FlyBase Gene Report: Dmel\mRRF2

General Information

Symbol

Dmel\mRRF2

Species

D. melanogaster

Name

mitochondrial ribosome recycling factor 2

Annotation Symbol

CG31159

Feature Type

protein_coding_gene

FlyBase ID

FBgn0051159

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

mitochondrial ribosome recycling factor 2 (mRRF2) encodes a mitochondrial elongation factor that catalyzes the translocation of the mRNAs and tRNAs along the ribosomes via GTP hydrolysis. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

EF-G2

Key Links

Genomic Location

Cytogenetic map

94B6-94B6

Sequence location

3R:22,613,686..22,616,622 [+]

Recombination map

3-75

RefSeq locus

NT_033777 REGION:22613686..22616622

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables GTP binding

inferred from electronic annotation with InterPro:IPR000795, InterPro:IPR005225

(InterPro Project Members, 2004-)

enables GTPase activity

inferred from electronic annotation with InterPro:IPR000795

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000562984

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:Q969S9

(Gene Ontology Curators, 2002-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in mitochondrial translation

inferred from biological aspect of ancestor with PANTHER:PTN000562984

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:Q969S9

(Gene Ontology Curators, 2002-)

involved_in ribosome disassembly

inferred from electronic annotation with InterPro:IPR030851

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:Q969S9

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000754007

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in mitochondrial matrix

inferred by curator from GO:0032543

(FlyBase Curators, 2016-)

located_in mitochondrion

inferred from sequence or structural similarity with SGD:S000003638

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR030851

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

MITOCHONDRIAL TRANSLATION RECYCLING FACTORS

TRANSLATIONAL GTPASES

Protein Family (UniProt)

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily. (Q9VCX4)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

GTPase activity
RHEA 19669:

Summaries

Gene Snapshot

Gene Group (FlyBase)

MITOCHONDRIAL TRANSLATION RECYCLING FACTORS -: Mitochondrial translation recycling factors function in the disassembly of the ribosome-mRNA-tRNA complex following the termination of translation in mitochondria. (Adapted from PMID:22172991).
TRANSLATIONAL GTPASES -: Translational GTPases are a family of GTPases that facilitate and control protein synthesis on the ribosome. (Adapted from PMID:26971860).

Protein Function (UniProtKB)

Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.

(UniProt, Q9VCX4)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\mRRF2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VCX4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.52

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.53

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mRRF2-RA

FBtr0084270

NM_170028

2481

740

mRRF2-RB

FBtr0344226

NM_001300539

2476

565

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

mRRF2-PA

FBpp0083663

82.0

740

5.81

FBpp0310632

63.2

565

5.09

A0A0B4LIH0

NP_001287468

AHN57467

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mRRF2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

EF-G2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.99

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mRRF2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mRRF2

View exonic expression by tissue for Dmel\mRRF2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42670/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051159

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051159

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051159

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31159

Flygut - An atlas of the Drosophila adult midgut

FBgn0051159

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mRRF2

Transgenic constructs containing regulatory region of mRRF2

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6273

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal, with Scer\GAL4^tin.CΔ4

mRRF2^GD10182

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

mRRF2^GD10182

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (12)

Hsap\GFM2

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (7)

Rnor\Gfm2

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (7)

Mmus\Gfm2

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (7)

Xtro\gfm2

11 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (11)

Drer\gfm2

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\Y119D3B.14

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (8)

Agam\RRF2M_ANOGA

12 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (16)

Atha\SCO1

5 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (8)

Scer\MEF2

12 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\SPBC660.10

11 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (5)

Ecol\fusA

2 of 11

Yes

Ecol\prfC

2 of 11

Yes

Ecol\bipA

1 of 11

Ecol\infB

1 of 11

Ecol\lepA

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:mRRF2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

waw

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

GFM2; GTP dependent ribosome recycling factor mitochondrial 2

model of combined oxidative phosphorylation deficiency 39

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GFM2; GTP dependent ribosome recycling factor mitochondrial 2

14 of 14

606544

COMBINED OXIDATIVE PHOSPHORYLATION DEFICIENCY 39; COXPD39

combined oxidative phosphorylation deficiency 39

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0051159

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Mitochondrial translation termination

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-75

Cytogenetic map

94B6-94B6

Sequence location

3R:22,613,686..22,616,622 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

94B6-94B6

Limits computationally determined from genome sequence between P{lacW}Dph5^L4910 and P{lacW}Gclm^L0580

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-75

(FlyBase, 2016)

3-77.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

31159R-2

v104187

v46144

w¹¹¹⁸ P{GD10182}v46144

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (15)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH61333

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0051159

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0051159

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Annotation CG6943 split into CG31159 and CG31161 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: EF-G2 CG31159

(Trivigno and Haerry, 2011)

Source for identity of: mRRF2 EF-G2

(Marygold et al., 2017)

Nomenclature comments

Etymology

Named after a synonym of its human ortholog.

(Trivigno and Haerry, 2011)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG31159

(Marygold et al., 2017, Aradska et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Trivigno and Haerry, 2011, Keleman et al., 2009.8.5)

CG6943

EF-G2

(Kwon et al., 2013)

dEF-G2

(Trivigno and Haerry, 2011)

mRRF2

(Marygold et al., 2017)

Name Synonyms

Elongation Factor G2

mitochondrial ribosome recycling factor 2

Secondary FlyBase IDs

FBgn0038995

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42670

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

BI631042

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VCX4

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VCX4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4LIH0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VCX4

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42670/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051159

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051159

FlyMine - An integrated database for Drosophila genomics

FBgn0051159

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31159

MARRVEL_MODEL - MARRVEL (model organism gene)

42670

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0051159

DroID - A comprehensive database of gene and protein interactions.

FBgn0051159

DRSC - Results frm RNAi screens

FBgn0051159

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

EF-G2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051159

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0051159

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31159

Flygut - An atlas of the Drosophila adult midgut

FBgn0051159

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0051159

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009837

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Mitochondrial translation termination

References (23)

The following external sites may use different assemblies or annotations than FlyBase.

1.99

Report Sections