FlyBase Gene Report: Dmel\Nurf-38

General Information

Symbol

Dmel\Nurf-38

Species

D. melanogaster

Name

Nucleosome remodeling factor - 38kD

Annotation Symbol

CG4634

Feature Type

protein_coding_gene

FlyBase ID

FBgn0016687

Gene Model Status

Current

Stock Availability

10 publicly available

Enzyme Name (EC)

inorganic diphosphatase (3.6.1.1)

Gene Summary

Component of NURF (nucleosome remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin (PubMed:9784495). NURF is required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis (PubMed:8521501, PubMed:9784495). Inorganic pyrophosphatase (PPase), hydrolyzes inorganic pyrophosphate to inorganic phosphate, essential for driving critical biosynthetic reactions including transcription, replication, and DNA repair (PubMed:9784495). (UniProt, O77460)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

NURF, NURF38, p38, dNURF, l(2)k16102

Key Links

Genomic Location

Cytogenetic map

60D5-60D5

Sequence location

2R:24,558,967..24,561,087 [-]

Recombination map

2-107

RefSeq locus

NT_033778 REGION:24558967..24561087

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables inorganic diphosphate phosphatase activity

inferred from direct assay

(Gdula et al., 1998)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables inorganic diphosphate phosphatase activity

inferred from biological aspect of ancestor with PANTHER:PTN000032431

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR008162, InterPro:IPR036649

(InterPro Project Members, 2004-)

enables magnesium ion binding

inferred from electronic annotation with InterPro:IPR008162, InterPro:IPR036649

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in chromatin remodeling

inferred from direct assay

(Mizuguchi et al., 2001, Gdula et al., 1998)

involved_in nucleosome organization

inferred from direct assay

(Hamiche et al., 1999)

involved_in positive regulation of DNA-templated transcription

inferred from direct assay

(Mizuguchi et al., 2001)

involved_in positive regulation of transcription by RNA polymerase II

inferred from genetic interaction with FLYBASE:EcR; FB:FBgn0000546

(Badenhorst et al., 2005)

involved_in regulation of hemocyte proliferation

inferred from mutant phenotype

(Sinenko et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in phosphate-containing compound metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000032431

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR008162, InterPro:IPR036649

(InterPro Project Members, 2004-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Gdula et al., 1998)

located_in nucleus

inferred from direct assay

(Gdula et al., 1998)

part_of NURF complex

inferred from direct assay

(Kwon et al., 2009, Gdula et al., 1998)

inferred from physical interaction with FLYBASE:Iswi; FB:FBgn0011604

(Gdula et al., 1998)

inferred from physical interaction with FLYBASE:Caf1-55; FB:FBgn0263979

(Gdula et al., 1998)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from electronic annotation with InterPro:IPR008162, InterPro:IPR036649

(InterPro Project Members, 2004-)

is_active_in mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000032435

(GO Reference Genome Project, 2011-)

located_in mitochondrion

inferred from sequence or structural similarity with UniProtKB:Q9H2U2

(Gene Ontology Curators, 2002-)

part_of NURF complex

traceable author statement

(Badenhorst et al., 2002)

Gene Group (FlyBase)

NUCLEOSOME REMODELING FACTOR

UNCLASSIFIED ACID ANHYDRIDE HYDROLASES

Protein Family (UniProt)

Belongs to the PPase family. (O77460)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

inorganic diphosphate phosphatase activity

diphosphate + H2O = 2 phosphate + H(+) (3.6.1.1)

RHEA 24576:

Summaries

Gene Group (FlyBase)

NUCLEOSOME REMODELING FACTOR -: Nucleosome Remodeling Factor (NURF) is an ISWI ATPase-containing chromatin remodeling complex that catalyzes ATP-dependent nucleosome sliding. (Adapted from FBrf0228922 and FBrf0228920).
UNCLASSIFIED ACID ANHYDRIDE HYDROLASES -: This group comprises acid anhydride hydrolases that do not classify under other groups in FlyBase.

Protein Function (UniProtKB)

(UniProt, O77460)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nurf-38 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry O77460)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nurf-38-RA

FBtr0072343

NM_079125

1178

338

Nurf-38-RB

FBtr0273253

NM_001144286

1144

290

Nurf-38-RC

FBtr0305985

NM_001259565

1281

290

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Nurf-38-PA

FBpp0072250

37.9

338

7.01

FBpp0271761

32.6

290

5.41

FBpp0297127

32.5

290

5.66

A0A0B4K7G9

NP_001246494

AFH08247

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

290 (aa); 40 (kD observed); 33 (kD predicted)

(Gdula et al., 1998)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the NURF complex composed of Caf1-55, E(bx), Nurf-38 and Iswi.

(UniProt, O77460)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nurf-38 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.25

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Nurf-38 protein is observed in both the cytoplasm and the nucleus.

(Gdula et al., 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Gdula et al., 1998)

located_in nucleus

inferred from direct assay

(Gdula et al., 1998)

part_of NURF complex

inferred from direct assay

(Kwon et al., 2009, Gdula et al., 1998)

inferred from physical interaction with FLYBASE:Iswi; FB:FBgn0011604

(Gdula et al., 1998)

inferred from physical interaction with FLYBASE:Caf1-55; FB:FBgn0263979

(Gdula et al., 1998)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Nurf-38 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nurf-38

View exonic expression by tissue for Dmel\Nurf-38

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016687

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37922/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016687

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016687

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016687

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4634

Flygut - An atlas of the Drosophila adult midgut

FBgn0016687

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nurf-38

Transgenic constructs containing regulatory region of Nurf-38

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)Px2

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Gene not disrupted in

Df(2R)bw^VDe2LPx^KR

(Spradling et al., 1999, Beaton, 1997.12.27)

In(2LR)Px⁴

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Nurf-38^GD1625

lethal, with Scer\GAL4^Act5C.PI

lethal, with Scer\GAL4^pnr-MD237

Nurf-38^GD1625

lethal | recessive

Nurf-38^k16102

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Nurf-38^GD1625

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Nurf-38^GD1625

Sterility

Allele

female fertile, with Scer\GAL4^nanos.PG

Nurf-38^KK102637

female semi-sterile, with Scer\GAL4^nanos.PG

Nurf-38^GD1625

Other Phenotypes

Allele

abnormal starvation stress response | adult stage, with Nurf-38^JF01299, Scer\GAL4^ppl.PU

Nurf-38^KK102637

abnormal starvation stress response | adult stage, with Nurf-38^KK102637, Scer\GAL4^ppl.PU

Nurf-38^JF01299

decreased size | larval stage, with Nurf-38^JF01299, Scer\GAL4^ppl.PU

Nurf-38^KK102637

decreased size | larval stage, with Nurf-38^KK102637, Scer\GAL4^ppl.PU

Nurf-38^JF01299

increased mortality | adult stage, with Nurf-38^JF01299, Scer\GAL4^ppl.PU

Nurf-38^KK102637

increased mortality | adult stage, with Nurf-38^KK102637, Scer\GAL4^ppl.PU

Nurf-38^JF01299

some die during pupal stage, with Scer\GAL4^ey.PH

visible | adult stage, with Scer\GAL4^en.PU

Nurf-38^GL00369

visible | adult stage, with Scer\GAL4^ey.PH

visible | adult stage, with Scer\GAL4^nub.PK

Nurf-38^JF01299

visible | larval stage, with Nurf-38^JF01299, Scer\GAL4^ppl.PU

Nurf-38^KK102637

visible | larval stage, with Nurf-38^KK102637, Scer\GAL4^ppl.PU

Nurf-38^JF01299

Phenotype manifest in

Allele

embryonic/larval dorsal tracheal branch | larval stage, with Scer\GAL4^btl.PS

embryonic/larval fat body | larval stage, with Nurf-38^JF01299, Scer\GAL4^ppl.PU

Nurf-38^KK102637

embryonic/larval fat body | larval stage, with Nurf-38^KK102637, Scer\GAL4^ppl.PU

Nurf-38^JF01299

eye, with Scer\GAL4^ey.PH

larva, with Nurf-38^JF01299, Scer\GAL4^ppl.PU

Nurf-38^KK102637

larva, with Nurf-38^KK102637, Scer\GAL4^ppl.PU

Nurf-38^JF01299

lipid droplet | decreased number, with Nurf-38^JF01299, Scer\GAL4^ppl.PU

Nurf-38^KK102637

lipid droplet | decreased number, with Nurf-38^KK102637, Scer\GAL4^ppl.PU

Nurf-38^JF01299

mitochondrion | larval stage, with Nurf-38^JF01299, Scer\GAL4^ppl.PU

Nurf-38^KK102637

mitochondrion | larval stage, with Nurf-38^KK102637, Scer\GAL4^ppl.PU

Nurf-38^JF01299

posterior wing margin, with Scer\GAL4^en.PU

Nurf-38^GL00369

terminal tracheal cell | larval stage, with Scer\GAL4^btl.PS

Nurf-38^GL00369

wing blade, with Scer\GAL4^en.PU

Nurf-38^GL00369

wing blade, with Scer\GAL4^nub.PK

Nurf-38^JF01299

wing vein, with Scer\GAL4^en.PU

Nurf-38^GL00369

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\PPA1

14 of 14

Yes

Hsap\PPA2

13 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (3)

Rnor\Ppa2

13 of 14

Yes

Rnor\Ppa1

12 of 14

Yes

Rnor\Sar1a

11 of 14

Yes

Mus musculus (laboratory mouse) (2)

Mmus\Ppa1

14 of 14

Yes

Mmus\Ppa2

12 of 14

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\ppa2

11 of 13

Yes

Xtro\ppa1

7 of 13

Yes

Xtro\capn6

1 of 13

Danio rerio (Zebrafish) (3)

Drer\ppa1b

14 of 14

Yes

Drer\ppa1a

12 of 14

Yes

Drer\ppa2

12 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\pyp-1

13 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP003398

11 of 12

Yes

Arabidopsis thaliana (thale-cress) (6)

Atha\PPa6

13 of 13

Yes

Atha\PPa1

3 of 13

Yes

Atha\PPa2

3 of 13

Yes

Atha\PPa3

3 of 13

Yes

Atha\PPa4

3 of 13

Yes

Atha\PPa5

3 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\IPP1

13 of 13

Yes

Scer\PPA2

7 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\SPAC23C11.05

11 of 12

Yes

Spom\SPAC3A12.02

7 of 12

Yes

Escherichia coli (enterobacterium) (1)

Ecol\ppa

3 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Nurf-38. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 4 )

Allele

Disease

Interaction

References

Nurf-38^JF01299

ameliorates Alzheimer's disease

modeled by Hsap\MAPT^R406W.UAS

(Frost et al., 2014)

Nurf-38^k16102

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Nurf-38^NIG.4634R

ameliorates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Nurf-38^RNAi.UAS.cUa

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Pragati and Sarkar, 2025)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PPA1; inorganic pyrophosphatase 1

14 of 14

179030

PPA2; inorganic pyrophosphatase 2

13 of 14

609988

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Nurf-38 - Caf1-55

ion exchange chromatography, cosedimentation, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot

(Gdula et al., 1998, Tsukiyama and Wu, 1995, Tsukiyama et al., 1995)

Nurf-38 - E(bx)

ion exchange chromatography, cosedimentation, molecular weight estimation by staining, anti tag coimmunoprecipitation, western blot

(Kwon et al., 2009, Tsukiyama and Wu, 1995, Tsukiyama et al., 1995)

Nurf-38 - Fkbp14

experimental knowledge based

(Guruharsha et al., 2011)

Nurf-38 - Iswi

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot, ion exchange chromatography, cosedimentation, molecular weight estimation by staining

(Doyen et al., 2015, Gdula et al., 1998, Tsukiyama and Wu, 1995, Tsukiyama et al., 1995)

Nurf-38 - MBD-R2

coimmunoprecipitation, western blot

(Lam et al., 2019)

Nurf-38 - Nap1

anti bait coimmunoprecipitation, western blot

(Stephens et al., 2006)

Nurf-38 - Rcd5

anti bait coimmunoprecipitation, western blot

(Lam et al., 2019)

Nurf-38 - Su(var)2-HP2

anti bait coimmunoprecipitation, western blot

(Stephens et al., 2006)

Nurf-38 - dgt1

anti tag coimmunoprecipitation, western blot

(Lam et al., 2019)

Nurf-38 - nsl1

anti bait coimmunoprecipitation, western blot

(Lam et al., 2019)

Nurf-38 - pzg

anti bait coimmunoprecipitation, western blot

(Kugler and Nagel, 2010)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

EcR

enhanceable

Nurf-38

(Badenhorst et al., 2005)

trc

enhanceable

Nurf-38

(Hudson et al., 2023)

External Data

Subunit Structure (UniProtKB)

Component of the NURF complex composed of Caf1-55, E(bx), Nurf-38 and Iswi.

(UniProt, O77460 )

Linkouts

BioGRID - A database of protein and genetic interactions.

63494

DroID - A comprehensive database of gene and protein interactions.

FBgn0016687

MIST (protein-protein) - An integrated Molecular Interaction Database

37922

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Oxidative phosphorylation

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Pyrophosphate hydrolysis

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-107

Cytogenetic map

60D5-60D5

Sequence location

2R:24,558,967..24,561,087 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60D5-60D5

Limits computationally determined from genome sequence between P{PZ}bs⁰³²⁶⁷&P{lacW}l(2)k10502^k10502 and P{lacW}Nurf-38^k16102

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60C-60C

(Gdula, 1998.8.20, Gdula, 1998.8.20, Gdula et al., 1998)

60D5-60D6

(determined by in situ hybridisation)

(Spradling et al., 1999)

60D5-60D6

(Beaton, 1997.12.27, BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-107

(FlyBase, 2016)

2-111.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

31341

y¹ v¹; P{TRiP.JF01299}attP2

12206

y¹ w^67c23; P{lacW}Nurf-38^k16102/SM6a

35444

y¹ sc^* v¹ sev²¹; P{TRiP.GL00369}attP2

Kyoto

111452

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Nurf-38^k16102 /CyO y⁺

NIG-Fly

4634R-2

P{NIG.4634R}2

4634R-3

P{NIG.4634R}3

GL00369

y¹ sc^* v¹ sev²¹; P{TRiP.GL00369}attP2

2RG-0946

y² VhaAC39-1^cho-1 v¹; P{U6-Nurf-38.NIG.sgRNA}attP40/CyO

VDRC

v103776

P{KK102637}VIE-260B

v3200

w¹¹¹⁸; P{GD1625}v3200/TM3

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (305)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE37074

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016687

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016687

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Gdula et al., 1998)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Area matching Drosophila EST AI062013. This EST matches Drosophila gene NURF-38. Acc. No. AF085601.

(Davies, 2001.3.15)

The NURF complex (composed of Caf1, E(bx), Iswi and Nurf-38 proteins) can remodel chromatin and stimulate transcription in vitro irrespective of the acetylation status of histones.

(Mizuguchi et al., 2001)

Nurf-38 may serve a structural or regulatory role in the nucleosome remodeling factor (NURF) complex.

(Gdula et al., 1998)

NURF, a protein complex of four subunits Caf1, Nurf-38, E(bx) and Iswi, is able to facilitate transcription mediated by a Scer\GAL4 derivative carrying a constitutively activating region from Hsf. Once nucleosome remodelling by the DNA binding factor is accomplished a high level of NURF activity is not continuously required for recruitment of the general transcription machinery and transcription initiation. NURF is able to assist gene activation in a chromatin context: remodel nucleosomes for gene activation in chromatin.

(Mizuguchi et al., 1997)

Nucleosome remodelling factor is composed of at least four polypeptides that act in concert with the Trl transcription factor at the Hsp70 promoter. Nucleosome remodelling factor acts directly on the nucleosome to perturb its structure.

(Tsukiyama and Wu, 1995)

Relationship to Other Genes

Source for database merge of

Source for merge of: Nurf-38 l(2)k16102

(Spradling et al., 1999)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Nurf-38 CG4634

(Wang, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

(Hochheimer and Tjian, 2003)

CG4634

(National Institute of Genetics Fly Stocks, 2022-, Çiçek et al., 2016, Hosono et al., 2015, Cammarato et al., 2011, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Reumer et al., 2009, Ni et al., 2008, Nakahara et al., 2005)

N38

(Schuettengruber et al., 2007)

NURF 38

(Dietz et al., 2015, Tsukiyama et al., 1995)

NURF-38

(Reumer et al., 2009, Hochheimer et al., 2002, Corona et al., 2000, Hamiche et al., 1999, Gdula et al., 1998)

NURF38

(Liu et al., 2020, Lam et al., 2019, Doyen et al., 2015, Frost et al., 2014, Alkhatib and Landry, 2011, Schuettengruber et al., 2011, Clapier and Cairns, 2009, Kwon et al., 2009, Bouazoune and Brehm, 2006, Corona and Tamkun, 2004, Lewis et al., 2004, Langst and Becker, 2001, Xiao et al., 2001)

Nurf 38

(Kugler and Nagel, 2010)

Nurf38

(Hudson et al., 2023, Vidaurre and Chen, 2021, Yin et al., 2021, Yao et al., 2018, Ghasemi et al., 2015, Kugler and Nagel, 2010, Sinenko et al., 2010, Badenhorst et al., 2005)

PPA1

(Yin et al., 2021)

dNURF

(Emerson, 2002, Fry and Peterson, 2001, Boyer et al., 2000, Peterson, 2000)

l(2)k16102

(Beaton, 1997.12.27, BDGP Project Members, 1994-1999)

p38

(Ryu et al., 2010, Stephens et al., 2006, Schwanbeck et al., 2004, Hochheimer et al., 2002)

p38/NURF-38

(Hochheimer et al., 2002)

Name Synonyms

Inorganic pyrophosphatase

(Creagh et al., 2009)

Inorganic pyrophosphatase 1

(Yin et al., 2021)

Nucleosome remodeling factor

(Kingston et al., 1996)

Nucleosome remodeling factor - 38kD

(Cammarato et al., 2011, Tsukiyama and Wu, 1995)

Nucleosome remodeling factor-38 kDa

(Reumer et al., 2009)

pyrophosphatase

(Eberharter and Becker, 2004)

Secondary FlyBase IDs

FBgn0021828

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37922

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

O77460

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O77460

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

O77460

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016687

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37922/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016687

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016687

FlyMine - An integrated database for Drosophila genomics

FBgn0016687

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4634

MARRVEL_MODEL - MARRVEL (model organism gene)

37922

Linkouts

BioGRID - A database of protein and genetic interactions.

63494

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016687

DroID - A comprehensive database of gene and protein interactions.

FBgn0016687

DRSC - Results frm RNAi screens

FBgn0016687

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016687

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016687

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4634

Flygut - An atlas of the Drosophila adult midgut

FBgn0016687

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0016687

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004566

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Oxidative phosphorylation

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

37922

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Pyrophosphate hydrolysis

References (156)

The following external sites may use different assemblies or annotations than FlyBase.

0.25

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