FlyBase Allele Report: Scer\GAL4[ey.3.5.Exel]

General Information

Symbol

Scer\GAL4^ey.3.5.Exel

Species

S. cerevisiae

Name

FlyBase ID

FBal0155840

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{ey3.5-GAL4.Exel}

Also Known As

ey-Gal4, ey3.5-Gal4, eyGAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Parks, 2004.5.12)

Progenitor genotype

Carried in construct

P{ey3.5-GAL4.Exel}

Cytology

Description

A 3.5kb ey enhancer fragment regulates expression of a GAL4 driver.

(Parks, 2004.5.12)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

eye disc anterior to the morphogenetic furrow

(Shwartz et al., 2013)

eye disc posterior to the morphogenetic furrow

(Shwartz et al., 2013)

wandering third instar larval stage

eye disc anterior to the morphogenetic furrow

(Bose et al., 2006)

Additional Information

Statement

Reference

Drives expression in eye disc epithelial cells on both sides of the morphogenetic furrow.

(FlyBase Curators, 2017)

Scer\GAL4^ey.3.5.Exel drives expression is all eye disc epithelial cells on both sides of the morphogenetic furrow.

(Shwartz et al., 2013)

Marker for

Reflects expression of

(Bloomington Drosophila Stock Center, 2017, Bose et al., 2006)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | heat sensitive, with shi^ts.UAS.cUa

(Yuva-Aydemir et al., 2011)

abnormal size | adult stage, with Hsap\UBTF^1.UAS

(Toro et al., 2018)

abnormal size | adult stage, with Larp4B^GS9719

(Funakoshi et al., 2018)

abnormal size | adult stage, with Larp4B^UAS.cFa

(Funakoshi et al., 2018)

decreased size | adult stage, with fzr^D172N.UAS

(Manivannan et al., 2022)

decreased size | adult stage, with fzr^N318K.UAS

(Manivannan et al., 2022)

decreased size | adult stage, with fzr^UAS.cMa

(Manivannan et al., 2022)

decreased size | adult stage, with Gp93^NIG.5520R

(Xu et al., 2025)

decreased size | adult stage, with Gp93^TKO.GS04507, Spyo\Cas9^UAS.P2

(Xu et al., 2025)

decreased size | adult stage, with Hsap\RBBP5^{E296D.UAS.Tag:HA}

(Huang et al., 2024)

decreased size | adult stage, with Hsap\RBBP5^UAS.Tag:HA

(Huang et al., 2024)

increased cell death | larval stage, with Gp93^NIG.5520R

(Xu et al., 2025)

increased cell death | larval stage, with Gp93^TKO.GS04507, Spyo\Cas9^UAS.P2

(Xu et al., 2025)

lethal - all die before end of pupal stage, with Hsap\UBTF^1.E210K.UAS

(Toro et al., 2018)

majority die during pupal stage, with Hsap\UBTF^1.E210K.UAS

(Toro et al., 2018)

partially lethal - majority die, with Hsap\UBTF^1.UAS

(Toro et al., 2018)

viable, with Hsap\RBBP5^{E296D.UAS.Tag:HA}

(Huang et al., 2024)

viable, with Hsap\RBBP5^{T232I.UAS.Tag:HA}

(Huang et al., 2024)

viable, with Hsap\RBBP5^UAS.Tag:HA

(Huang et al., 2024)

visible, with Hsap\MFN2^M393I.UAS

(Eschenbacher et al., 2012)

visible, with Hsap\MFN2^R400Q.UAS

(Eschenbacher et al., 2012)

visible, with kay^Fbz.UAS

(Franciscovich et al., 2008)

visible, with Marf^RNAi.UAS.cUa

(Eschenbacher et al., 2012)

visible, with Nhe2^UAS.cGa

(Grillo-Hill et al., 2015)

visible, with Ras85D^V12.S35.UAS

(Freeman et al., 2010)

visible | adult stage, with fzr^D172N.UAS

(Manivannan et al., 2022)

visible | adult stage, with fzr^N318K.UAS

(Manivannan et al., 2022)

visible | adult stage, with fzr^UAS.cMa

(Manivannan et al., 2022)

visible | adult stage, with Gp93^NIG.5520R

(Xu et al., 2025)

visible | adult stage, with Gp93^TKO.GS04507, Spyo\Cas9^UAS.P2

(Xu et al., 2025)

visible | adult stage, with Hsap\RBBP5^{E296D.UAS.Tag:HA}

(Huang et al., 2024)

visible | adult stage, with Hsap\RBBP5^UAS.Tag:HA

(Huang et al., 2024)

visible | adult stage, with Hsap\UBTF^1.UAS

(Toro et al., 2018)

visible | adult stage, with Larp4B^GS9719

(Funakoshi et al., 2018)

visible | adult stage, with Larp4B^UAS.cFa

(Funakoshi et al., 2018)

Phenotype Manifest In

adult heart, with Hsap\MFN2^R400Q.UAS

(Eschenbacher et al., 2012)

eye, with Amun^GD9779

(Shalaby et al., 2009)

eye, with CkIIα^RNAi.UAS, CkIIα^Tik

(Bose et al., 2006)

eye, with Gp93^NIG.5520R

(Xu et al., 2025)

eye, with Gp93^TKO.GS04507, Spyo\Cas9^UAS.P2

(Xu et al., 2025)

eye, with Hsap\MFN2^M393I.UAS

(Eschenbacher et al., 2012)

eye, with Hsap\MFN2^R400Q.UAS

(Eschenbacher et al., 2012)

eye, with Hsap\RBBP5^{E296D.UAS.Tag:HA}

(Huang et al., 2024)

eye, with Hsap\RBBP5^UAS.Tag:HA

(Huang et al., 2024)

eye, with Hsap\UBTF^1.UAS

(Toro et al., 2018)

eye, with kay^Fbz.UAS

(Franciscovich et al., 2008)

eye, with Larp4B^GS9719

(Funakoshi et al., 2018)

eye, with Larp4B^UAS.cFa

(Funakoshi et al., 2018)

eye, with Marf^RNAi.UAS.cUa

(Eschenbacher et al., 2012)

eye, with Nhe2^UAS.cGa

(Grillo-Hill et al., 2015)

eye, with Ras85D^V12.S35.UAS

(Freeman et al., 2010)

eye | heat sensitive, with CkIIα^RNAi.UAS

(Bose et al., 2006)

eye | heat sensitive, with CkIIα^UAS.Tik

(Bose et al., 2006)

eye-antennal disc | larval stage, with Gp93^NIG.5520R

(Xu et al., 2025)

eye-antennal disc | larval stage, with Gp93^TKO.GS04507, Spyo\Cas9^UAS.P2

(Xu et al., 2025)

eye disc | heat sensitive, with shi^ts.UAS.cUa

(Yuva-Aydemir et al., 2011)

glial cell | heat sensitive, with shi^ts.UAS.cUa

(Yuva-Aydemir et al., 2011)

head | pupal stage, with Hsap\UBTF^1.E210K.UAS

(Toro et al., 2018)

interommatidial bristle, with CkIIα^RNAi.UAS, CkIIα^Tik

(Bose et al., 2006)

interommatidial bristle | heat sensitive, with CkIIα^RNAi.UAS

(Bose et al., 2006)

interommatidial bristle | heat sensitive, with CkIIα^UAS.Tik

(Bose et al., 2006)

ommatidium, with CkIIα^RNAi.UAS, CkIIα^Tik

(Bose et al., 2006)

ommatidium, with Marf^RNAi.UAS.cUa

(Eschenbacher et al., 2012)

photoreceptor cell R8 | increased number, with CkIIα^RNAi.UAS

(Bose et al., 2006)

retina, with fzr^D172N.UAS

(Manivannan et al., 2022)

retina, with fzr^N318K.UAS

(Manivannan et al., 2022)

retina, with fzr^UAS.cMa

(Manivannan et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Larp4B^GS9719, Scer\GAL4^ey.3.5.Exel has abnormal size | adult stage phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^ey.3.5.Exel

(Funakoshi et al., 2018)

Larp4B^GS9719, Scer\GAL4^ey.3.5.Exel has visible | adult stage phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^ey.3.5.Exel

(Funakoshi et al., 2018)

Marf^RNAi.UAS.cUa, Scer\GAL4^ey.3.5.Exel has visible phenotype, suppressible by Hsap\MFN2^UAS.cDa, Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

NOT suppressed by

Statement

Reference

Marf^RNAi.UAS.cUa, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by Hsap\MFN2^R400Q.UAS, Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

Marf^RNAi.UAS.cUa, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by Hsap\MFN2^M393I.UAS, Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by kay^Fbz.UAS, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by CrebB^UAS.b.cYa, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Suppressor of

Statement

Reference

Myc^UAS.cZa, Scer\GAL4^ey.3.5.Exel is a suppressor of abnormal size | adult stage phenotype of Larp4B^GS9719, Scer\GAL4^ey.3.5.Exel

(Funakoshi et al., 2018)

Myc^UAS.cZa, Scer\GAL4^ey.3.5.Exel is a suppressor of visible | adult stage phenotype of Larp4B^GS9719, Scer\GAL4^ey.3.5.Exel

(Funakoshi et al., 2018)

Hsap\MFN2^UAS.cDa, Scer\GAL4^ey.3.5.Exel is a suppressor of visible phenotype of Marf^RNAi.UAS.cUa, Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

NOT Suppressor of

Statement

Reference

Hsap\MFN2^R400Q.UAS, Scer\GAL4^ey.3.5.Exel is a non-suppressor of visible phenotype of Marf^RNAi.UAS.cUa, Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

Hsap\MFN2^M393I.UAS, Scer\GAL4^ey.3.5.Exel is a non-suppressor of visible phenotype of Marf^RNAi.UAS.cUa, Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

kay^Fbz.UAS, Scer\GAL4^ey.3.5.Exel is a non-suppressor of visible phenotype of Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

CrebB^UAS.b.cYa, Scer\GAL4^ey.3.5.Exel is a non-suppressor of visible phenotype of Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, enhanceable by Cnx99A^EP3522, Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, enhanceable by Scer\GAL4^ey.3.5.Exel/cnk^EY06675

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, enhanceable by Pde8^EY10143, Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, enhanceable by Fkbp14^EY02405, Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, enhanceable by sty^EY11239/Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, enhanceable by Scer\GAL4^ey.3.5.Exel/pigeon^EY06835

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, enhanceable by Sdc^EY04602/Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, enhanceable by Sema2a^EY08184/Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Suppressed by

Statement

Reference

Larp4B^GS9719, Scer\GAL4^ey.3.5.Exel has eye phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^ey.3.5.Exel

(Funakoshi et al., 2018)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, suppressible by Scer\GAL4^ey.3.5.Exel/pigeon^EY06835

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, suppressible by lbm^EY02692/Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, suppressible by Pvf1^EP1624, Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, suppressible by Mbs^EP3727, Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, suppressible by Scer\GAL4^ey.3.5.Exel/spag^EY11196

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS, kay^UAS.cEa has eye phenotype, suppressible by Jra^UAS.cEa, Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

Jra^UAS.cEa, Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS has eye phenotype, suppressible by kay^UAS.cEa, Scer\GAL4^ey.3.5.Exel

(Franciscovich et al., 2008)

NOT suppressed by

Statement

Reference

Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel has eye phenotype, non-suppressible by kay^Fbz.UAS, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel has eye phenotype, non-suppressible by CrebB^UAS.b.cYa, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Enhancer of

Statement

Reference

Cnx99A^EP3522, Scer\GAL4^ey.3.5.Exel is an enhancer of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel/cnk^EY06675 is an enhancer of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Pde8^EY10143, Scer\GAL4^ey.3.5.Exel is an enhancer of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Fkbp14^EY02405, Scer\GAL4^ey.3.5.Exel is an enhancer of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

sty^EY11239/Scer\GAL4^ey.3.5.Exel is an enhancer of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel/pigeon^EY06835 is an enhancer of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Sdc^EY04602/Scer\GAL4^ey.3.5.Exel is an enhancer of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Sema2a^EY08184/Scer\GAL4^ey.3.5.Exel is an enhancer of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Suppressor of

Statement

Reference

Myc^UAS.cZa, Scer\GAL4^ey.3.5.Exel is a suppressor of eye phenotype of Larp4B^GS9719, Scer\GAL4^ey.3.5.Exel

(Funakoshi et al., 2018)

Scer\GAL4^ey.3.5.Exel/pigeon^EY06835 is a suppressor of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

lbm^EY02692/Scer\GAL4^ey.3.5.Exel is a suppressor of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Pvf1^EP1624, Scer\GAL4^ey.3.5.Exel is a suppressor of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Mbs^EP3727, Scer\GAL4^ey.3.5.Exel is a suppressor of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Scer\GAL4^ey.3.5.Exel/spag^EY11196 is a suppressor of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Jra^UAS.cEa, Scer\GAL4^ey.3.5.Exel is a suppressor of eye phenotype of Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS, kay^UAS.cEa

(Franciscovich et al., 2008)

kay^UAS.cEa, Scer\GAL4^ey.3.5.Exel is a suppressor of eye phenotype of Jra^UAS.cEa, Scer\GAL4^ey.3.5.Exel, kay^Fbz.UAS

(Franciscovich et al., 2008)

Hsap\SOD1^UAS.cPa/Scer\GAL4^ey.3.5.Exel is a suppressor of eye | somatic clone - Minute background phenotype of Opa1^f02779

(Yarosh et al., 2008)

NOT Suppressor of

Statement

Reference

kay^Fbz.UAS, Scer\GAL4^ey.3.5.Exel is a non-suppressor of eye phenotype of Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

CrebB^UAS.b.cYa, Scer\GAL4^ey.3.5.Exel is a non-suppressor of eye phenotype of Ras85D^V12.S35.UAS, Scer\GAL4^ey.3.5.Exel

(Freeman et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ey.3.5.Exel/CkIIα^UAS.cLa rescues CkIIα^RNAi.UAS

(Bose et al., 2006)

Partially rescues

spin^I.UAS/Scer\GAL4^ey.3.5.Exel partially rescues spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

8220

y¹ w¹¹¹⁸; P{ey3.5-GAL4.Exel}2

8221

y¹ w¹¹¹⁸; P{ey3.5-GAL4.Exel}1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^ey.3.5.Exel

ey^GAL4

(Huang et al., 2024)

eyeless^GAL4

(Huang et al., 2024)

Name Synonyms

Secondary FlyBase IDs

References (24)

Report Sections

Open Close