FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
trc, fry
FlyBase ID
FBig0000113210
Interaction Network
Interactions Browser links
trc network
fry network
Reported Interactions
FBrf0184141-1.coIP.WB
Reference
He et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
fry 

Fry

bait

Myc tag

fry 
trc 

Trc

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fry 
amino terminal fragment
sufficient binding region
aa 1-1637

coordinates relative to AA-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(He et al., 2005)
FBrf0210776-1.Y2H
Reference
Fang et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trc 

Trc

bait

fused to GAL4 DNA-binding domain

trc 
trc 
fry 

Fry

prey

fused to GAL4 activation domain

fry 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fry 
N-terminal domain
sufficient binding region
aa 1-1637
trc 
interaction domain
sufficient binding region
aa 1-350

coordinates relative to trc-PB

trc 
interaction domain
necessary binding region
aa 337-350

coordinates relative to trc-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Fang et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD. C-terminal deletions of trc bind fry strongly in the two-hybrid assay while full length trc does not suggesting the C-terminus of trc inhibits interaction with fry.

(Fang et al., 2010)
FBrf0210776-2.coIP.WB
Reference
Fang et al., 2010
Description
physical association
Assay
Collection
Source/Stage
wing disc / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trc 

Trc

bait

FLAG tag

fry 

Fry

prey

GFP or Myc tag

fry 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fry 
N-terminal domain
sufficient binding region
aa 1-1637
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Fang et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)