FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Iswi, Nurf-38
FlyBase ID
FBig0000095001
Interaction Network
Interactions Browser links
Iswi network
Nurf-38 network
Reported Interactions
FBrf0086062-5.CH.MW
Reference
Tsukiyama et al., 1995
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Iswi 

140 Kd

neutral component
Iswi 

140 Kd

neutral component
Iswi 

140 Kd

neutral component
Nurf-38 

38 Kd

neutral component
Nurf-38 

38 Kd

neutral component
Nurf-38 

38 Kd

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Nuclear extract was purified through several different chromatographic steps, selecting for nucleosome disruption activity.

(Tsukiyama et al., 1995)

Identity of 140 kDa band as Iswi confirmed by both western blot and mass spectrometry.

(Tsukiyama et al., 1995)

E(bx), Iswi, Caf1 and Nurf-38 cosediment as part of the approximately 500 kDa NURF complex.

(Tsukiyama et al., 1995)

Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.

(Tsukiyama et al., 1995)
FBrf0086063-5.CH.MW
Reference
Tsukiyama and Wu, 1995
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Iswi 

140 Kd

neutral component
Iswi 

140 Kd

neutral component
Iswi 

140 Kd

neutral component
Nurf-38 

38 Kd

neutral component
Nurf-38 

38 Kd

neutral component
Nurf-38 

38 Kd

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Nuclear extract was purified through several different chromatographic steps, selecting for nucleosome disruption activity.

(Tsukiyama and Wu, 1995)

E(bx), Iswi, Caf1 and Nurf-38 cosediment as part of the approximately 500 kDa NURF complex.

(Tsukiyama and Wu, 1995)

Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.

(Tsukiyama and Wu, 1995)
FBrf0105229-1.coIP.WB
Reference
Gdula et al., 1998
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nurf-38 

p38

bait
Iswi 

ISWI

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interact as part of the NURF chromatin remodeling complex.

(Gdula et al., 1998)

Bait endogenous to crude nuclear fraction.

(Gdula et al., 1998)

Prey endogenous to crude nuclear fraction.

(Gdula et al., 1998)
FBrf0105229-4.coIP.WB
Reference
Gdula et al., 1998
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Iswi 

ISWI

bait
Nurf-38 

p38

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interact as part of the NURF chromatin remodeling complex.

(Gdula et al., 1998)

Bait endogenous to crude nuclear fraction.

(Gdula et al., 1998)

Prey endogenous to crude nuclear fraction.

(Gdula et al., 1998)
FBrf0230177-12.coIP.MS
Reference
Doyen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Iswi 

ISWI

bait
Nurf-38 

NURF38

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Doyen et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)