FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tim, CkIIα
FlyBase ID
FBig0000099440
Interaction Network
Interactions Browser links
tim network
CkIIα network
Reported Interactions
FBrf0222607-2.coIP.WB
Reference
Szabó et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CkIIα 

CK2α

bait

FLAG tag

tim 

TIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tagged transgenic construct was expressed in tim[+] cells.

(Szabó et al., 2013)

The interaction of tim with CkIIα was observed mostly in extracts from flies collected at the end of the subjective night and in the subjective morning.

(Szabó et al., 2013)

Source was adult head of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Szabó et al., 2013)
FBrf0232849-5.coIP.WB
Reference
Top et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tim 

TIM

bait

HA tag

CkIIα 

CK2α

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Top et al., 2016)
FBrf0232849-9.coIP.WB
Reference
Top et al., 2016
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tim 

TIM

bait
CkIIα 

CK2α

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Top et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)