Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from 0-21hr old Drosophila embryo cDNA library).
Two-hybrid system:Two-hybrid system: yeast DNA BD/GAL4-AD
T191R,C209L, coordinates relative to Mob2-PD
T191R,H197Q, coordinates relative to Mob2-PD
R223E,L226P, coordinates relative to Mob2-PD
H289Q coordinates relative to Mob2-PD
L181P,L187P,A188I, coordinates relative to Mob2-PD
Two-hybrid system: yeast DNA BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct
