FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Rlip, Rala
FlyBase ID
FBig0000097064
Interaction Network
Interactions Browser links
Rlip network
Rala network
Reported Interactions
FBrf0152075-1.Y2H
Reference
Naitza et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rala 

dRal

bait

fused to LexA DNA-binding domain

Rlip 

dRlip

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Naitza et al., 2002)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Naitza et al., 2002)

Positive control.

(Naitza et al., 2002)
FBrf0155960-6.Y2H
Reference
Mirey et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rala 

Ral

bait
Rlip 

RLIP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Mirey et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Mirey et al., 2003)
FBrf0161469-1.Y2H
Reference
Beller et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rala 

Ral

bait

fused to LexA DNA-binding domain

Rlip 

dRalBP

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Beller et al., 2002)

Positive control.

(Beller et al., 2002)

Bait Rala protein was mutated to be constitutively activated (G20V) and prevent the addition of the geranyl geranyl membrane targeting modifications (C198S).

(Beller et al., 2002)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Beller et al., 2002)
FBrf0190439-1.Y2H
Reference
Blanke and Jackle, 2006
Description
physical association
Assay
Collection
Source/Stage
/ embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rala 

DRal

bait

fused to GAL4 DNA-binding domain

Rlip 

DRLIP

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Blanke and Jackle, 2006)

Positive control.

(Blanke and Jackle, 2006)

Bait Rala protein carried the constitutively activating mutation G20V, as well as a C198S mutation to prevent membrane attachment via geranylgeranylation.

(Blanke and Jackle, 2006)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Blanke and Jackle, 2006)
FBrf0260862-9.coIP.MS
Reference
Brown et al., 2024
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rlip 

Rlip

bait

mCherry tag

Rala 

Rala

prey
Rala 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rala 
constitutively active residue 20
mutation increasing interaction
aa 20
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Rlip preferentially interacts with activated Rala.

(Brown et al., 2024)

Source was embryos of transgenic fly line; bait produced from endogenously tagged gene; prey produced from endogenous gene.

(Brown et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)