Mes1, Drac3, DRhoL
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RhoL using the Feature Mapper tool.
RhoL mRNA is expressed specifically in head mesoderm where haemocytes originate, starting at stage 5 and continuing in all haemocytes through stage 11. After this, RhoL mRNA ceases expression in haemocytes and expression begins in more posterior mesoderm.
RhoL transcript is detected in all developmental stages. Using in situ hybridization to embryos, RhoL was detected from stage 6 to 10, in the anterior part of the ventral furrow. This expression extended caudally and dorsolaterally, and RhoL localized to the cephalic mesoderm after stage 10. After stage 11, expression levels decreased, but became ubiquitous. RhoL colocalizes with a hemocyte marker (peroxidasin), and is thus being expressed in hemocyte precursor cells.
Transcripts are expressed in ventral cells of the blastoderm embryo. The anterior border coincides with the anterior limit of the ventral furrow. Expression is strong in the part of the ventral furrow anterior to the cephalic furrow and weak in the trunk region. In later embryos, expression is observed in migrating hemocytes.
GBrowse - Visual display of RNA-Seq signalsView Dmel\RhoL in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cells become round and detached. Kc167 cells are unaffected.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
Isolated from a subtractive cDNA library enriched in sequences expressed in the mesoderm.
Expression of a dominant negative RhoL transgene in ovaries causes nurse cells to collapse and fuse together concomitantly with subcortical actin breakdown. Expression of constitutively active RhoL leads to nurse cell subcortical actin breakdown and disruption of nurse cell-follicle cell contacts, followed by germ cell apoptosis. Cdc42, RhoL and Rac1 protein activities are required for normal transfer of cytoplasm from nurse cells to the oocyte. Results suggest that Rho protein activities have cell type-specific effects on morphogenesis.
RhoL is a new family member of the Rho family and plays a role in oogenesis.
A new member of the rho family of small GTPases.