FlyBase Allele Report: Dmel\CkIIα[RNAi.UAS]

General Information

Symbol

Dmel\CkIIα^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0212455

Feature type

allele

Associated gene

Dmel\CkIIα

Associated Insertion(s)

Carried in Construct

P{UAS-CkIIα.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Bose et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Bose et al., 2006)

Mutagen

in vitro construct

(Bose et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-CkIIα.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of double-stranded CkIIα RNA.

(Bose et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

CkIIα

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^hth-GAL4

(Bose et al., 2006)

lethal, with Scer\GAL4^tsh-md621

(Bose et al., 2006)

lethal | heat sensitive, with Scer\GAL4^h-H10

(Bose et al., 2006)

Phenotype Manifest In

eye, with CkIIα^Tik, Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

eye | heat sensitive, with Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

interommatidial bristle, with CkIIα^Tik, Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

interommatidial bristle, with Scer\GAL4^sca-537.4

(Bose et al., 2006)

interommatidial bristle | heat sensitive, with Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

macrochaeta, with CkIIα^Tik, Scer\GAL4^sca-537.4

(Bose et al., 2006)

macrochaeta, with Scer\GAL4^sca-537.4

(Bose et al., 2006)

mesothoracic bristle | increased number, with Scer\GAL4^{E(spl)m8-HLH.Exel}

(Bose et al., 2006)

microchaeta, with Scer\GAL4^sca-537.4

(Bose et al., 2006)

ommatidium, with CkIIα^Tik, Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

photoreceptor cell R8 | increased number, with Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

scutellar bristle | increased number, with Scer\GAL4^455.2

(Bose et al., 2006)

scutellar bristle | increased number, with Scer\GAL4^{E(spl)m8-HLH.Exel}

(Bose et al., 2006)

scutellar bristle | increased number, with Scer\GAL4^sca-537.4

(Bose et al., 2006)

trichogen cell, with CkIIα^Tik, Scer\GAL4^sca-537.4

(Bose et al., 2006)

trichogen cell, with Scer\GAL4^sca-537.4

(Bose et al., 2006)

Detailed Description

Statement

Reference

Expression of one copy of CkIIα^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-537.4 does not perturb the eye and leads to subtle, yet reproducible, defects in interommatidial bristle patterning. Expression of two copies of CkIIα^{dsRNA.Scer\UAS}, driven by two copies of Scer\GAL4^sca-537.4, does not affect ommatidia but leads to more severe bristle defects, such as ectopic, split and missing bristles. Expression of this transgene in a CkIIα^Tik/+ background does not lead to more extreme eye phenotypes.

Expression of CkIIα^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.3.5.Exel results in rough eyes with defective interommatidial bristle patterning in the posterior part of the eye when flies are raised at 25^oC, but results in wild-type eyes when flies are raised at 18^oC. Temperature shifts from 18^oC to 25^oC at different stages of development show that expression of CkIIα^{dsRNA.Scer\UAS} in the period that overlaps with the onset of R8 patterning (192 to 216 hours after egg laying) is sufficient to produce the rough eye phenotype, while expression before and after this period is not.

Expression of CkIIα^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.3.5.Exel in a CkIIα^Tik/+ background results in a more severe eye phenotype than expression in a wild-type background. Scer\GAL4^ey.3.5.Exel>CkIIα^{dsRNA.Scer\UAS}, CkIIα^Tik/+ eyes are rough in all sections, slightly reduced in size, display fused ommatidia, and have an interommatidial bristle patterning that is more defective.

Eye discs that express CkIIα^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.3.5.Exel display supernumerary R8 photoreceptor cells at the posterior margin of the morphogenetic furrow.

Expression of CkIIα^{dsRNA.Scer\UAS} under the control of Scer\GAL4^E(spl).Exel or Scer\GAL4^GMR.PF does not cause ommatidial or interommatidial bristle defects.

Expression of CkIIα^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-537.4 results in the formation of, typically, one extra bristle on the scutellum compared to wild-type flies. Expression with Scer\GAL4^455.2 also leads to the formation of extra scutellar bristles. Expression with Scer\GAL4^E(spl).Exel leads to the most severe bristle defect, with extra bristles present on both the scutellum and the thorax.

Expression of one copy of CkIIα^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-537.4 in a CkIIα^Tik/+ background results in split bristles and branching of the shaft cell. While this phenotype is not seen in a wild-type or CkIIα^TikR/+ background, expression of two copies of CkIIα^{dsRNA.Scer\UAS} under the control of one copy of Scer\GAL4<up>sca-537.4 does result in 'branched' bristles in a wild-type background. Expression of two copies of CkIIα^{dsRNA.Scer\UAS}, under the control of two copies of Scer\GAL4^sca-537.4, results in ectopic, split and missing macrochaetes and occasionally in split microchaetes.

(Bose et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

CkIIα^RNAi.UAS, Scer\GAL4^sca-537.4 has macrochaeta phenotype, enhanceable by E(spl)[+]/E(spl)m8-HLH¹

(Bose et al., 2006)

NOT Enhancer of

Statement

Reference

Scer\GAL4^h-H10/CkIIα^RNAi.UAS is a non-enhancer of scutellar bristle | ectopic phenotype of N^spl-1

(Kahali et al., 2009)

Suppressor of

Statement

Reference

CkIIα^RNAi.UAS, Scer\GAL4^sca-537.4 is a suppressor of scutellar bristle phenotype of E(spl)m8-HLH^UAS.cUa, Scer\GAL4^sca-537.4

(Kahali et al., 2009)

CkIIα^RNAi.UAS, Scer\GAL4^sca-537.4 is a suppressor of interommatidial bristle phenotype of E(spl)m8-HLH^UAS.cUa, Scer\GAL4^sca-537.4

(Kahali et al., 2009)

Scer\GAL4^h-H10/CkIIα^RNAi.UAS is a suppressor of eye phenotype of N^spl-1

(Kahali et al., 2009)

Scer\GAL4^h-H10/CkIIα^RNAi.UAS is a suppressor of scutellar bristle phenotype of N^spl-1

(Kahali et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^h-H10/CkIIα^RNAi.UAS is a non-suppressor of scutellar bristle | ectopic phenotype of N^spl-1

(Kahali et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of the scutellar macrochaetes and interommatidial bristles resulting from expression of E(spl)m8-HLH^Scer\UAS.cUa with Scer\GAL4^sca-537.4 is suppressed by co-expression of CkIIα^{dsRNA.Scer\UAS}.

Expression of CkIIα^{dsRNA.Scer\UAS} mediated by Scer\GAL4^h-H10 suppresses the N^spl-1/Y rough and reduced eye.

Expression of CkIIα^{dsRNA.Scer\UAS} mediated by Scer\GAL4^h-H10 suppresses the N^spl-1/Y split/missing scutellar bristle phenotype but does not modulate the N^spl-1/Y ectopic scutellar bristle phenotype.

(Kahali et al., 2009)

Expression of one copy of CkIIα^{dsRNA.Scer\UAS} under the control of one copy of Scer\GAL4^sca-537.4 in an E(spl)¹/+ background results in a split bristle phenotype, while this level of CkIIα^{dsRNA.Scer\UAS} dosage is not sufficient to induce split bristles in a wild-type background.

(Bose et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CkIIα^RNAi.UAS is rescued by Scer\GAL4^ey.3.5.Exel/CkIIα^UAS.cLa

(Bose et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

R. Jackson

(Bose et al., 2006)

Comments

Expression from the P{UAS-CkIIα.RNAi} construct produces dsRNA which results in dsRNA interference (RNAi) of the CkIIα gene.

(Bose et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CkIIα^RNAi.UAS

CkIIα^{dsRNA.Scer\UAS}

CkIIα^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

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