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General Information
Symbol
Dmel\CkIIαdsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0212455
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of double-stranded CkIIα RNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of one copy of CkIIαdsRNA.Scer\UAS under the control of Scer\GAL4sca-537.4 does not perturb the eye and leads to subtle, yet reproducible, defects in interommatidial bristle patterning. Expression of two copies of CkIIαdsRNA.Scer\UAS, driven by two copies of Scer\GAL4sca-537.4, does not affect ommatidia but leads to more severe bristle defects, such as ectopic, split and missing bristles. Expression of this transgene in a CkIIαTik/+ background does not lead to more extreme eye phenotypes.

Expression of CkIIαdsRNA.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel results in rough eyes with defective interommatidial bristle patterning in the posterior part of the eye when flies are raised at 25oC, but results in wild-type eyes when flies are raised at 18oC. Temperature shifts from 18oC to 25oC at different stages of development show that expression of CkIIαdsRNA.Scer\UAS in the period that overlaps with the onset of R8 patterning (192 to 216 hours after egg laying) is sufficient to produce the rough eye phenotype, while expression before and after this period is not.

Expression of CkIIαdsRNA.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel in a CkIIαTik/+ background results in a more severe eye phenotype than expression in a wild-type background. Scer\GAL4ey.3.5.Exel>CkIIαdsRNA.Scer\UAS, CkIIαTik/+ eyes are rough in all sections, slightly reduced in size, display fused ommatidia, and have an interommatidial bristle patterning that is more defective.

Eye discs that express CkIIαdsRNA.Scer\UAS under the control of Scer\GAL4ey.3.5.Exel display supernumerary R8 photoreceptor cells at the posterior margin of the morphogenetic furrow.

Expression of CkIIαdsRNA.Scer\UAS under the control of Scer\GAL4E(spl).Exel or Scer\GAL4GMR.PF does not cause ommatidial or interommatidial bristle defects.

Expression of CkIIαdsRNA.Scer\UAS under the control of Scer\GAL4sca-537.4 results in the formation of, typically, one extra bristle on the scutellum compared to wild-type flies. Expression with Scer\GAL4455.2 also leads to the formation of extra scutellar bristles. Expression with Scer\GAL4E(spl).Exel leads to the most severe bristle defect, with extra bristles present on both the scutellum and the thorax.

Expression of one copy of CkIIαdsRNA.Scer\UAS under the control of Scer\GAL4sca-537.4 in a CkIIαTik/+ background results in split bristles and branching of the shaft cell. While this phenotype is not seen in a wild-type or CkIIαTikR/+ background, expression of two copies of CkIIαdsRNA.Scer\UAS under the control of one copy of Scer\GAL4<up>sca-537.4 does result in 'branched' bristles in a wild-type background. Expression of two copies of CkIIαdsRNA.Scer\UAS, under the control of two copies of Scer\GAL4sca-537.4, results in ectopic, split and missing macrochaetes and occasionally in split microchaetes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The loss of the scutellar macrochaetes and interommatidial bristles resulting from expression of E(spl)m8-HLHScer\UAS.cUa with Scer\GAL4sca-537.4 is suppressed by co-expression of CkIIαdsRNA.Scer\UAS.

Expression of CkIIαdsRNA.Scer\UAS mediated by Scer\GAL4h-H10 suppresses the Nspl-1/Y rough and reduced eye.

Expression of CkIIαdsRNA.Scer\UAS mediated by Scer\GAL4h-H10 suppresses the Nspl-1/Y split/missing scutellar bristle phenotype but does not modulate the Nspl-1/Y ectopic scutellar bristle phenotype.

Expression of one copy of CkIIαdsRNA.Scer\UAS under the control of one copy of Scer\GAL4sca-537.4 in an E(spl)1/+ background results in a split bristle phenotype, while this level of CkIIαdsRNA.Scer\UAS dosage is not sufficient to induce split bristles in a wild-type background.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

R. Jackson

Comments
Comments

Expression from the P{UAS-CkIIα.RNAi} construct produces dsRNA which results in dsRNA interference (RNAi) of the CkIIα gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CkIIαdsRNA.Scer\UAS
CkIIαdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)