotype of Pink1^B9

(Wu et al., 2013)

Scer\GAL4^ple.PF/trc^{K122A+T453A.UAS.L} is an enhancer of dopaminergic PPL1 neuron phenotype of Pink1^B9

(Wu et al., 2013)

trc^{K122A+T453A.UAS.L}, Scer\GAL4^Mhc.PW is an enhancer of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

trc^{K122A+T453A.UAS.L}, Scer\GAL4^Mhc.PW is an enhancer of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of trc^{K122A+T453A.Scer\UAS} does not suppress the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^insc-Mz1407.

(Lee et al., 2013)

Co-expression of trc^{K122A+T453A.Scer\UAS} enhances the defects in flight ability caused by expression of Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW.

The abnormal wing posture phenotype seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW is enhanced by co-expression of trc^{K122A+T453A.Scer\UAS}.

The mitochondrial aggregation phenotype seen in the muscles of flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW is enhanced by co-expression of trc^{K122A+T453A.Scer\UAS}.

The mitochondrial aggregation phenotype seen in the dopaminergic neurons of Pink1^B9 flies is enhanced by expression of trc^{K122A+T453A.Scer\UAS} under the control of Scer\GAL4^ple.PF.

The loss of dopaminergic neurons in the PPL1 cluster seen in Pink1^B9 flies is enhanced by expression of trc^{K122A+T453A.Scer\UAS} under the control of Scer\GAL4^ple.PF.

(Wu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PARK2^Scer\UAS.cYa completely blocks the ability of trc^{K122A+T453A.Scer\UAS} to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW.

(Wu et al., 2013)

Complementation and Rescue Data

Fails to rescue

trc^{K122A+T453A.UAS.L}/Scer\GAL4^Act5C.PI fails to rescue trc^S066917a

(He et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

trc^{K122A+T453A.Scer\UAS.L}

trc^{K122A+T453A.Scer\UAS}

trc^{K122A+T453A.UAS.L}

trc^K122A+T453A

(He et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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