FlyBase Gene Report: Dmel\RhoL

General Information

Symbol

Dmel\RhoL

Species

D. melanogaster

Name

Rho-like

Annotation Symbol

CG9366

Feature Type

protein_coding_gene

FlyBase ID

FBgn0014380

Gene Model Status

Current

Stock Availability

16 publicly available

Gene Summary

Essential for the maturation of hemocytes. (UniProt, Q24192)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Mes1, DRhoL, Drac3

Key Links

Genomic Location

Cytogenetic map

85D18-85D18

Sequence location

3R:9,497,101..9,502,581 [-]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:9497101..9502581

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (21 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables GTP binding

inferred from biological aspect of ancestor with PANTHER:PTN000632671

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR003578, InterPro:IPR005225

(InterPro Project Members, 2004-)

enables GTPase activity

inferred from biological aspect of ancestor with PANTHER:PTN000632671

(GO Reference Genome Project, 2011-)

traceable author statement

(Newsome et al., 2000)

inferred from electronic annotation with InterPro:IPR001806

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P63000

(Sasamura et al., 1997)

enables protein kinase binding

inferred from biological aspect of ancestor with PANTHER:PTN001530052

(GO Reference Genome Project, 2011-)

Biological Process (15 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in border follicle cell migration

inferred from mutant phenotype

(Medioni and Noselli, 2005)

involved_in hemocyte development

inferred from mutant phenotype

(Sasamura et al., 1997)

involved_in hemocyte migration

inferred from mutant phenotype

(Siekhaus et al., 2010)

involved_in melanotic encapsulation of foreign target

inferred from mutant phenotype

(Howell et al., 2012)

involved_in mesoderm development

inferred from expression pattern

(Furlong et al., 2001)

involved_in positive regulation of cell-cell adhesion

inferred from mutant phenotype

(Siekhaus et al., 2010)

Terms Based on Predictions or Assertions (9 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from biological aspect of ancestor with PANTHER:PTN000632671

(GO Reference Genome Project, 2011-)

involved_in cell chemotaxis

inferred from biological aspect of ancestor with PANTHER:PTN008604698

(GO Reference Genome Project, 2011-)

involved_in cortical cytoskeleton organization

inferred from biological aspect of ancestor with PANTHER:PTN001530052

(GO Reference Genome Project, 2011-)

involved_in establishment or maintenance of cell polarity

inferred from biological aspect of ancestor with PANTHER:PTN001530052

(GO Reference Genome Project, 2011-)

involved_in motor neuron axon guidance

inferred from biological aspect of ancestor with PANTHER:PTN002784640

(GO Reference Genome Project, 2011-)

involved_in Rac protein signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN002784640

(GO Reference Genome Project, 2011-)

involved_in regulation of actin cytoskeleton organization

inferred from biological aspect of ancestor with PANTHER:PTN001530052

(GO Reference Genome Project, 2011-)

involved_in regulation of cell shape

inferred from biological aspect of ancestor with PANTHER:PTN001530052

(GO Reference Genome Project, 2011-)

involved_in small GTPase-mediated signal transduction

inferred from electronic annotation with InterPro:IPR003578

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasmic vesicle

inferred from biological aspect of ancestor with PANTHER:PTN001530052

(GO Reference Genome Project, 2011-)

is_active_in cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN001530052

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000632671

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

RHO GTPASES

Protein Family (UniProt)

Belongs to the small GTPase superfamily. Rho family. (Q24192)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

GTPase activity
RHEA 19669:

Summaries

Gene Group (FlyBase)

RHO GTPASES -: Rho (Ras Homologous) GTPases are members of the Ras superfamily of small GTPases. They are best characterized for their roles in regulating actin organization. (Adapted from PMID:15731001).

Protein Function (UniProtKB)

Essential for the maturation of hemocytes.

(UniProt, Q24192)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RhoL for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q24192)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RhoL-RA

FBtr0082124

NM_079568

1041

190

RhoL-RB

FBtr0305340

NM_001260074

1205

214

Additional Transcript Data and Comments

Reported size (kB)

1.1 (northern blot)

(Sasamura et al., 1997)

1.5 (northern blot)

(Casal and Leptin, 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

RhoL-PA

FBpp0081602

21.9

190

9.01

FBpp0293883

24.8

214

9.71

D3DMW7

NP_001247003

AFH06321

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

190 (aa)

(Sasamura et al., 1997)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RhoL using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.59

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

ventral furrow

(Casal and Leptin, 1996)

embryonic stage 4 -- 6

head mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 5

mesoderm anlage

(Stathopoulos et al., 2002, Casal and Leptin, 1996)

embryonic stage 5 -- 11

embryonic/larval head mesoderm derived hemocyte

(Siekhaus et al., 2010)

embryonic stage 5 -- 17

hemocyte

(Casal and Leptin, 1996)

embryonic stage 6 -- 17

mesoderm

(Casal and Leptin, 1996)

embryonic stage 7 -- 8

head mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

head mesoderm

(Fisher et al., 2012)

embryonic stage 11

head mesoderm

(Sasamura et al., 1997)

embryonic stage 11 -- 12

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

embryonic stage 12

mesoderm

(Siekhaus et al., 2010)

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Sasamura et al., 1997)

embryonic stage 6 -- 10

ventral furrow | anterior

(Sasamura et al., 1997)

embryonic stage 11

hemocyte

(Sasamura et al., 1997)

embryonic stage 12 -- 17

organism | ubiquitous

(Sasamura et al., 1997)

Additional Descriptive Data

RhoL mRNA is expressed specifically in head mesoderm where haemocytes originate, starting at stage 5 and continuing in all haemocytes through stage 11. After this, RhoL mRNA ceases expression in haemocytes and expression begins in more posterior mesoderm.

(Siekhaus et al., 2010)

RhoL transcript is detected in all developmental stages. Using in situ hybridization to embryos, RhoL was detected from stage 6 to 10, in the anterior part of the ventral furrow. This expression extended caudally and dorsolaterally, and RhoL localized to the cephalic mesoderm after stage 10. After stage 11, expression levels decreased, but became ubiquitous. RhoL colocalizes with a hemocyte marker (peroxidasin), and is thus being expressed in hemocyte precursor cells.

(Sasamura et al., 1997)

Transcripts are expressed in ventral cells of the blastoderm embryo. The anterior border coincides with the anterior limit of the ventral furrow. Expression is strong in the part of the ventral furrow anterior to the cephalic furrow and weak in the trunk region. In later embryos, expression is observed in migrating hemocytes.

(Casal and Leptin, 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\RhoL in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RhoL

View exonic expression by tissue for Dmel\RhoL

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0014380

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41136/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0014380

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0014380

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0014380

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9366

Flygut - An atlas of the Drosophila adult midgut

FBgn0014380

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\RhoL

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RhoL

Transgenic constructs containing regulatory region of RhoL

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3L)997

(Murphy and Montell, 1996)

Df(3R)by416

(Sasamura et al., 1997)

Gene not disrupted in

Df(3R)by62

(Sasamura et al., 1997)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^198Y

RhoL^V20.UAS

lethal, with Scer\GAL4³⁰⁶

RhoL^V20.UAS

lethal, with Scer\GAL4⁴⁵⁸

RhoL^V20.UAS

lethal, with Scer\GAL4^hs.PB

RhoL^V20.UAS

viable

RhoL^10-161

viable, with Scer\GAL4^198Y

RhoL^N25.UAS

viable, with Scer\GAL4²¹²

viable, with Scer\GAL4³⁰⁶

RhoL^N25.UAS

viable, with Scer\GAL4⁴⁵⁸

RhoL^N25.UAS

viable, with Scer\GAL4^hs.PB

RhoL^N25.UAS

Other Phenotypes

Allele

abnormal immune response | larval stage

RhoL^EP3312

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

RhoL^EP888

Phenotype manifest in

Allele

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

RhoL^EP888

border follicle cell, with Scer\GAL4^slbo.2.6

RhoL^N25.UAS

follicle cell

RhoL^V20.hs

hemocyte | embryonic stage

hemocyte | embryonic stage (with Df(3R)by416)

RhoL^XA12

larval intersegmental nerve, with Scer\GAL4^elav-C155

RhoL^EP888

nurse cell

polar follicle cell, with Scer\GAL4^slbo.2.6

RhoL^N25.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (54)

Hsap\RAC1

3 of 14

Yes

Hsap\RHOA

3 of 14

Yes

Hsap\RHOB

3 of 14

Yes

Hsap\RHOD

3 of 14

Yes

Hsap\RHOH

3 of 14

Yes

Hsap\RAC2

2 of 14

Hsap\RAC3

2 of 14

Hsap\RHOBTB3

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\RND2

2 of 14

Yes

Hsap\RND3

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (20)

Rnor\Rac1

3 of 14

Yes

Rnor\Rhoa

3 of 14

Yes

Rnor\Rhob

3 of 14

Yes

Rnor\Rhoc

3 of 14

Yes

Rnor\RSA-14-44

3 of 14

Yes

Rnor\Cdc42

2 of 14

Rnor\Rac2

2 of 14

Rnor\Rhobtb3

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (22)

Mmus\4930544G11Rik

3 of 14

Yes

Mmus\Rac1

3 of 14

Yes

Mmus\Rhoa

3 of 14

Yes

Mmus\Rhob

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (37)

Xtro\rac1

2 of 13

Yes

Xtro\rhoa.2

2 of 13

Yes

Xtro\rhob

2 of 13

Yes

Xtro\rhoc

2 of 13

Yes

Xtro\rhoh

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\rhobtb3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\rras

1 of 13

Danio rerio (Zebrafish) (40)

Drer\rac1a

3 of 14

Yes

Drer\rac1b

3 of 14

Yes

Drer\rhoaa

3 of 14

Yes

Drer\rhoca

3 of 14

Yes

Drer\rhocb

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (22)

Cele\rac-2

3 of 14

Yes

Cele\rho-1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (21)

Agam\AgaP_AGAP003092

11 of 12

Yes

Agam\AgaP_AGAP001573

3 of 12

Agam\AgaP_AGAP006279

3 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (16)

Atha\ARAC1

3 of 13

Yes

Atha\ARAC5

3 of 13

Yes

Atha\ARAC9

3 of 13

Yes

Atha\RAC2

3 of 13

Yes

Atha\RAC3

3 of 13

Yes

Atha\RAC6

3 of 13

Yes

Atha\RAC10

3 of 13

Yes

Atha\ROP1

3 of 13

Yes

Atha\ROP2

3 of 13

Yes

Atha\ROP9

3 of 13

Yes

Atha\ROP10

3 of 13

Yes

Atha\AT3G51290

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (11)

Scer\CDC42

2 of 13

Yes

Scer\RHO1

2 of 13

Yes

Scer\RHO2

2 of 13

Yes

Scer\RHO3

2 of 13

Yes

Scer\RHO4

2 of 13

Yes

Scer\RHO5

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\cdc42

2 of 12

Yes

Spom\rho1

2 of 12

Yes

Spom\rho3

2 of 12

Yes

Spom\rho4

2 of 12

Yes

Spom\rho5

2 of 12

Yes

Spom\rho2

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:RhoL. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (19)

Cdc42

4 of 13

Mtl

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Ric

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

RAC1; Rac family small GTPase 1

model of autosomal dominant intellectual developmental disorder 48

IEA

(FlyBase, 2019-)

RHOH; ras homolog family member H

model of immunodeficiency 129

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAC1; Rac family small GTPase 1

3 of 14

INTELLECTUAL DEVELOPMENTAL DISORDER, AUTOSOMAL DOMINANT 48; MRD48

autosomal dominant intellectual developmental disorder 48

RHOA; ras homolog family member A

3 of 14

165390

ECTODERMAL DYSPLASIA WITH FACIAL DYSMORPHISM AND ACRAL, OCULAR, AND BRAIN ANOMALIES; EDFAOB

RHOB; ras homolog family member B

3 of 14

165370

RHOD; ras homolog family member D

3 of 14

605781

RHOH; ras homolog family member H

3 of 14

602037

IMMUNODEFICIENCY 129; IMD129

immunodeficiency 129

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RhoL - Arl8

experimental knowledge based

(Guruharsha et al., 2011)

RhoL - RhoGAP100F

pull down, anti tag western blot

(Spinner et al., 2018)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

RhoL

suppressible

shg

(Siekhaus et al., 2010)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Rap1

suppressible

RhoL

(Siekhaus et al., 2010)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0014380

MIST (protein-protein) - An integrated Molecular Interaction Database

41136

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

85D18-85D18

Sequence location

3R:9,497,101..9,502,581 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

85D18-85D18

Limits computationally determined from genome sequence between P{PZ}ps¹⁰⁶¹⁵&P{PZ}Ras85D⁰⁶⁶⁷⁷ and P{EP}CG9393^EP3122

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

67C-67C

(Montell, 1996.2.29)

85D-85D

(determined by in situ hybridisation)

(Montell, 1998.9.9, Sasamura et al., 1997)

67A-67A

(determined by in situ hybridisation)

(Murphy, 1995.5.2)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-46.1

(Comeron et al., 2012)

(Montell, 1996.2.29)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

4848

w¹¹¹⁸; P{hs-RhoL.N25}AM1

4851

w¹¹¹⁸; P{UAS-RhoL.V20}AM1

4850

w¹¹¹⁸; P{hs-RhoL.V20}AM1/CyO

4849

w¹¹¹⁸; P{UAS-RhoL.N25}AM1/CyO

32841

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00532}attP2

33723

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00605}attP2

Kyoto

107908

w¹¹¹⁸; P{UAS-RhoL.V20}AM1

107907

w¹¹¹⁸; P{UAS-RhoL.N25}AM1/CyO

9366R-3

v330166

v102461

v318269

PBac{fTRG00915.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (209)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD05212

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0014380

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0014380

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cells become round and detached. Kc167 cells are unaffected.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

Haemocytes in RhoL deficiency mutants do not express peroxidasin, a haemocyte-specific marker, suggesting that RhoL is essential for haemocyte differentiation.

(Sasamura et al., 1997)

Isolated from a subtractive cDNA library enriched in sequences expressed in the mesoderm.

(Casal and Leptin, 1996)

A RhoL cDNA has been cloned and sequenced. RhoL is expressed in the early mesoderm.

(Casal and Leptin, 1996)

Expression of a dominant negative RhoL transgene in ovaries causes nurse cells to collapse and fuse together concomitantly with subcortical actin breakdown. Expression of constitutively active RhoL leads to nurse cell subcortical actin breakdown and disruption of nurse cell-follicle cell contacts, followed by germ cell apoptosis. Cdc42, RhoL and Rac1 protein activities are required for normal transfer of cytoplasm from nurse cells to the oocyte. Results suggest that Rho protein activities have cell type-specific effects on morphogenesis.

(Murphy and Montell, 1996)

RhoL is a new family member of the Rho family and plays a role in oogenesis.

(Murphy and Montell, 1995)

A new member of the rho family of small GTPases.

(Murphy, 1995.5.2)

Relationship to Other Genes

Source for database merge of

Source for merge of: RhoL Rac3

(Montell, 1998.9.9)

Source for merge of: RhoL anon- EST:CL1c2

(Bayraktaroglu, 2000.11.2)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

9366

(Ravi et al., 2009)

CG9366

(Meadows, 2017.1.11, Christis and Munro, 2012, Howell et al., 2012, Li et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Gonzalez-Aguero et al., 2005, Colicelli, 2004, Lee et al., 2003, Rogers et al., 2003, Kraut et al., 2001, Kraut et al., 2001)

D-RhoL

(Ryabinina et al., 2006)

DRhoL

(Chen et al., 2002, Dobens and Raftery, 2000)

DmRHO-L

(Mentzel and Raabe, 2005)

Drac3

(Sasamura, 1999.11.29, Sasamura et al., 1997)

(Casal and Leptin, 1996)

Mes1

(Saunders et al., 2013, Stathopoulos and Levine, 2004, Stathopoulos et al., 2002)

Rac3

Rho-like

(Nicholson et al., 2008)

RhoG

(Vaqué et al., 2013)

RhoL/Rac3

(Ridley, 2001)

Rhol

anon-EST:CL1c2

rhoL

(Bischoff et al., 2021)

rhol

(Foley and O'Farrell, 2004)

rol

(Murphy and Montell, 1995)

Name Synonyms

Rho-Like

(Howell et al., 2012)

Rho-like

(Blanke and Jackle, 2006, Murphy and Montell, 1996)

rho-like

(Murphy, 1995.5.2, Murphy and Montell, 1995)

Secondary FlyBase IDs

FBgn0019864
FBgn0020619

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41136

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q24192

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q24192

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

D3DMW7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q24192

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0014380

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41136/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0014380

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0014380

FlyMine - An integrated database for Drosophila genomics

FBgn0014380

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9366

MARRVEL_MODEL - MARRVEL (model organism gene)

41136

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0014380

DroID - A comprehensive database of gene and protein interactions.

FBgn0014380

DRSC - Results frm RNAi screens

FBgn0014380

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0014380

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0014380

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9366

Flygut - An atlas of the Drosophila adult midgut

FBgn0014380

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0014380

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

41136

References (116)

The following external sites may use different assemblies or annotations than FlyBase.

-0.59

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