Ef2b, EF2, Elongation factor 2b, Elongation factor 2
Gene model reviewed during 5.55
Gene model reviewed during 5.46
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.56
Phosphorylation by EF-2 kinase completely inactivates eEF2.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eEF2 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\eEF2 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
FlyBase curator comment: Through to FB2013_04, there were two Gene Reports for genes encoding 'eukaryotic elongation factor 2' in FlyBase. 'Ef2b' (FBgn0000559, at 39E7) was annotated as CG2238 and widely referenced. In contrast, 'Ef2a' (FBgn0000558, at 85C) was unannotated and associated with a single paper (FBrf0056636). Re-examination of this paper finds that only a single Dmel gene is mentioned therein (referred to generically as 'EF-2'). Moreover, an earlier paper that specifically studied the Dmel EF2 gene (FBrf0050717) stated that it "is a single copy gene residing at position 39E-F" (i.e. 'Ef2b'). Therefore, the 'Ef2a' gene record has been obsoleted, and the 'Ef2b' gene has been renamed as 'EF2' following the nomenclature used in the original paper to characterize the gene (FBrf0050717).
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
Area matching Drosophila EF2 gene. Acc. No. X15805.
Phylogeny is inferred from EF-2/EF-G sequences from different species using alternative treeing methods.
Encodes elongation factor 2 (EF2), which catalyzes the GTP-dependent translocation of tRNA from the aminoacyl to the peptidyl site of the ribosome; also catalyzes hydrolysis of GTP. Expression becomes detectable by four hours of development and persists into adulthood.